Springer Online Journal Archives 1860-2000
Summary 1. Methane fermentation of Ca-acetate is about as sensitive to phenylhydrazine, hydroxylamine and azide as it is to sulphite (inhibition by 10−2 m 70, 83, 94 and 95 % resp.; by 10−3 m 0, 39, 49 and 31 % resp.). 2. The sensitivity to dimedone is much less, 10−2 m inhibiting 17% only. 3. The process is much more sensitive to cyanide, 5.10−4 m giving already ±90% inhibition. 4. The sensitivity to sulphide is much less, as 5.10−2 m is necessary for complete inhibition, whereas 10−2 m does not inhibit at all. 5. Cultures of methane bacteria on Ca-acetate can be accustomed to 0.15 % formaldehyde which is fermented to CH4 and CO2 in presumably equimolecular proportions. 6. The results are discussed in the light of data on the action of these toxic substances in biological systems in general, with special reference to anaerobic bacteria.
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