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  • 1
    ISSN: 1432-0983
    Keywords: Yeast ; Glycerol kinase ; GUT1 ; ADR1 control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The GUT1 gene of Saccharomyces cerevisiae, encoding glycerol kinase, was cloned and sequenced. The cloned genomic DNA fragment contains an open reading frame potentially coding for a protein of 709 amino acids with homology to bacterial glycerol kinases (40.8% identity over 502 amino acids, and 42.1% identity over 496 amino acids, in comparison to the smaller E. coli and B. subtilis enzymes). Disruption of GUT1 showed that the gene is required for growth on glycerol, but not on glucose or ethanol media. No glycerol kinase activity was detected in the disruption mutant. According to enzyme activity and transcript analysis, synthesis of glycerol kinase is repressed by glucose, and derepression is ADR1-dependent.
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  • 2
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A sample of 114 primary breast tumors and corresponding constitutional DNA were tested for loss of heterozygosity (LOH) of the YNZ22 and p53 genes, both located in the 17p13 region. Loss of the p53 allele was found in 28 of 44 primary breast carcinomas (64%). In contrast LOH in only 26 of 61 tumors (43%) was detected with the variable number of tandem repeats (VNTR) probe YNZ22 mapping at 17p13.3 close to the p53 locus at 17p13.1. Among 19 tumors informative for both probes allele loss at 17p13.3 never occurred without p53 involvement. These data suggest, that p53 is the target of 17p13 allelic deletions in human breast cancer. Immunohistochemistry showed overexpression of the p53 protein in 25 of 50 cases (50%) presumably reflecting activating point mutations. Overexpression was not correlated with allele loss but seemed to be closely related to the presence of point mutations in this study. No homozygous deletions or rearrangements of the p53 gene were detected. This would argue for an important role of heterozygous p53 mutations in human breast cancer.
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  • 3
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract cDNA probe of the casein kinase 2α subunit gene detects a biallelic PstI polymorphism. This restriction fragment length polymorphism is the first known genetic marker of this gene.
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  • 4
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Nous avons eu recours, pour allonger les membres inférieurs, à la méthode d'Ilizarov de distraction épiphysaire depuis 1977 et à sa nouvelle technique de corticotomie ou compactotomie depuis 1983. La première méthode a été utilisée pour 22 membres inférieurs avec un allongement moyen de 8.25 cm (de 4 à 18 cm). Sont inclus dans ce groupe deux achondroplases chez qui les deux membres inférieurs ont été allongés de 12 cm. Dans la deuxième méthode on pratique une corticotomie au niveau de la jonction dia-métaphysaire, suivie d'une distraction avec l'appareil d'Ilizarov (4×0.25 mm par jour). Nous avons allongé 30 membres inférieurs, parmi lesquels 5 patients qui ont eu un allongement bilatéral compris entre 8 et 12 cm. Chez les autres on a obtenu un allongement moyen de 7.9 cm (de 4 à 15 cm). Le rapport entre la durée du traitement par cm obtenu (c'est à dire le temps écoulé entre l'opération et la mise en charge complète divisé par le nombre de cm d'allongement) est plus faible après distraction epiphysaire qu'après corticotomie. Les complications sont également un peu moins fréquentes avec cette dernière technique. La distraction épiphysaire doit être réservée aux enfants, de l'âge de 12 ans jusqu'à la fin de la croissance. L'avantage de la corticotomie est qu'elle peut être effectuée à n'importe quel âge, de 5 à 30 ans. Dans les deux cas, il faut prendre le plus grand soin pour éviter les complications.
    Notes: Summary We have used Ilazorov's method of distraction epiphyseolysis for leg lengthening since 1977 and his new method of corticotomy or compactotomy since 1983. The first method was carried out in 22 lower limbs with an average lengthening of 8.25 cm (range 4 to 18 cm); included in this group are 2 patients with achondroplasia in whom both legs were lengthened 12 cm. In the second method, a corticotomy is carried out at the metaphyseal-diaphyseal junction followed by distraction in Ilazorov's apparatus (4×0.25 mm a day). We lengthened 30 legs; included are 5 patients who had both legs lengthened from between 8 and 12 cm. In the remainder an average of 7.9 cm of lengthening was achieved (range from 4 to 15 cm). The index for the treatment time per cm achieved (time from operation until full weight-bearing per cm of lengthening) was shorter after distraction epiphyseolysis than after corticotomy. Complications were also a little less after the latter procedure. Distraction epiphyseolysis has to be limited to children from aged 12 years until growth ceases. The advantage with corticotomy is that it can be done at any age from 5 to 30 years. Care is needed in both cases to avoid complications.
