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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Peanut lectin (PNL), Concanavalin A (Con A) and Ulex europaeus lectin I (Ulex) were chosen to map their binding sites in different regions of formalin fixed and paraffin embedded human central nervous system tissue and pituitary gland tissues. An extended PaP method was used for PNL and Ulex, whereas a direct peroxidase technique was employed for Con A. In astrocytes, the cytoplasm as well as the delicate processes were stained by PNL and Con A; the most conspicious binding of PNL was seen in the ependymal cells and on the surface of plexus epithelial cells; in the anterior part of the pituitary gland a selective population was PNL positive. Intracytoplasmic Con A acceptors could be demonstrated in neurons, in ependymal cells, and in plexus epithelial cells. Intracytoplasmic Con A receptors were finely granular in astrocytes, oligodendrocytes, and in some cells in the pituitary gland. Ulex binding was restricted to the vascular endothelial cells and a selective population of cells in the pituitary gland. Our results suggest that lectins may be good tools for the evaluation of their respective target cells in the central nervous system and in the pituitary gland.
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to determine the effect of routine fixation on the lectin affinity of tissue structures, we used unconjugated lectins and an indirect immunoalkaline-phosphatase method for frozen sections, and the peroxidase-anti-peroxidase method for paraffin-embedded, formalinfixed tissue sections. Fourteen hyperplastic human tonsils were used, and the results of the binding spectra of each lectin were compared. In general, the binding spectrum defected in the paraffin sections was part of the broader range of affinity obtained in the frozen sections. The lectin receptors on the cell surface were especially affected by formalin fixation. On the other hand, the paraffin sections, because of their superior morphology, showed a better localization of the cytoplasmic reaction product and discriminated the cell types more accurately. Thus, the two tissue preparations are rather complementary. In the tonsil peanut agglutinin (PNA) and periodic acid/Concanavalin A (PA/Con A) proved to be suitable tools for distinguishing exactly between the crypt and the surface epithelium. Ulex europaeus agglutinin I (UEA) is a reliable endothelial marker with a strong affinity to the crypt epithelium. In the frozen sections, PNA regularly stained follicular-centre cells on their cell surface. PNA, Helix pomatia agglutinin (HPA), soybean agglutinin (SBA) and Con A stained the histiocytic population, especially PNA which additionally stained an “asteroid” histiocyte. This cell probably corresponds to the antigen-presenting histiocyte of the T-system.
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  • 3
    ISSN: 1432-2307
    Keywords: B-CLL ; B-immunoblastic lymphoma ; Richter's syndrome ; J chain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 66-year old male with Richter's syndrome died 52 month after diagnosis of chronic lymphocytic leukaemia (CLL). The clinical course was characterized by a marked IgM hypoglobulinaemia which paralleled a chronically relapsing Herpes simplex infection. Autopsy showed a large retroperitoneal and intraabdominal tumour mass and well defined supradiaphragmatic lymphomas. Histological examination revealed a composite tumour consisting of CLL B-cell type (B-CLL) and immunoblastic malignant lymphoma of B-cell type (B-IbL). The lymphocytes bear μ-chains on their surface and to a lesser extend within their cytoplasm, the obviously defective immunoblasts produce J chains exclusively. Flow cytophotometric data seem to indicate an identical diploid stem line of the two tumours. The majority of the cells are in G0/1 phase. The CLL rarely produces mitoses, however, the IbL has a mitotic rate of 7% and a considerable proportion (33%) of cells in the phase of DNA-synthesis. This is the fourth malignant lymphoma and the second immunoblastic lymphoma to be reported that produces J chain in the absence of immunoglobulin.
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  • 4
    Electronic Resource
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    Springer
    Virchows Archiv 402 (1984), S. 297-306 
    ISSN: 1432-2307
    Keywords: Neuronal tumours ; Neuronal differentiation ; Lectin histochemistry ; Concanavalin A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Concanavalin A (Con A) acceptors have been demonstrated in large differentiated neurons in a previous paper. In order to elucidate the correlation between Con A binding in normal and neoplastic neurons and lectin binding dependence upon the differentiation grade, 26 tumours of the neuronal series were examined using formalin fixed and paraffin embedded biopsy specimen. The neoplasms included 3 gangliocytomas, 7 gangliogliomas, 1 central neuroblastoma, 11 medulloblastomas, 2 retinoblastomas, and 2 sympathicoblastomas. Well differentiated neurons in gangliocytomas and gangliogliomas expressed a high intracytoplasmic Con A acceptor density comparable to the feature in large non-neoplastic neurons. Less differentiated neurons and neuroblasts showed a weak perinuclear fine granular binding or an absolute lack of binding molecules, respectively. Our results suggest that in a variety of tumours, Concanavalin A receptor density in neurons depends upon the degree of differentiation of the cell. Well differentiated cells have a higher density than poorly differentiated neoplastic neurons.
