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  • 1
    Call number: ZZ1990:3
    Keywords: DKFZ-publications
    Pages: 33 p.
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    ZZ1990:3 available
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  • 2
    Call number: ZZ1982:10/2(2)
    Keywords: DKFZ-publications ; DKFZ-publications
    Notes: Mitarbeiter: Ulrich Abel, Jutta Berger, Lutz Edler, Armin Koch, Renate Rausch.
    Pages: 91 p.
    Edition: [2. Aufl.] IBM PC/AT - Version 1
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  • 3
    Call number: ZZ1985:16
    Keywords: DKFZ-publications
    Pages: 34 p.
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    ZZ1985:16 available
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  • 4
    ISSN: 1432-2307
    Keywords: Insulin ; Insulinomas ; Ultrastructure ; Immunogold technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Localisation of insulin-like immunoreactivity has been studied using the immunogold staining procedure on thin sections of 6 human insulinomas, conventionally processed for electron microscopy. The labelling was restricted to the secretory granules. Depending on their morphology, these either resembled B-cell granules of human adult pancreas or belonged to the atypical (non-diagnostic) group. Within the former group, those with a crystalloid core or an amorphous dense or moderately dense core were strongly immunoreactive, whereas others, filled with a pale material, were poorly labelled. Most granules of this type were stored together within the heavily granulated cells of 3 insulinomas, presenting the classical features of clinical and biological behaviour and a typical light microscopic staining pattern. In contrast, the non-diagnostic granules, characterized by their smaller size, a very dense core and a thin halo, were mainly found within the poorly granulated cells making up the other tumours, and showed a very uneven labelling. Strongly labelled granules were found in one insulinoma that also belonged to the classical type; these were stored together with a few diagnostic granules within the same cells. Only poorly labelled atypical granules were present in two cases revealing a number of unusual features; these included moderate elevation of insulinaemia, uncertain tumour histology, as well as weak immunostaining for insulin/proinsulin and variable argyrophilia of the tumour in paraffin sections. These findings suggest that human insulinomas differ not only in storage capacity but also in their degree of granule maturation. This may involve some deficiency of either the prohormone conversion or the subsequent processing of the cleavage products.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2307
    Keywords: Gastrin ; Gastrinomas ; Ultrastructure ; Immunogold technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Localisation of C-terminal gastrin immunoreactivity has been studied, using the immunogold staining procedure, on ultrathin sections of 6 human gastrinomas conventionally processed for electron microscopy. The specific labelling, whose density depended on the mean diameter of the gold marker, was restricted to endocrine secretory granules. However, in poorly differentiated cells from malignant tumours, a number of granules remained unreactive. The labelling pattern depended also on the functional state of each cell. The immunoreactive granules showed various morphological features. A moderate number of gold particles was demonstrated over the floccular content of the infrequent diagnostic G-type granules. Non-diagnostic round granules of varying size and electron density were prevalent in most cells; their usually strong immunostaining allowed immediate recognition of cell specificity. Dense granules which were large in size and angular in shape and present in one case, were also intensely labelled. In the same tumour, unequal labelling occurred over polymorphous, often elongated granules, of varying size. Granules of different types, including intermediate forms, could be found in the same cell, indicating a spectrum of granule maturation towards well-defined types of the fetal or adult normal tissues. The present methodology would help to identify gastrin-producing cells in prospective or retrospective electron microscopy studies of multihormonal endocrine tumours.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2307
    Keywords: Medullary thyroid carcinoma ; Calcitonin ; Amyloid fibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using 3 polyclonal antisera directed against synthetic human calcitonin, we investigated at the electron microscope level the intra-or-extracellular fibrillar/filamentous aggregates found in 4 amyloid-rich medullary thyroid carcinomas (MTC) and in a number of other endocrine polypeptide tumours with or without demonstrable amyloid deposition. The antisera were applied by the immunogold procedure on ultrathin sections of glutaraldehyde-fixed, usually osmium-postfixed, tissues. In MTC cases, a strong labelling was present over two types of aggregates: one composed of rigid, criss-crossing fibrils 7–10 nm in diameter, suggestive of amyloid, and the other consisting of loosely arranged fibrils, 4–7 nm in width, often wavy or poorly defined. In both cases, the labelling was closely associated with that part of the sectioned fibril exposed to the antiserum. Amorphous material was sometimes present adjacent to the latter aggregates, but did not bind the calcitonin antibodies. In contrast, no labelling occurred over the amyloid deposits found in two non-calcitonin-producing endocrine tumours of the pancreas, nor over the cytoskeletal filaments stored in various endocrine polypeptide tumours. The specific value of the labelling for calcitonin-like immunoreactivity was assessed by control tests, such as absorption of the antiserum by excess calcitonin and comparative use of normal serum and antisera directed against human IgG and P component. No immunoreactivity of the MTC amyloid fibrils was found using antibodies directed against katacalcin and human prealbumin. We conclude that in tumour tissues conventionally processed for electron microscopy, MTC amyloid fibrils of varying morphology can be selectively and specifically labelled for calcitonin-like immunoreactivity.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2307
