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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A cytofluorometric apparatus with incident illumination for fluorescence excitation is described here. Cytophotometric fluorescence measurements (UV- and blue excitation) of acriflavine-acridine yellow-, coriphosphine- and pararosaniline-Schiff stained di-, tetra- and octoploid liver nuclei, leucocytes and sperms (Feulgen reaction) were found to agree with the absorbance data obtained from the same slide by means of the integrating microdensitometer. The stoichiometry of the fluorescence emission is discussed in detail. It is emphasized that not a linear, but an exponential relationship exists between the emitted fluorescence intensity and the concentration of the fluorescent substance. Cytofluorometry of Feulgen-stained nuclei has proved to be as reliable and fast as the absorbance scanning measurements at the intergrating microdensitometer.
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Beim Vergleich des Feulgen-Farbgehaltes verschiedener Zellkerne (Leberzellen, Lymphozyten, Granulozyten und Spermien) traten nach Alkoholfixierung Abweichungen der gemessenen Feulgen-Werte von den nach dem Gesetz von der DNS- Konstanz zu erwartenden DNS-Gehalt dieser Kerne auf. Verglichen mit den Feulgen-Werten der diploiden Leberzellkerne ergaben Lympho- und Granulozyten bei allen Hydrolysezeiten zu niedrige (bis zu minus 20%), haploide Spermien im postmaximalen Hydrolysebereich zu hohe Feulgen-Werte (z. T. sogar höhere Werte als die diploiden Zellkerne). Innerhalb desselben Zelltypes wurden dagegen, auch beim Vergleich der verschiedenen Ploidiestufen der Leberkerne, keine Differenzen festgestellt. Da die an Leukozyten und Spermien beobachteten Abweichungen nach Methanol-Formalin-Eisessig-Fixierung nicht mehr auftraten und auch durch UV-absorptionscytophotometrische Messungen nicht bestätigt werden konnten, muß man annehmen, daß es sich um Proportionalitätsfehler handelt, die auf Hydrolyseunterschieden beruhen. Für die quantitative Feulgen-Cytophotometrie scheint daher die Methanol-Formalin-Eisessig-Fixierung besser geeignet zu sein als die Alkoholfixierung, bei deren Verwendung es leicht zu Proportionalitätsfehlern während der Feulgen-Hydrolyse kommen kann.
    Notes: Summary Comparing the Feulgen dye-content of different nuclei (liver cells, lymphocytes, granulocytes and sperms) after alcohol-fixation deviations were found between the measured Feulgen values and the DNA-content to be expected from the DNA-constancy law. The Feulgen values of lymphocytes and granulocytes proved to be lower (up to minus 20%) than those of diploid liver nuclei at all hydrolysis times, while in the postmaximal range of hydrolysis the values of the haploid sperms were too high (even higher than those of the diploid nuclei). Such differences did not appear when nuclei of the same cell type in different DNA- ploidy classes (liver nuclei) were compared. Those deviations of leucocytes and sperms were no longer found after fixation in methanol-formalin-glacial acetic acid and, in addition, could not be confirmed by UV-absorption measurements. For that reason we suppose them to be due to proportionality errors caused by variations in the hydrolytic behaviour of the different nucleoproteins. Thus fixation in methanol-formalin-glacial acetic acid seems to be more suitable for quantitative Feulgen measurements than alcohol-fixation, which may easily give rise to proportionality errors during Feulgen hydrolysis. Moreover, to avoid any false interpretation of Feulgen data we should suggest controll measurements using another independent method (f. e. UV-absorption), or — if this is impossible — to check the Feulgen values after different fixations and variant hydrolysis times (premaximal, maximal, postmaximal).