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  • 5
    ISSN: 1432-2250
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract There has been substantial effort recently put into proving, for a variety of different geometries, the existence of trapped waves, that is unforced time-harmonic motions which do not radiate energy to large distances. Thus it is known that such motions can exist in a deep channel which includes a cylinder spanning the channel, for various shapes of cylinder. The converse problem of proving the absence of such trapped waves has received much less consideration, and the only relevant uniqueness proof for a channel spanned by a cylinder is that of McIver (1991). In an appendix to that paper, McIver demonstrates that no trapped-wave motions can exist for the case in which the cylinder is surface piercing and is entirely contained between vertical planes through the free-surface intersections. This is exactly the same geometrical condition which John (1950) found would ensure uniqueness in water-wave radiation and scattering problems, in finite or infinite depth. Both John and McIver achieved their uniqueness results by consideration of integrals of the potential along vertical lines down from the free surface. John's work was extended by Simon and Ursell (1984) who established uniqueness for a wider class of two-dimensional radiation and scattering problems by consideration of integrals along nonvertical lines. The work presented in this paper is the corresponding extension of McIver's work; although this extension does not rule out trapped waves at all frequencies for any geometry except that already considered by McIver, it does yield an easy lower bound for the ratio of the trapped-mode frequency to the cut-off frequency, in finite or infinite depth.
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  • 6
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le diagnostic non invasif d'hémangiome caverneux du foie est une étape importante dans l'investigation des patients ayant des lésions hépatiques focales, puisque la biopsie risque de se résoudre par une hémorrhagie menaçant le pronostic vital. Pour définir la précision diagnostique de la tomoscintigraphie après marquage des hématies au technetium 99m (99mTc), on a étudié 50 patients ayant des types différents de lésions focales du foie. Trente-deux patients avaient 45 hémangiomes et 7 lésions bénignes tandis que 18 patients avaient des tumeurs hépatiques malignes soit primaires (n=8) soit secondaires (n=10). L'imagerie tomographique a identifié 12 hémangiomes de plus que l'imagerie planaire, améliorant la sensibilité de 53% à 80% et s'est avérée très utile pour détecter les lésions plus petites (taille moyenne, 2.1 cm). La spécificité pour les hémangiomes était de 100% pour toutes les lésions dépassant 1.9 cm montrant les caractères scintigraphiques typiques d'accumulation de sang sur les images retardées. Il y a eu concordance totale entre 2 observateurs indépendents sur l'interprétation des images tomographiques (89% pour le groupe des hémangiomes et 100% pour les autres patients). Voilà pourquoi la scintigraphie99mTc avec tomographie est une technique de diagnostic précis dans l'investigation des hémangiomes caverneux. Sa valeur essentielle est sa capacité de distinguer les hémangiomes des autres types de pathologie hépatique.
    Abstract: Resumen El diagnóstico no invasivo del hemangioma cavernoso del hígado es un paso de importancia en el proceso de investigación de pacientes con lesiones hepáticas focales, puesto que la biopsia puede resultar en hemorragia de consecuencias potencialmente fatales. Con el objeto de determinar la certeza diagnóstica de la tomografía con glóbulos rojos marcados con tecnecio 99m, se estudiaron 50 pacientes con varios tipos de lesiones hepáticas focales. Treinta y dos pacientes presentaron 45 hemangiomas y 7 lesiones benignas, en tanto que 18 pacientes presentaron neoplasias malignas primarias (n=8) o secundarias (n=10). Las imágenes tomográficas obtenidas por este método lograron identificar 12 hemangiomas más, en comparación con imágenes convencionales, aumentando la sensibilidad de 53% a 80%, y demostró ser de máxima utilidad para la detección de las lesiones más peque¯nas (tamaño promedio, 2.1 cm). La especificidad para los hemangiomas fue 100%, con todas las lesiones mayores de 1.9 cm exhibiendo el patrón centelleográfico característico de estancamiento sanguíneo en las imágenes tardías. Se encontró excelente correlación entre 2 observadores independientes en la interpretación de las imágenes tomográficas (89% para el grupo de pacientes con hemangiomas y 100% para el resto de los pacientes). En consecuencia, la escintigrafía con glóbulos rojos marcados con99mTc aparece como una técnica certera en el diagnóstico de los hemangiomas cavernosos. Su mayor valor reside en su habilidad para diferenciar los hemangiomas de otra clase de patología.
