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  • 1990-1994  (3)
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  • 1
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A cross-reactive monoclonal antibody (mAb), designated 1205, was used to study redistribution, parasitophorous vacuole (PV) incorporation, and in situ antigen production during the intracellular parasite development ofEimeria acervulina andE. tenella. Western-blot analysis of sporozoite preparations showed that the mAb recognized antigenic bands at 55 and 80 kDa. Indirect immunofluorescent antibody (IFA) labeling of sporozoites produced an internal dot pattern. Immunogold electron microscopy (IM) showed labeling of dense granules within sporozoites. The IFA pattern changed to a general-internal label in immature schizonts followed by a surface-tip pattern in mature merozoites both in vitro and in vivo. IM of the asexual stages revealed the same labeling pattern for the in vivo development of both species, and labeling of rhoptries was seen. In vitro, the PV membrane together with amorphous material within the PV was labeled by IFA during schizont development forE. tenella. No IM labeling of either the PV membrane or material within the PV was observed. Sexual stages seen in vivo for both species had the general-internal IFA pattern.
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  • 2
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The antigens recognized by hyperimmune rabbit serum raised against tachyzoites of the NC-1 isolate ofNeospora caninum were characterized using chemiluminescent Western blotting, immunogold-silver staining and immunoelectron microscopy. Approximately 20 immunodominant antigens whose relative rates of migration were 16–80 kDa were recognized by the serum in Western blots using reduced or nonreduced parasite antigen preparations. The nonreduced parasite antigens were more strongly recognized by the serum than were the reduced antigen preparations. Immunoelectron microscopy revealed that the rabbit-serum-labeled antigens were localized to some organelles ofN. caninum tachyzoites and to the parasitophorous vacuole surrounding them. In particular, antigens found in the dense granules, in the micronemes, and in the posterior portion of the rhoptries were strongly labeled by an indirect immunogold-labeling technique
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  • 3
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eimeria spp. from the domestic fowl were examined for genetic relatedness by the random amplified polymorphic DNA (RAPD) assay. Nine different oligonucleotide decamers with arbitrary DNA sequences were tested as primers to amplify DNA from sixEimeria species infecting chickens. Two strains each ofE. acervulina andE. tenella were used. Depending on the species/strain-primer combination, between 1 and 12 DNA segments ranging in size from 0.16 to 4.95 kb were amplified. The two strains ofE. acervulina showed minor and major differences in their amplified DNA patterns, giving a similarity coefficient of 61%. The two strains ofE. tenella seemed to be more closely related, yielding a similarity coefficient of 98%. The differences observed between species were greater than those found between strains with every primer used, indicating that the RADP assay could be a useful tool for the study of relationships among these coccidia. The results obtained in this study also indicate the presence of unique, species-specific, amplified DNA segments that could be exploited to identifyEimeria species of the chicken.
    Type of Medium: Electronic Resource
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