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  • 3
    ISSN: 1432-1076
    Keywords: Hepatitis epidemica ; Serumbilirubin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. An Hand eines Krankengutes der Jahre 1963–1965 konnte die früher beschriebene Korrelation zwischen Serumbilirubin und Lebensalter bei Kindern mit Hepatitis epidemica erneut nachgewiesen werden. 2. In gleicher Weise besteht eine Korrelation zwischen Serumbilirubin und Körperoberfläche. Jedoch vermittelt diese keine deutlich bessere oder zusätzliche Information im Vergleich zum Lebensalter. 3. Beide Korrelationen werden unter epidemiologisch definierten Bedingungen besonders deutlich. Inhomogenitäten des untersuchten Krankengutes beeinträchtigen den Nachweis. 4. Korrelationen zwischen SGPT und Lebensalter sowie Krankheitstagen konnten in dieser Untersuchung nicht nachgewiesen werden. Alle Angaben beziehen sich auf die ersten 12 Krankheitstage bei einem klinischen Krankengut.
    Notes: Summary 1. The correlation between the level of bilirubin in the serum and the age of children with Hepatitis epidemica as described in a previous communication was demonstrated once more among other groups of children. 2. There exists a correlation between the level of bilirubin in the serum and bodysurface in children as well. But application of this parameter did not improve the results as compared with age. 3. The selection of groups of children by epidemiological markers improved the correlations. It is assumed that heterogeneity of groups caused negative results of correlation and regression analysis. 4. No association could be demonstrated between SGP transaminases and age or days of illness. All statements refer to the period of 12 days after onset of the disease.
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  • 4
    ISSN: 1433-8491
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 99 (1968), S. 389-389 
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 6
    ISSN: 1591-9528
    Keywords: Skin wound ; Total body X-irradiation ; Plasma corticosterone ; Hautwunde ; Röntgenganzkörperbestrahlung ; Plasmacorticosteron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Männliche Albinomäuse wurden gruppenweise Einzelbelastungen wie Äthernarkose, Hautwunde und Röntgenganzkörperbestrahlung von 250 R ausgesetzt. Dabei zeigte sich ein grundsätzlich gleichartiges Verhalten der Plasma-Corticosteronkonzentration mit kurzfristigem initialem Anstieg und nachfolgendem Abfall unter den entsprechenden Wert des Tagesprofils unbehandelter Kontrolltiere. 2. Das kombinierte Trauma bestand aus einer Hautwunde mit nachfolgender Röntgenganzkörperbestrahlung von 250 R. Der zeitliche Abstand zwischen beiden Belastungen betrug 24 und 96 Std. Dabei fand sich nach einem 24stündigen Intervall ein wesentlich stärkerer und länger andauernder Anstieg der Steroidkonzentration. 3. Die bei kombinierten Traumen mit 24stündigem Intervall vonFridrich (1963) undFlemming u.Flemming (1964) beobachtete Senkung der Mortalität ließe sich somit durch die verstärkte Corticoidsekretion der Nebennierenrinde erklären.
    Notes: Summary 1. Male albino mice were subjected to single traumata like aether anaesthesia, skin wound and total body X-irradiation of 250 R. In all animals the plasma corticosterone level showed a brief initial elevation with a following decrease under the corresponding value of resting animals. 2. The combined trauma consisted of a skin wound with following total body X-irradiation of 250 R. The interval between the two traumata was 24 and 96 h. The plasma corticosterone level showed a higher and longer elevation after the 24 h interval. 3. The decrease in mortality reported byFridrich (1963) andFlemming andFlemming (1964) in combined injuries with an interval of 24 h could be explained by the elevated level in plasma corticosterone.
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  • 7
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
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  • 8
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of organic chemistry 31 (1966), S. 2402-2403 
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The use of electrophotography for the detection of non-coloured, chromatographed compounds showing absorption maxima in the longwavelength ultraviolet range is described.
    Notes: Zusammenfassung Die Anwendung der Elektrophotographie zur Detektion farbloser, chromatographisch entwickelter Verbindungen mit Absorptionsmaxima im langwelligen UV-Bereich wird beschrieben.
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