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  • 1970-1974  (3)
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  • 1
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The acrosomes of mammalian spermatozoa contain a proteolytic enzyme which may be of importance for fertilization. The release and activity of the enzyme can be visualized directly when the spermatozoa are applied to fixed gelatin membranes. The purpose of this study was to determine the pattern of enzyme release from the spermatozoa of a variety of mammalian species. Thin, transparent membranes of pure gelatin were prepared on microscope slides and fixed in glutaraldehyde. Spermatozoa were applied to the membranes and their proteolytic behavior observed under dark-medium phase objectives at 37°C. In all the species examined (rabbit, bull, hamster, rat, guinea pig and man) extensive depolymerization of the gelatin substrate occurred around the sperm heads. With the exception of human spermatozoa, the onset of proteolysis was clearly identified with the posterior half of the acrosome. In the case of human spermatozoa, no such distinction could be made. It is suggested that the proteolytic enzyme of most mammalian spermatozoa is located in the posterior segment of the acrosome.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Direct observations of ciliary activity on the mucosal surface of mammalian oviducts have rarely been reported. Yet such studies might help to determine the relative value of ciliary action in gamete transport through the ducts. The purpose of this study was to examine the direction and rapidity of ciliary currents in the oviducts of two mammalian species, the rabbit and the pig. Fresh oviducts were slit open longitudinally and submerged in Hanks' balanced salt solution at 37°C. The movements of particulate matter, such as stained lycopodium spores and 15 μ microspherents, placed on the mucosal surface, were observed and recorded on film. In both species ciliary currents in the ampulla swept the particles toward the ampullar-isthmic junction. In the isthmus of the pig oviduct the particulate matter was transported in the reverse direction, toward the ovary. The pro-ovarian current could be demonstrated throughout the isthmus, beginning a few millimeters above the uterotubal junction. In the rabbit a similar current was detected, but it was not as extensive as in the pig. In the upper isthmus of the rabbit oviduct, particulate matter and rabbit eggs in the “mucin coat” merely rotated or “tumbled” on the surface with little movement in either direction. This is the first report of pro-ovarian ciliary currents for any mammal, and we believe these to be important for sperm transport through the isthmus. The action of cilia during egg transport is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The direction of ciliary beat has been examined in vitro in human and pig-tailed macaque (M. nemestrina) oviducts. Ducts removed under anesthesia were slit open longitudinally while submerged in Hanks solution at 37° C, and particulate matter was applied to the mucosal surface. Contrary to previous observations in pig and rabbit oviducts, transport of particles was consistently in a downward direction, toward the proximal (uterine) end, in the two species examined in this study.
    Type of Medium: Electronic Resource
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