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  • 1
    ISSN: 1432-1076
    Keywords: Osteogenesis imperfecta ; Collagen types ; Bone ; In vitro study ; Immunofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Samples of bone from a patient with osteogenesis imperfecta were found to synthesize and contain type III collagen as well as type I collagen. Normal bone contains only type I collagen except in the lining cells of the bone marrow cavities. In the patient's tissue, type III collagen was localized in nonfibrillar structures in discrete areas of the bone. These and previous studies indicate that certain types of osteogenesis imperfecta may be caused by a failure of normal bone maturation and the sites in which the type III collagen is found appear to be defects in the bone.
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  • 2
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 21 Lebercirrhosen unterschiedlicher Ätiologie wurden mit Hilfe der indirekten Immunfluorescenztechnik mit typ-spezifischen Kollagenantikörpern auf Vorkommen und Verteilung einzelner Kollagentypen untersucht. In cirrhotischen Bindegewebssepten und Portalfeldern fand sich in allen Cirrhosen Kollagen Typ III stark vermehrt. Dagegen ließen sich mit typ-spezifischen Antikörpern gegen Kollagen Typ I und II keine anderen Kollagentypen nachweisen. Damit werden biochemische Untersuchungen bestätigt, die in Lebercirrhosen neben Kollagen Typ III einen neuen, bisher nicht beschriebenen Kollagentyp zeigen, der elektronenoptisch als Kollagen Typ I erscheint, sich jedoch biochemisch und immunologisch anders verhält. Eine Korrelation zwischen Ätiologie der Cirrhosen und Auftreten oder Verteilungsmuster bestimmter Kollagentypen konnte bisher nicht gefunden werden.
    Notes: Summary Using indirect immunofluorescence technique, 21 cases of hepatic cirrhosis of differing etiology were studied with type-specific antibodies to collagen type I, II, and III. In all cases the fibrous septa and portal tracts showed an increase in type III collagen. No fluorescence could be observed with antibodies to collagen type I and II. Thus, biochemical studies are supported which show, in addition to type III collagen, a new, as yet undescribed type of collagen in liver cirrhosis that is similar to type I collagen electronmicroscopically, but differs from type I collagen biochemically and immunologically. No correlation between the etiology of cirrhosis and the pattern of different collagen types could be found. The origin of different collagen types in liver cirrhosis is briefly discussed.
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  • 3
    ISSN: 1432-0509
    Keywords: Radiation nephritis ; Kidney, nephrogram-Kidney, CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We present two cases showing bilateral segmentai delayed nephrograms secondary to radiation injury which occurred during radiation therapy for retroperitoneal adenopathy. The abnormal areas in the affected kidneys were not devoid of a nephrogram, but rather showed a persistent corticomedullary separation indicative of slowed perfusion. Linear margins not conforming to the normal renal lobular configuration were a confirmatory feature. Radiation injury should be included in the differential of both the asymmetric prolonged nephrogram and the “segmental” nephrogram.
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  • 4
    ISSN: 1432-069X
    Keywords: Collagen autoantibodies ; Epidermolysis bullosa ; Bullous diseases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to determine whether autoantibodies are present in sera from normal individuals and/or patients with selected bullous disorders, a highly sensitive solid-phase radioimmune assay was established using purified native collagen types I–VI, laminin, and fibronectin as substrates. Sixty-four sera were utilized, representing 12 normal controls as well as 4 patients with extensive thermal burns, 18 with autoimmune bullous diseases (11 bullous pemphigoid, 5 pemphigus vulgaris, and 2 epidermolysis bullosa acquisita), and 30 with non-autoimmune mechanobullous diseases [epidermolysis bullosa (EB): 20 simplex, 4 junctional, and 6 dystrophic]. In general, autoantibodies to types I, II, and VI collagen and fibronectin were undetectable in any of the patient or control groups. In contrast, autoantibodies to types III and V collagen were noted in 87.5% (28/32) and 90.6% (29/32) of EB sera, respectively, while being only rarely noted in sera from other patient groups. Similarly, autoantibodies to type IV collagen and laminin were detected in 50% (16/32) and 40.6% (13/32) of EB sera, especially from patients with simplex and dystrophic forms of the disease. These data suggest that selected interstitial and basement membrane-associated collagens and laminin may become autoimmunogenic in all three forms of inherited EB in contrast to their relative lack of immunogenicity in at least some of the other intraepidermal and subepidermal blistering disorders. The role, if any, of these autoantibodies in the induction or perpetuation of blistering in EB awaits further studies.
