Biochemistry and Biotechnology
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
The effects of concentration on immunoglobulin G (IgG) oligoclonal bands after isoelectric focusing in cerebrospinal fluids (CSF) were examined using six concentration procedures, and the percent recoveries of IgG varied. The procedures, % IgG recoveries, and corresponding degrees of concentration are as follows: (1) Amicon CF25 membrane cone, 75 %, 45 ×; (2) Amicon CF50A membrane cone, 65 %, 3 ×; (3) Amicon CS15 Minicon, 47 %, 80 ×; (4) Microprodicon, 55 %, 35 ×; (5) collodion bag negative pressure dialysis, 50 %, 16 ×; and (6) microcollodion bag in Permasorb, 40-80 %, 10-60 ×. Ultracentrifugation of CSF at 100 000 g for 1 h prior to concentration using CF 25 membrane cones introduced not more than 5 % variation in the IgG concentrations. Isoelectric focusing was performed on 20 CSF samples concentrated by CF25 membrane cone, CS15 Minicon, and collodion bag, and no effects on the oligoclonal IgG banding patterns were found.
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