Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Chemistry and Pharmacology
The hexameric 12S central subunit of transcarboxylase has been crystallized in both free and substrate-bound forms. The apo crystals belong to the cubic space group P4232, with unit-cell parameters a = b = c = 188.5 Å, and diffract to 3.5 Å resolution. Crystals of two substrate-bound complexes, 12S with methylmalonyl CoA and 12S with malonyl CoA, are isomorphous and belong to space group C2, with unit-cell parameters a = 115.5, b = 201.4, c = 146.9 Å, β = 102.7°. These crystals diffract to 1.9 Å resolution with synchrotron radiation. Two useful heavy-atom phasing derivatives of methylmalonyl CoA-bound crystals have been obtained by co-crystallization or crystal soaking.
Type of Medium: