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  • Blackwell Publishing Ltd  (2)
  • 1
    ISSN: 1745-4514
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Proteases recovered from Northern pink shrimp (NPS) and Southern rough shrimp (SS) processing discards (heads, shells, tails) were characterized. Shrimp processing discards were extracted with water following homogenization and centrifugation in order to obtain the crude extract which was subsequently fractionated with solid ammonium sulfate. Two fractions sedimenting with 30–50% (designated as NPS-I and SS-I), and 50–70% (designated as NPS-II and SS-II) ammonium sulfate were collected following centrifugation, respectively. Endoprotease activity of the crude extract was 0.02 U/mg for hemoglobin (Hb, pH 3.0), 0.16 U/mg for azocasein (Ac, pH 6.0) and 0.12 U/mg for benzoyl-Arg-β-naphthylamide (BANA, pH 7.0). The exoprotease activity was 0.11–0.17 U/mg for Arg-β-naphthylamide (ArgNA, pH 7.0), 0.06–0.11 U/mg for Lys-β-naphthylamide (LysNA, pH 7.0) and 0.08–0.09 U/mg for Leu-β-naphthylamide (LeuNA, pH 7.0). Endoprotease activity increased 5–7.4 fold for NPS-I and SS-I, and 1.9–2.7 fold for NPS-II and SS-II against crude extract. Meanwhile, exoprotease activity increased 3.6–4.8 fold for NPS-I and SS-I, and 5.6–7.2 fold for NPS-II and SS-II. Meat treated with NPS-I and SS-I was tenderized more extensively than that treated with NPS-II and SS-II. The results of this study suggest that proteases recovered from shrimp processing discards may potentially be used as processing aids in formulated foods.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 70 (2005), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The physicochemical properties of acid-soluble collagen (ASC) from refiner discharge and the partially purified collagen (PPC) from both the refiner discharge and the fish skin were evaluated. Yield of collagen from refiner discharge was 34% higher in PPC than ASC. Mercury, lead, cadmium, and chromium contents of PPC from refiner discharge were not detected. There was no difference in the pattern of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) between ASC and PPC from refiner discharge. PPC from refiner discharge showed better functional properties than that from skin and was similar to ASC: whiteness, solubility, emulsifying activity, and cooking stability. Therefore, PPC from refiner discharge could be used as a new resource.
    Type of Medium: Electronic Resource
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