Springer Online Journal Archives 1860-2000
Chemistry and Pharmacology
Abstract A quantitative analysis of malate dehydrogenase isozymes has been carried out in a hexaploid wheat Triticum aestivum variety Holdfast, a diploid rye Secale cereale variety King II, a series of seven addition lines each having the Holdfast wheat chromosome complement, and also a different homologous pair of King II rye chromosomes. In young shoots of three of these addition lines grown in a defined salts medium lacking sucrose, at least one isozyme activity was elevated. This did not occur in shoots grown in a medium containing 0.5% sucrose or in the Triticale possessing the full wheat and rye chromosomal complements grown in the absence of exogenous sucrose. On the basis of cellular localization and substrate inhibition studies, the particular isozyme activities enhanced by the rye chromosomes were indistinguishable from isozyme activities in Holdfast wheat and dissimilar to all malate dehydrogenase isozyme activities observed in King II rye. These results suggest that three different rye chromosomes produce gene products which can interact with the wheat malate dehydrogenase regulatory system.
Type of Medium: