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  • 1
    ISSN: 1432-1246
    Keywords: Key words Occupational UV radiation ; Lifeguard ; Mountain guide ; Ski instructor ; Occupational disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective: Ultraviolet (UV) radiation is noted to be one of the most important risk factors for non-melanoma and melanoma skin cancers. The recent development of a spore film test chamber containing spores of Bacillus subtilis resulted in a new method of UV measurement with a spectral sensitivity profile similar to erythema-weighted data calculated from spectroradiometric measurements. Methods: The practical application of dosimeters was tested on 11 persons for 43 days, under different conditions of UV exposure in five different geographical regions. Four professional lifeguards at a public swimming pool carried dosimeters attached to their shoulders or to their caps, for 11 days. Three mountain guides attached dosimeters laterally to their heads on 27 different occasions of mountaineering activity in different mountain regions. Four ski instructors carried lateral head dosimeters during eight days of skiing in the Alps. Results: The life guards received daily UV exposures ranging from 3.6 to 9.5 minimal erythema doses (MED) (mean 5.9, SD ± 1.9). The mountain guides had personal daily UV exposures of from 4.4 to 17.1 MED (11.9 ± 3.9) and ski instructors from 2.8 to 8.8 MED (6.1 ± 1.8). Conclusions: Bacillus subtilis spore film dosimeters can be applied effectively for personal solar UV measurements of occupationally exposed persons, such as lifeguards, mountain guides and ski instructors. UV levels in these occupations exceed international limits of exposure.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 88 (2000), S. 7051-7055 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The Be-enhanced formation of CdSe quantum dots in CdSe/ZnSe heterostructures grown by migration enhanced epitaxy on (001)GaAs substrates has been investigated using photoluminescence spectroscopy, x-ray techniques (diffraction and reflectometry), and transmission electron microscopy. Coverage of the ZnSe starting surface with a fractional monolayer of beryllium selenide leads to enhanced island formation well below the CdSe thickness of 0.6 monolayer corresponding to the onset of the CdSe-rich island formation in the Be-free structures. The effect of the fractional Be coverage is demonstrated by observation of sharp lines in the photoluminescence signal from patterned mesas with dimensions down to 60 nm, which is due to the emission from individual exciton localization sites attributed to quantum dots. X-ray diffraction and reflectometry measurements on CdSe/ZnSe short-period superlattices with the submonolayer CdSe insertions confirm an enhanced roughening of the CdSe layer morphology in the case of beryllium coverage. Cross-sectional transmission electron microscopy on the SLs with BeSe fractional monolayer exhibits Cd-induced stress modulation along the CdSe sheets with a lateral scale of ∼4 nm, that can also be interpreted in favor of the BeSe-nucleated CdSe-based quantum dots. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-8221
    Keywords: macrophages ; brucellas ; antibodies ; phagocytosis ; pinocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ingestion of a heterologous brucellar luminescent antiserum by cultures of macrophages from intact and immune guinea pigs and its accumulation in the cytoplasmic granules (secondary lysosomes) and its entry into phagocytic vacuoles (phagosomes) were studied. The accumulation of antiserum in the phagosomes was judged from the appearance of specific luminescence of the phagocytosed brucellas. Accumulation of luminescent antigen-antibody complex in the cytoplasmic granules also was investigated at various time intervals after phagocytosis of brucellas treated with brucellar luminescent serum. No difference was found between macrophages from intact and immune animals as regards the character of pinocytosis of the luminescent sera. Differences between macrophages were found in the rate of passage of the antisera from the lysosomes into the phagosomes and the rate of formation of luminescent granules after phagocytosis of brucellas treated with luminescent antiserum.
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  • 4
    ISSN: 1573-8221
    Keywords: precipitation test ; light scattering spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A method of light scattering spectroscopy was used to read the precipitation test. The system investigated consisted of human serum globulin and rabbit antiserum against human globulins. By light scattering spectroscopy the precipitation test can be read in much higher dilutions of antigen and antibody than by the ordinary visual method.
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  • 5
    ISSN: 1573-8221
    Keywords: stable streptococcal L-forms ; myocardial muscle fibers ; common antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Common antigenic features of stable streptococcal L-forms and of the cytoplasmic membrane of muscle fibers of the human myocardium were demonstrated by an immunofluorescence method. The common antigen is a component of the surface membrane of the muscle cell, adjacent to the sarcolemmal sheath, and of the membranes of the transverse tubules of the macroplasmic reticulum, which pass through the sarcomeres of the muscle fiber in the zone of the L-disks. The reaction was completely prevented by exhaustion of the antiserum against antigen of L-forms by means of a human myocardial tissue homogenate or a suspension of cultures of L-forms grown on a meat or casein medium. Exhaustion with a tissue homogenate of other organs (liver) or with concentrated nutrient medium had virtually no effect on the intensity of the reaction. In the authors' view, the presence of common antigens in cultures of stable L-forms of group A streptococcus and the myocardium may be one cause of the long persistence of L-forms in the human and animal body.
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  • 6
    ISSN: 1573-8221
    Keywords: serologic reaction ; light scattering spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The possibility of recording the results of serologic reactions not yielding visible phenomena by means of light scattering spectroscopy (LSS) was studied. Influenza virus andMycoplasma pneumoniae antigens and the corresponding antisera were used as the test objects. It was shown that, in principle, LSS can be used to detect the antigen-antibody complex in serologic reactions not yielding viible phenomena.
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