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  • Articles  (4)
  • Blackwell Publishing Ltd  (4)
  • 1995-1999  (1)
  • 1990-1994  (3)
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  • Articles  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 62 (1997), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The rigor mortis development of large Atlantic salmon was evaluated at a commercial plant using a sensory method, a mechanical rigorometer and low-frequency vibrations. The fish were classified according to rigor state using a neural network. Assessments were done with fish subjected to two different levels of handling stress prior to slaughter. White muscle high-energy phosphates and inosine monophosphate were used as indices of stress showing that handling stress may be considerable during commercial slaughter. Results indicate rigor assessment should be nondestructive and classificaton of rigor state was possible using a lowfrequency vibration method combined with a neural network.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This was a multicentre, double-blind, randomized group comparative study in which 77 children, aged 6–16 years, received 2% nedocromil sodium eye drops and 72 received placebo, one drop into each eye twice daily. The treatment period was 4 weeks, covering the peak birch pollen season. Prior to the start or the season, patients who had mended the clinic the previous 2 years because of seasonal allergic conjunctivitis (SAC) to birch pollen, entered a one week baseline period during which symptoms were assessed, diary cards completed, and routine sampling of blood and urine earned out. The double-blind treatment period then commenced at the onset of the birch pollen season. Patients parents kept daily diary record cards of eye symptom severity and concomitant therapy. Conjunctivitis was mild in both treatment groups but nedocromil sodium was more effective than placebo in controlling symptoms. During the 2–3 weeks of peak pollen counts, this therapeutic effect was statistically significant for itching (P 〈0–01), watering (P 〈0.05) and total symptom score (P 〈0.01), but was not significant for grittiness (P= 0.08) or redness (P = 0.06). Global opinions of efficacy showed no difference between treatments, due to a high placebo effect (however, the diary card data indicated a significant improvement with nedocromil sodium). We therefore conclude that nedocromil sodium 2% eye drops, administered twice daily, is an effective treatment for SAC in children.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The intracellular pathway of a endocytosed glycoprotein has been studied in rainbow trout (Oncorhynchus mykiss) liver by means of differential and isopycnic gradient centrifugation. When 125I-tyramine-cellobiose-labelled asialoorosomucoid was injected intravenously, the protein was removed rapidly from the blood by hepatic endocytosis. The tracer was localized initially in a small, slowly sedimenting organelle (endosome) and transferred to the denser lysosomes where degradation took place. This process took several hours at the acclimated temperature of 10 ° C. The transport could be accelerated markedly or retarded by transfer of fish to higher or lower temperatures respectively. The intracellular transport steps were more susceptible to changing temperature than the internalization process. In particular, the degradation was temperature sensitive. These results may have implications for the effects of changing temperature on immune function in fishes. The impairment of transport upon a rapid temperature decrease, will reduce the rate of antigen processing and presentation in these animals.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The catabolic fate of circulating collagen (Col) in the Atlantic salmon (Salmo salar) was studied. Serum t1/2 and organ distribution of circulating Col in salmon were determined using Col conjugated with l25I-tyramine cellobiose (125I-TC), a low molecular weight adduct which is trapped intralysosomally at the site of uptake. Intravenously administered l25I-tyramine cellobioselabelled Col type I was prepared either from salmon skin (sCol) or rat skin (rCol). Biphasic clearance kinetics of l25I-TC-sCol in salmon were apparent, with 78% being removed from the circulation in an initial rapid α-phase (t1/2(α) = 2.4 min), and 22% being removed more slowly in a terminalβ-phase (t]2(β) = 25.8 min). Serum half life of 125I-TC-rCol was found to be 5.4 min (in this type of experiment the number of data points allow the determination of only a monophasic decay slope). Approximately 90% of recovered radioactivity was found in the kidney of the fish. In comparative experiments, 74% of administered 125I-TC-sCol was cleared from the circulation of rats during an initial rapid α-phase with tl/2(α) = 0.8 min, and 26% was eliminated in the terminal β-phase with t1/2(β) = 7.2 min l25I-sCol was endocytosed and degraded in pure cultures of ral liver endothelial cells, which are the main site of clearance of circulating Col in the rat. Moreover, Col from the two species competed for the same receptor on cultured rat liver endothelial cells, Intravenous administration of tetramethyl rhodamine isothiocyanate-labelled sCol (TRITC-sCol) in salmon, and subsequent examination of sections of kidney in the fluorescence microscope, revealed that the fluorochrome was accumulated exclusively in discrete vesicles of sinusoidal lining cells. Analyses of kidney tissue 24h after intravenous administration of a mixture of fluorescein isothiocyanate-labelled latex beads and TRITC-sCol revealed no codistribution of the two fluorochromes, suggesting that the injected Col was taken up in cells different from macrophages. Purified pronephros macrophages prepared after simultaneous injections of stained beads and Col contained only fluorescein-labelled latex particles. Interestingly, the cells which had accumulated TRITC-sCol appeared to be equally distributed in both pronephros and the part of the kidney containing tubuli. We conclude that Col which gains access to the circulation of the Atlantic salmon is cleared mainly by uptake into sinusoidal lining cells of the kidney. These cells are distinct from phagocytosing macrophages, and morphologically similar to the highly specialized scavenging endothelial cells of mammalian liver sinusoids.
    Type of Medium: Electronic Resource
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