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  • 5
    ISSN: 1432-2307
    Keywords: Lymphoepithelial carcinoma ; Peanut lectin ; Ulex europaeus lectin ; S-100 protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ten tumours of tonsillar or epipharyngeal localization showing the histological picture of “lymphoepithelial carcinoma” (Schmincke 1921) were examined immunohistochemically using Peanut lectin, Ulex europaeus lectin-I and an antiserum to S-100 protein. The findings suggest a close relationship of this type of carcinoma to the normal tonsillar crypt epithelium. The majority of tumour cells are UEA-I-positive and PNL-negative, as is the crypt epithelium, while oral mucosa is both PNL- and to a lesser extent UEA-I-reactive. Tumour areas expressing this pattern contain a large number of asteroid-shaped PNL-positive histiocytes and arachnoid-shaped histiocytes reacting with anti-S-100 protein; both cell types being probably identical and representing typical elements of the normal tonsillar crypt epithelium. Consequently, the WHO-term “nasopharyngeal carcinoma, undifferentiated type” seems to be inadequate for this type of tumour.
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  • 6
    ISSN: 1432-2307
    Keywords: Coeliac disease ; peanut lectin ; malignant histiocytosis of the intestine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We present a case of a 33 year old male with a history of early childhood diarrhoea and more recently diagnosed gluten sensitive enteropathy, who died with active disease, ulcerative proctosigmoiditis and desquamative erythrodermia associated with toxin induced shock. Autopsy revealed a tumour restricted to lymph nodes of the mesentery and the retroperitoneum. This is considered to be malignant histiocytosis of the intestine (MIH). Immunohistological examination of the diagnostic jejunal biopsy showed a pathological binding pattern for peanut lectin (PNL) within the enterocytes. This may be an expression of disturbed production or secretion of a product rich in non-reducing terminal D-galactosyl residues.
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  • 7
    ISSN: 1432-2307
    Keywords: Hodgkin's disease ; Fibronectin ; Fibroblast ; Immunoperoxidase study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The tissue distribution of fibronectin (FN) was examined using a commercial anti-FN serum, the peroxidase-anti-peroxidase (PaP) technique, and paraffin sections of 22 lymph nodes affected by Hodgkin's disease. Vascular basement membranes and reticulin fibres are selectively stained and their structural changes in this pathological condition become readily visible. In contrast to the normal lymph node and to Hodgkin's disease with lymphocytic predominance, cases of mixed cellularity disease contain individual and focally grouped cells displaying intracytoplasmic FN. In nodular sclerosis these cells with fibroblast morphology are consistently numerous in the marginal zones of the cellular nodes. Strongly reacting mastocytes probably absorbed the applied anti-serum non-immunologically. All the other cell types giving rise to the varying appearances of Hodgkin's lesions are consistently negative with respect to intracellular FN, including all forms of Hodgkin cells. We conclude that in Hodgkin's disease the immigration of FN-secreting fibroblasts is an integral part of the early sclerosing reaction, which in itself is a defence/repair mechanism closely related to scar formation.
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  • 8
    ISSN: 1432-0533
    Keywords: Peanut lectin (PNL) ; Human brain tumors ; Granular cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Peroxidase-antiperoxidase (PAP) technique was selected for visualizing the binding of peanut lectin (PNL) to the most frequent human brain tumors. The randomly selected material included neoplasms of neuroectodermal and mesenchymal origin. We employed 1–5 μm sections of routinely processed and paraffin-embedded tissues. PNL receptors were detected to a variable extent on the cell surface of astrocytoma, glioblastoma multiforme, oligodendroglioma, ependymoma, meningotheliomatous meningeoma, and plexus papilloma. In gliomas an increase in malignancy seems to be associated with a decrease in PNL binding. Except for the plexus papillomas, neuraminidase pretreatment had neither a qualitative nor a quantitative influence on the binding behavior of PNL. Intracellular PNL receptors could be detected in “granular cells” and in the perinuclear region of malignant gliomas.
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  • 9
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The β-decay of 59 ms99Rb has been studied at OSTIS. As is confirmed by RPA calculations with Nilsson model wave functions, the lowest energy levels in99Sr are consistent with rotational bands built on the [411 3/2], [413 5/2] and [422 3/2] Nilsson neutron configurations at 0, 423 and 1071 keV, respectively. All three bands have similar values of the inertial parameter ħ2/2θ indicating a nearly rigid rotor.
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