    Keywords: Parathyroid ; Storage granule ; Parathormone ; Chromogranine A ; Immunoelectron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Parathyroid hormone (PTH) and chromogranin A/secretory protein-I (SP-I) have been localized on immunoelectron microscopy in double-fixed tissues from adenomatous and secondary hyperplastic parathyroid glands. Storage organelles, identified on the basis of their consistent labelling, included two morphologically distinct varieties of granules/vesicles; the mature granules and the progranules. The former consisted of dense, mostly rounded, medium to large-sized bodies which were strongly labelled and predominant in the proximity of the cell membrane. The other variety of body included a spectrum of small pale vesicles/granules which were mainly located in the Golgi area. Because their morphology and their labelling pattern varied other bodies were assumed to be engaged in degradation or cleavage of the secretory proteins. These bodies comprised crinophagic structures, that is to say multivesicular bodies and large Golgi-related vesicles, as well as a number of atypical solid bodies. Whereas most of the granulated cells stored a mature or a maturing population of vesicles/granules, the process of maturation appeared to be either absent or incomplete in a number of cells from some glands. The major defects were frequently associated with an unusual labelling pattern of the Golgi area and selectively affected groups of cells from all the transitional oxyphil cell adenomas. The minor defects concerned individual cells of different types present in both categories of glands. The present data suggest that in hyperfunctioning glands, the type of hormone processing depends on the capacity of each cell in progranule maturation and that the maturation capacity may decrease dramatically in adenomatous or chronically hyperstimulated cells of the transitional oxyphil type.
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  • 8
    ISSN: 1432-0983
    Keywords: Plastome ; cpDNA variability ; Dasycladaceae ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cells from Acetabularia mediterranea and Batophora oerstedii have been cloned and several lines established from these clones. Chloroplast DNA (cpDNA) was isolated from these lines and characterized by restriction enzyme analysis. A high degree of restriction fragment length polymorphism was observed between the different lines of each species. This high intraspecific variability in the cpDNA arrangement was confirmed by hybridization to well-characterized chloroplast genes. Based on results obtained from DNA-denaturing experiments, a GC content of 49.9% and 51.7% was calculated for different lines of A. mediterranea and B. oerstedii, respectively. This is close to that found in the cyanobacteria.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1106
    Keywords: Stance perturbation ; Cerebral potential ; Leg muscle e.m.g. response ; Spinal reflexes ; Motor control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to investigate the neuronal mechanisms underlying the compensatory movements following stance disturbance, leg muscle e.m.g. responses and cerebral potentials evoked by a treadmill acceleration impulse were analysed. It was found that the displacement was followed by a cerebral potential of a latency of 40–45 ms and EMG responses in the calf muscles at a latency of 65–70 ms. The e.m.g. responses represented specific compensatory reactions to the mode of perturbation (with a gastrocnemius activation following positive acceleration but a tibialis ant. activation following negative acceleration). The cerebral potentials, however, showed a common pattern to both conditions. In addition, the leg muscle e.m.g. reactions were not altered by learning effects and by forewarning of displacement onset, while the amplitude of the cerebral potentials was significantly smaller in these conditions compared to those produced in response to randomly induced perturbations. It was therefore concluded that the leg muscle e.m.g. reactions are mediated by a polysynaptic spinal reflex pathway which depends on a supraspinal control. The cerebral potentials seem to represent afferent signals which can be supposed to be subjected to modification and processing by supraspinal motor centres, according to the actual requirements.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1041
    Keywords: metronidazole ; clindamycin ; appendicitis ; drug metabolism ; children ; tissue level ; peritoneal fluid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Thirty-one children underwent appendectomy following administration of a single i.v. dose of clindamycin 10 mg/kg or metronidazole 7.5 mg/kg. The serum levels of the antibiotics at that time were comparable, but the tissue concentration of clindamycin in the appendix (mean 7.23 µg/g) exceeded that of metronidazole (mean 2.27 µg/g). The concurrent mean concentration of metronidazole in peritoneal fluid (14.26 µg/ml) was higher than that of clindamycin (7.72 µg/ml).
    Type of Medium: Electronic Resource
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