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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 3 (1964), S. 522-524 
    ISSN: 1432-0533
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A method, demonstrating Glomeruli cerebellosi using Victoria Blue 4 R after KMnO4-treatment on Bouin-fixed paraffine preparations is described.
    Notes: Zusammenfassung Eine Methode zur Darstellung der Glomeruli cerebellosi mit Viktoriablau 4 R nach vorheriger Behandlung mit KMnO4 am Bouin-fixierten Paraffinschnitt wird angegeben.
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  • 2
    ISSN: 1612-1112
    Keywords: Aniline ; Dimethylthiophosphinyl derivatives ; Mass fragmentography ; Environmental analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A method for trace analysis of aniline in water, urine and serum samples is presented. Internal standardisation with d5-aniline, extraction, derivatisation with dimethylthiophosphinic chloride, gas chromatographic separation on a glass capillary column and mass fragmentographic quantitation are essential steps of the analytical procedure. A detection limit of 20 pg/ml in water and 500 pg/ml in urine or serum is achieved.
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  • 3
    ISSN: 1612-1112
    Keywords: Ion-pair reversed-phase HPLC ; Protoporphyrin ; Zinc protoporphyrin ; Red blood cells ; Porphyria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An extraction and ion-pair reversed-phase HPLC procedure is described for the separation and determination of protoporphyrin and its zinc chelate from red blood cells. The recoveries were greater than 90% with coefficients of variation between 8 and 10%. A linear response curve was obtained from 800 to 4000 μg/l erythrocytes for the porphyrins.
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 192 (1961), S. 288-289 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] From a selection of X-ray-irradiated progenies of 70,000 r. treatment of the X, generation of Gor-ohorua olitorius (i7.nO 632) in 1960, seven variants were found from a culture of healthy, vigorously growing normal plants. These plants with comparatively slender stom and small leaves were very ...
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 295 (1982), S. 587-589 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The best studied double seismic zone is that beneath Northeast Honshu, Japan (Fig. 1). Note that the double zone of seismicity occurs in a portion of the downgoing plate that appears to be straight. We sought to evaluate whether the thermal stresses that result from the warming of the plate as it ...
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  • 6
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Ion-pair reversed-phase ; Free porphyrin carboxylic acids in urine, complete separation ; Quantitation within 4.4-5.4% precision ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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  • 7
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Das Ektoneuralsystem des Seesternes Asterias rubens L. wurde licht- und elektronenmikroskopisch untersucht. Dabei wurde einmal seiner Cuticula, dann seinen epithelialen Elementen unter dem Gesichtspunkt ihrer etwaigen Zugehörigkeit zur Glia besondere Aufmerksamkeit geschenkt. Folgende Ergebnisse konnten erzielt werden: 1. Wie die übrige Körperdecke wird auch das Ektoneuralsystem von einer isolierbaren Cuticula überzogen. Diese etwa 4 μ dicke Schicht läßt sich färberisch und elektronenmikroskopisch in eine basale und eine oberflächliche Zone differenzieren. Am Aufbau der Cuticula sind Proteine beteiligt. Die elektronenmikroskopische Untersuchung zeigt, daß die Cuticula gleichmäßig von 1300 Å weiten Röhren durchsetzt ist, die in Abständen von 2000 Å stehen. Durch die Porenkanäle ziehen Cytoplasmafäden hindurch, die sich aus den apikalen Abschnitten der subcuticularen Stützzellen erheben. Diese Mikrovilli sind im Basalabschnitt der Porenplatte meistens spiralig gewunden. Sie erreichen die freie Oberfläche des Körpers. In den Mikrovilli liegen ovoide Partikel ungeklärter Natur, wie sie auch in den Perikaryen der Stützzellen vorkommen. Kinocilien, welche die Cuticula durchsetzen, sind bei Asterias nicht beobachtet worden. 2. Die Cuticula wird von einem Filzwerk zarter Filamente unterlagert, die parallel zu ihrer Oberfläche ausgerichtet sind. Ein Teil der Filamente strahlt in die Cuticula ein. 3. Licht- und elektronenmikroskopisch ist eine subcuticulare Zone von lockerer Bauweise zu erkennen. Sie wird durch die Gesamtheit der Mikrovilli gebildet, die sich aus den apikalen Abschnitten der Stützzellen erheben, um in die Porenkanäle der Cuticula einzudringen. 