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  • 1
    ISSN: 1432-072X
    Keywords: Streptococcus mutans ; Glucosyltransferase ; Fructosyltransferase ; Electrophoresis ; Isoelectric focussing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Streptococcus mutans was classified by the electrophoretic properties of glucosyltransferases (GTases) and fructosyltransferases (FTases). The cells of serotypes a, d and g did not release extracellular FTases, although those from other serotypes did. The enzymes from cells of serotypes d and g synthesized a good deal of insoluble polysaccharide compared with other serotypes. The enzymes were applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and polyacrylamide gel-isoelectric focussing (PAG-IEF). Gels were stained for their activity and protein content. Enzymes belonging to the same serotype gave the same specific pattern on both gels. The seven serotypes could be classified into the following four groups: serotypes d and g, serotype a, serotypes c, e and f, and serotype b. The results agree well with some previous reports based on other methods. The molecular weights of three GTase bands were 156K, 146K and 135K, and of four kinds of FTase bands were 108K, 95K, 80K and 76K. The isoelectric points of main enzymes were 4.25, 4.60, 5.00, 5.55 and 5.70. Those of FTases were 4.25 and 4.60.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Chemotaxis ; Information processing ; Oscillation ; Physarum polycephalum ; Thermal image camera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The spatial and temporal pattern of oscillating temperatures on the cell surface of a plasmodial strand ofPhysarum polycephalum was measured with a sensitive thermal image camera. The longitudinal tension of the strand was studied simultaneously. In the absence of chemical stimulation, the phases of the temperature oscillation observed at various portions of the strand were entrained with almost coincidental phase. The temperature and tension oscillation were synchronized, although the phase difference between them was occasionally changed. With local chemical stimulation, the phase of the temperature oscillation advanced in the portion to which the plasmodium would be induced to migrate. The phases between temperature and tension oscillations then became constant. The mechanism by which the plasmodium processes local information of chemical stimulus to global information for the migration is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 62 (1986), S. 53-59 
    ISSN: 1573-2622
    Keywords: diagnostic power ; fixation ; optic nerve disease ; pattern ERG ; pattern VER
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We analyzed the electroretinogram elicited by pattern stimuli (PERG) in 29 patients with unilateral optic nerve disease. The normal fellow eyes served as a control. When the normal fellow eye was covered and the patient was asked to watch the center of the pattern stimuli with the affected eye, the poor fixation often caused irregular eye movements which resulted in a poor reproducibility of the response. In such instance we opened the normal fellow eye for fixation, which helped to stabilize the affected eye being examined even when visual acuity was extremely poor. The cross-talk phenomenon of the ERG and the reflection of the VER from the normal fellow eye were proved negligible in this recording condition. We analyzed PERGs in comparison with the visual acuity and the degree of optic atrophy. Unlike the results of many previous authors, little correlation was observed between PERG and these two factors. Most patient showed normal or only slightly abnormal PERG, even when the visual acuity was extremely poor and the optic disc was completely atrophic. Our results suggest that, at least with our method, PERG has extremely limited value in detection of optic nerve disease.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1572-9567
    Keywords: critical phenomena ; cyclohexane ; polystyrene ; shear modulus ; ultrasonics ; viscosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Ultrasonic shear measurements were conducted on polystyrene-cyclohexane solutions at 3, 51, and 252 kHz using the crystal fork and torsion methods. The real and imaginary parts of the complex shear modulus above the critical point are compared with modified theoretical expressions derived within the framework of the decoupled-mode theory. For this comparison, a background part was assumed to be described by a scaling form proposed by de Gennes. Numerical analysis of the data shows a satisfactory agreement between the theory and the experiments for ultrasonic shear data over a wide range of reduced frequencyω ... In addition, it is shown that the description of the simple viscosity dynamical scaling function is broken at a high-frequency limit.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Complicated retinal detachments in 99 eyes were successfully managed by intraocular injections of silicone oil with or without pars plana vitrectomy. The silicone was then subsequently removed after panretinal photocoagulation. Any remaining anterior vitreous cortex near the vitreous base and/or preretinal proliferative tissue causing traction of the retina was also removed by a vitrectome or extracted by a hooked needle after the silicone-fluid exchange had been performed. The preretinal proliferation occurred in some cases even after silicone oil had been removed. Recurrent detachments were discovered in 8 of the 99 eyes and were managed successfully by reinjections of the silicone. Other silicone reinjections were required in two eyes with diabetic retinopathy and two nondiabetic eyes because of frequent rebleeding into the vitreous cavity.
    Type of Medium: Electronic Resource
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