Springer Online Journal Archives 1860-2000
Summary Using MS medium supplemented with 6% sucrose and hormones, plantlets were regenerated from the expiants of mature seeds and young panicles of IRs and IR54. Out of 157 regenerated plants (R1), three were found to be male sterile (ms): one from IRs and two from IR54, including a fertile and sterile chimaera. In the second generations (R2) of IR24 and IR54, one line from each segregated into male sterile and fertile plants. These ms plants could be divided into two types with pollen failure: pollen free (without pollen) and pollen abortive. IR24 was a semi-restorer for ms-plants of the pollen free type derived from the second generation of IR54 somaclones. The segregation ratio of fertile: sterile in both R2 of line 91 and the F2 of ms-plant/IR24 fitted the formula 15/16∶1/16 quite well, showing that the male-sterile was controlled by two independent nuclear genes. Until now, as we know, male-sterile could be produced by hybridization or mutagenesis: sometimes it could be found in nature by spontaneous mutation. Recently the cytoplasmic male-sterile of tobacco was produced by protoplast fusion. This is the first paper to report male-steriles in regenerated plants and their offspring obtained from somatic cell culture.
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