4. Die Stützzellen ziehen in auffallend regelmäßiger Verteilung durch die gesamte Tiefe des Ektoneuralsystems hindurch, d. h. von der Cuticula bis zur sog. Mesodermlamelle. Ihre kernhaltigen, apikalen, mit den erwähnten Mikrovilli ausgestatteten Abschnitte sind untereinander durch ausgedehnte Cytodesmen verbunden, die aus schmalen, senkrecht zum Plasmalemm gerichteten Membranen bestehen. Die Membranen wahren einen regelmäßigen Abstand von 340 A. Diese Art der Zellverbindung dürfte bei Wirbellosen häufiger anzutreffen sein, da sie auch bei Seeigeln, bei Mollusken und bei Planarien vorkommt. 5. Im Cytoplasma der Stüzzellen liegen gröbere Granula, die PAS-positiv sind und Proteine enthalten. 6. Das bezeichnende Merkmal der Stützzellen sind langgestreckte, die Zellleiber von der Cuticula bis zur Mesodermlamelle durchsetzende Fasern mit kreisrundem oder ovalem Querschnitt. Die Stützfasern sind positiv einachsig doppelbrechend und leuchten unter dem Fluoreszenzmikroskop wie Gliafasern auf. Aus elektronenmikroskopischen Aufnahmen geht hervor, daß die Fasern aus dicht gepackten, in Faserlängsrichtung orientierten Filamenten bestehen, die teilweise eine auffallende periodische Querstreifung besitzen. Diese Querstreifung stimmt nicht mit der periodischen Gliederung der Bindegewebsfibrillen bzw. -filamente von Asterias rubens L. überein. 7. Es wird dargelegt, daß Herkunft, cytologische Merkmale und topisches Verhalten der Stützzellen den Kriterien der Gliazellen in so hohem Maße entsprechen, daß man die Stützzellen des Ektoneuralsystems von Asterias rubens L. als Gliazellen bezeichnen darf. Sie lassen sich dem bei Wirbeltieren anzutreffenden Typus von ependymaler Faserglia vergleichen.
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  • 8
    ISSN: 1432-1440
    Keywords: Myopathy ; Carnitine deficiency ; Lymphocytoma ; Muscle biopsy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 47-year-old male patient suffered from recurrent myalgia, induced by fasting or physical exercise. Later, he developed progressive muscular weakness. Serum levels of creatine phosphokinase (CPK) were elevated to approx. 400 U/l. Muscle biopsies showed lipid storage myopathy and signs of acute fiber necrosis, muscle carnitine was decreased to below 20% of controls, carnitine palmitoyl transferase (CPT) activity was normal. Carnitine was also moderately decreased in a liver biopsy and in plasma. Urine excretion of carnitine was low, no elevation of short-chain dicarboxylic acids could be found. The patient was also found to suffer from a lymphocytic malignant non-Hodgkin lymphoma, a monoclonal immunoglobulin (Ig) G-k in plasma and lymphocytic infiltration of bone marrow were demonstrated. At that time no evidence had been obtained to indicate that these two diseases could be related to each other. Autoantibodies against skeletal muscle could not be demonstrated. Absorption of L-carnitine p.o. was normal, however, plasma levels of carnitine fell again rapidly. Administration of 3 × 2 g L-carnitine per day normalized the patient's plasma carnitine levels and led to an increase of plasma short-chain acylcarnitine and ketone bodies, particularlyβ-hydroxybutyrate (B-HOB). However, no significant clinical improvement could be seen. Additional application of prednisone led to normalization of CPK serum levels.
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  • 9
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Numerous monofunctional amines have been reacted with dimethylthiophosphinic chloride to form stable derivatives. Their good gas chromatographic properties and abundant molecular ions afforded highly sensitive and specific detection by selected ion monitoring (SIM) down to the picogram level. Based upon this derivatization procedure, new methods for the determination of aniline in serum and urine by SIM were developed. The minimum detectable concentration of aniline was 500 pg ml-1. Linear response curves were obtained from 1.5 to 150 ng ml-1 using (2H5)aniline as the internal standard. Specific derivatization of a series of bifunctional amines was achieved with methyldichlorophosphine and sulfur resulting in the formation of stable phospholidine-thiones. These derivatives chromatograph well on nonpolar stationary phases, and their mass spectra exhibit abundant molecular ions. This derivatization reaction was applied to the quantification of ephedrine from urine and serum by SIM with (2H2)ephedrine as the internal standard. The detection limit of the method was 1 ng ml-1. The linearity was established in the range from 10 to 160 ng ml-1.
    Additional Material: 9 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 13 (1983), S. 469-472 
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Single phase sodiumβ-alumina solid electrolyte was found to undergo isothermal phase separation in electrochemical cells, where a large oxygen potential gradient was imposed across the electrolyte and electrodes, not reversible to sodium ions, were short circuited. The experimental conditions and evidence of decomposition are outlined and a thermodynamic explanation for the phenomenon is given. This finding may lead to new methods for making multi-layered ceramic materials involving ionic conductors.
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