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  • 5
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Female MRL/lpr mice were treated with I.P. doses of methotrexate (MTX) and 10-deazaaminopterin (DAAM) in the range of 1 to 100 mg/kg body weight/week, in two equally divided doses. Treatment began at 7 weeks of age and continued to 30 weeks of age. Joint histopathology scores were tightly correlated with skin lesion-proteinuria scores at 30 weeks of age. MTX at levels of 5, 25, and 100 mg/kg body weight/week and DAAM at a level of 25 mg/kg body weight/week significantly reduced skin lesion-proteinuria scores below controls in a dose dependent manner. Animals receiving MTX at 25 mg/kg body weight/week had a significantly longer median life span and animals receiving MTX at 100 mg/kg body weight/week had a greater than 15% suppression of growth when compared with cottrols. Longevity and skin lesion-proteinuria scores appeared to be good indicators of drug efficacy while growth suppression appeared to be a good indicator of drug toxicity.
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  • 6
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0340-1855
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
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  • 8
    ISSN: 1432-1335
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Several benign and malignant tumors of bone and cartilage were examined by means of type-specific collagen antibodies in connection with indirect immunofluorescence technique in order to determine wether there is a positive correlation between cell morphology and gene expression as refered to the synthesis of tissue- or cell-specific collagen. In general benign bone and cartilage tumors show the collagen type corresponding to the original maternal tissue. In malignant osteogenic tumors a strong positive correlation was found between morphologic differentiation of osteosarcoma cells and tissue specific collagen synthesis (collagen type I). In general this correlation was not found in chondrosarcomas. Unrelated to the grade of differentiation and the type of malignant tumor, collagen type III could be demonstrated in all tumors investigated, occurring rather from vascular stroma than from the tumor cell itself.
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  • 9
    ISSN: 1432-1440
    Keywords: Kollagentypen ; Plättchenaggregation ; Collagen types ; platelet aggregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Collagen types I, II and III from man and calf are distinctly different with respect to their effect on the induction of the aggregation of human platelets. Collagen type III was found to be a particularly potent inducer of platelet aggregation. The pathogenetic implications of these observations are briefly discussed.
    Notes: Zusammenfassung Die Kollagentypen I, II and III von Mensch und Kalb weisen in löslicher und strukturierter Form deutliche Unterschiede in ihrer Wirkung auf die Aggregation menschlicher Thrombocyten auf. Eine besonders stark aggregierende Wirkung wurde für das Typ III Kollagen gefunden. Die pathogenetische Bedeutung dieses Befundes wird kurz diskutiert.
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  • 10
    ISSN: 1432-1440
    Keywords: Kollagen ; Immunfluorescenz ; Thrombus ; Blutplättchen ; Collagen ; immunofluorescence ; thrombus ; platelets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Sections of arterial walls and of thrombi and smears of leukocytes previously incubated in vitro with collagen type III were examined by immunohistochemical technique for the presence of collagen types I, II and III. In arterial walls collagen type III was detected immediately underlaying the endothelial cell layer and in the tissue between tunica elastica interna and adventitia. Collagen type I was not shown in the subendothelial layer. Fresh thrombi contained occasionally collagen, but only of type III. This was associated with leukocytes. Leukocytes were capable in vitro to associate and/or phagocytose collagen type III and this could be visualized immunohistochemically. The data show that collagen type III in vivo may play a crucial role in the initiation of thrombus formation.
    Notes: Zusammenfassung Schnitte von arteriellen Gefäßwänden und von Thromben sowie Ausstriche von Leukocyten, die in vitro mit Kollagen Typ III inkubiert worden waren, wurden mit Hilfe der indirekten Immunfluorescenz auf die Verteilung und Anwesenheit von Kollagen Typ I, II und III untersucht. In Arterienwänden wurde Kollagen Typ III direkt subendothelial gefunden, sowie zwischen Tunica elastica interna und Adventitia. Kollagen Typ I war in der subendothelialen, der Tunica elastica interna direkt aufliegenden Schicht nicht nachweisbar. Frische Thromben enthielten gelegentlich Kollagen, jedoch nur Typ III. Dieses war mit Leukocyten assoziiert. Auch in vitro waren Leukocyten in der Lage, Kollagen Typ III zu assoziieren und/oder phagocytieren. Solches Kollagen war immunohistochemisch nachweisbar. Die Ergebnisse zeigen, daß Kollagen Typ III vermutlich eine entscheidende Rolle bei der arteriellen Thrombusbildung spielt.
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