    Notes: Abstract Noninvasive diagnosis of cavernous hemangioma of the liver is an important step in the investigation of patients with focal hepatic lesions since biopsy may result in life-threatening hemorrhage. To determine the diagnostic accuracy of 99m-technetium red blood cell (99mTc-RBC) imaging with tomography, 50 patients with various types of focal liver lesions were studied. Thirty-two patients had 45 hemangiomas and 7 other benign lesions while 18 patients had either primary (n=8) or secondary (n=10) hepatic malignancies. Tomographic imaging identified 12 more hemangiomas than planar imaging, improving sensitivity from 53% to 80%, and was found most useful for detection of smaller lesions (mean size, 2.1 cm). Specificity for hemangiomas was 100% with all lesions greater than 1.9 cm showing the characteristic scintigraphic pattern of blood-pooling on delayed images. There was excellent agreement between 2 independent observers concerning interpretation of tomographic images (89% for the hemangioma group and 100% for other patients). Therefore99mTc-RBC scintigraphy with tomography is an accurate diagnostic technique in the investigation of cavernous hemangiomas. Its major value resides in its ability to distinguish hemangiomas from other types of hepatic pathology.
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  • 7
    ISSN: 1432-069X
    Keywords: ICAM-1 ; Etretinate ; Lichen planus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
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  • 8
    ISSN: 1432-0584
    Keywords: Autoimmune thrombocytopenia ; Immunosuppressive therapy ; Vinblastine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Forty-three adult patients with idiopathic thrombocytopenic purpura (ITP) were treated by slow intravenous infusions of vinblastine. Nineteen had ITP of recent onset (i.e. of less than 6 months duration) and had contraindication to steroids (3 patients), refractoriness to steroids (6 patients) or to steroids and high dose intravenous immunoglobulins (IVIg, 10 patients). Of the 19 patients, 10 achieved complete response (CR), 2 achieved partial response (PR), 2 had minor response (MR) and the remaining 5 patients had no response (NR). Six of the complete responders remained in CR after 12 to 48 months, whereas all other responders relapsed within 3 months, in spite of maintenance therapy. Twenty-four patients had chronic ITP (i.e. of 6 months duration or more) and had showed no or only transient response to steroids and/or splenectomy, and in many of them, to other therapeutic approaches. Four achieved CR, 4 PR, 6 MR and 10 NR. All but 2 responses were shorter than 3 months, in spite of maintenance therapy. Most responses to slow infusions of vinblastine began after the first infusion. Main side effects included leukopenia in 9 patients (but with absolute neutropenia in only one) and peripheral neuropathy in 2 patients. Interval from diagnosis was the only prognostic factor of response to treatment. We conclude that slow infusions of vinblastine may be a useful approach in ITP of recent onset, when contraindication or refractoriness to steroids and/or IVIg exists. In our experience, this treatment has limited benefit in chronic ITP. In addition, it remains to be demonstrated that slow infusions of vinca alkaloids have any superiority over intravenous bolus injections of the same drugs.
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  • 9
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cellobiose oxidase from Phanerochaete chrysosporium was used for continuous monitoring of cellulase action on microcrystalline cellulose (Avicel). Two protocols are described, the parameter monitored being either the decline in electrode potential as ferricyanide is reduced or consumption of dioxygen. Most experiments used a commercial cellulase preparation from Trichoderma reesei and ferricyanide as acceptor. Within 1 min of an addition of cellulase, ferricyanide reduction reached a steady rate. This was converted into a rate of production of substrate for celobiose oxidase, in μmol·min−1. Experiments were conducted either with a constant concentration of cellulase and increasing Avicel, or with constant Avicel and increasing cellulase. Kinetic analysis of the experiments with constant cellulase indicated a K mof 4.8 ± 1.0 (g cellulose)·1−1, which was close to the value predicted from binding studies. The specific activity of the cellulase was measured as 375±25 μmol·(g cellulase)−1·min−1 in experiments with a high cellulose concentration, but was less than half this value when the cellulose was saturated with cellulase. The maximal rate of cellulose degradation was 9.6±1.3 μmol·(g cellulose)−1·min−1.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 52 (1991), S. 361-366 
    ISSN: 1432-0649
    Keywords: 51.70 ; 82.40P
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract It will be shown, how the spatial decay of active molecules — excited electronically or activated otherwise (e.g., chemical processes) is determined by diffusion and flow as well as by the intrinsic molecular constant. The exact analysis in cylindric geometry leads to a set of unique solutions, which in lowest order are characterized by a weak gradient of the particle concentration at the wall and a pronounced kernel in the center of the tube. In special cases there exists a simple but powerfull expansion of any distribution in the source, which are very similar to Fourier-Bessel series. The solutions in lowest order will be used to analyze the measured decay of activated oxygen in a steady stream of molecular oxygen. Since the decay constant is determined by volume deactivation and by the removal of particles at the wall, evaluation of experimental data, however, remains necessarily ambiguous.
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