Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

  • 1985-1989  (1)
  • 1980-1984  (1)
  • 1
    ISSN: 1432-0428
    Keywords: Diabetic control ; urine ; glycopeptides ; affinity chromatography ; non-enzymatic glycosylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relationship between improvement in diabetic control and changes in levels of glycosylated urinary peptides was investigated. Eight poorly controlled Type 1 (insulin-dependent) diabetic patients were studied as optimal metabolic control was achieved. Mean daily blood glucose values and weekly haemoglobin A1 levels were determined simultaneously. Urinary glycosylated peptide levels fell 50% in 15 days, compared with 23 days for haemoglobin A1. Levels of glycosylated urinary peptides were sensitive to increased mean blood glucose concentrations of 9.72 mmol/l and increased linearly up to 20.0 mmol/l (r=0.98) when compared with mean blood glucose levels obtained 8–9 days earlier. A similar correlation was found with haemoglobin A1 levels. Levels of glycosylated urinary peptides before and after optimal control were compared, and a decrease of 40% was observed (pre-control: 269±44 μmol/day, optimal control: 162±45 μmol/day, mean±SEM). The lag time between the fall in mean blood glucose level and the parallel fall in glycosylated urinary peptides was 8–9 days, suggesting that measurement of these compounds may become a useful clinical laboratory technique for monitoring short-term integrated glycaemia in diabetic patients.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The secretions of interleukin 1 (IL-1), tumour necrosis factor α (TNF), and prostaglandin E2 (PGE2) of low-dose E. coli lipopolysaccharide (LPSJ-stimulated human monocytes (Mø) were investigated in an endotoxin (ET)-free milieu (〈1.6 pg LPS/ml). Human Mø cultures from nine healthy men were stimulated with 0, 12.5–500, and 250,000 pg LPS/ml as measured by a very sensitive Limulus test. The IL-1 activity was tested by the mouse costimulatory thymocyte (LAF) assay, which was thoroughly standardized and characterized (interassay variation 22–24%, intra-assay variation 3–7%). Spontaneous Mø secretions of IL-1, TNF, and PGE2 were negligible, but 12.5 pg LPS/ml significantly stimulated the secretions of these Mø products and the monokine responses to 500 and 250,000 pg LPS/ml were almost in the same range. It was demonstrated that the secretions of IL-1-TNF and TNF-PGE2 were strongly correlated. Pronounced interindividual differences in LPS responsiveness were demonstrated, and two low-responders, one of whom was HLA-DR 1,2-positive, were identified. Three first-degree relatives of the DR1.2-positive low-responder had similar low responses. Furthermore. Mø cultures were prepared weekly for 4 weeks from four HLA-DR different men and the only DR2.2 homozygous individual had low monokine responses. In conclusion, stable interindividual differences in in vitro monokine and PGE2 secretions of LPS-stimulated Mø were demonstrated. It is suggested that HLA-DR2-positive individuals may be low responders.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...