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  • 1
    Keywords: Life sciences ; Cytology ; Cell Culture ; Life sciences ; Cell Biology ; Cell Culture ; Springer eBooks
    Description / Table of Contents: 1. Detection of Mycoplasma Contaminations -- 2. Eradication of Mycoplasma Contaminations -- 3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination -- 4. Classical and Molecular Cytogenetic Analysis -- 5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle -- 6. The Development of T Lymphocytes in Fetal Thymus Organ Culture -- 7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors -- 8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells -- 9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media -- 10. Isolation and Characterization of Mouse Side Population Cells -- 11. Stem Cell Identification by DyeCycle Violet Side Population Analysis -- 12. Isolation and Characterization of Cancer Stem Cells In Vitro -- 13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets -- 14. Generation and Characterization of Murine Alternatively Activated Macrophages -- 15. Human Long Term Culture Initiating Cell (LTC-IC) Assay -- 16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells -- 17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells -- 18. Studying Leukocyte Recruitment Under Flow Conditions -- 19. Generation and Establishment of Murine Adherent Cell Lines -- 20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture -- 21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone -- 22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture -- 23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells -- 24. Human Prostate Epithelial Cell Cultures -- 25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue -- 26. Isolation and Characterization of Human Hair Follicle Epithelial Cells -- 27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells -- 28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells -- 29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells -- 30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System -- 31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells -- 32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates -- 33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
    Abstract: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems.℗ Written in the highly successful Methods in Molecular Biologý„Ø, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ℗ Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success
    Pages: XIV, 550 p. 104 illus., 44 illus. in color. : digital.
    Edition: 4th ed.
    ISBN: 9781627031288
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  • 2
    Call number: QH506:60/290a
    Keywords: Cell Culture Techniques / methods ; Culture Techniques / methods
    Description / Table of Contents: Culture of primary adherent cells and a continuously growing non-adherent cell line / Cheryl D. Helgason -- Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Authentication of scientific human cell lines / Wilhelm G. Dirks and Hans G. Drexler -- Cytogenetic analysis of cell lines / Roderick A.F. MacLeod and Hans G. Drexler -- Human and mouse hematopoeitic colony forming cell assays / Cindy L. Miller and Becky Lai -- Isolation and culture of murine macrophages / John Q. Davies and Siamon Gordon -- Isolation and culture of human macrophages / John Q. Davies and Siamon Gordon -- The development of T lymphocytes in mouse fetal thymus organ culture / Tomoo Ueno ... [et al.] -- In vitro generation of lymphocytes from embryonic stem cells / Renée F. de Pooter, Sarah K. Cho and Juan Carlos Zúniga-Pflücker -- Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- Generation of murine stromal cell lines / Robert A.J. Oostendorp, Kirsty Harvey and Elaine E. Dzierzak -- Culture of human and mouse mesenchymal cells / Emer Clarke -- Isolation, purification, and cultivation of rat and human keratinocytes / Frizell L. Vaughan and Ludmila I. Bernstam -- Isolation and culture of primary human hepatocytes / Edward L. LeCluyse ... [et al.] -- Primary kidney proximal tubule cells / Mary Taub -- Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity / John Stingl, Joanne T. Emerman, and Connie J. Eaves -- Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue / Sharon A. Louis and Brent A. Reynolds -- Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Gabi Shefer and Zipora Yablonka-Reuveni -- Adult ventricular cardiomyocytes / Klaus-Dieter Schlüter and Daniela Schreiber -- Isolation and culture of primary endothelial cells / Bruno Larrivée and Aly Karsan -- Studying leukocyte rolling and adhesion in vitro under flow conditions / Susan L. Cuvelier and Kamala D. Patel -- Isolation and characterization of side population cells / Margaret A. Goodell, Shannon McKinney-Freeman and Fernando D. Camargo -- Scalable production of embryonic stem cell-derived cells / Stephen M. Dang and Peter W. Zandstra
    Notes: 2nd ed. edited by Jeffrey W. Pollard and John M. Walker.
    Pages: xii, 371 p. : ill. + 1 CD-ROM
    Edition: 3rd ed.
    ISBN: 1588292843
    Signatur Availability
    QH506:60/290a available
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