Life and Medical Sciences
Cell & Developmental Biology
Wiley InterScience Backfile Collection 1832-2000
Rats in different stages of maturity were castrated in pairs-bilaterally and unilaterally-with respect to the effects upon the epipidymis. The epididymis was also studied during its developmental stages. A special study was made of the mitochondria and Golgi materials; and a comprehensive review of the literature is included.Bilateral castration was followed by a marked regression of functional activity on the part of the epithelial cells of the epididymis. Unilateral castration produced no similar change. The mitochondria-Golgi complex in the epithelial cells of the epididymis was reduced decidedly following bilateral castration; while unilateral castration had no visible effect upon these materials. The mitochondria-Golgi complex increased and decreased in amounts in keeping with the state of secretory activity of the epithelial cells.Golgi nets were found within the lumina of the tubules. Granules within the cells and the tubules were colored with osmic acid, Altmann's anilin acid-fuchsin, iron hematoxylin, or acid fuchsin-orange G. Osmic acid impregnation was removed from granules and nets and Altmann's anilin acid-fuchsin stain put in its place.It is believed that mitochondria and Golgi materials are very similar and furthermore that they are secretory products of the cells. It is also believed that the state of development of the mitochondria-Golgi complex is an indication of the functional state of the epithelial cells of the epididymis.
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