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  • 1
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    Elsevier Science, San Diego, C
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  • 2
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Epidermal growth factor (EGF) is a peptide which stimulates cell mitotic activity and differentiation, has a cytoprotective effect on the gastroduodenal mucosa, and inhibits gastric acid secretion. The immunohistochemical localization of EGF in the Brunner's glands and the submandibular glands is well documented. The localization of EGF in other tissues is still unclarified. In the present study, the immunohistochemical localization of EGF in tissues from rat, man and a 20 week human fetus were investigated. In man and rat, immunoreaction was found in the submandibular glands, the serous glands of the nasal cavity, Brunner's glands of the duodenum, the Paneth cells of the small intestine, and the tubular cells of the kidney. In the fetus EGF was found in the kidney and in the intestinal Paneth cells. Antisera raised against rat submandibular EGF did not recognize EGF in human tissues, whereas antisera against human urinary EGF worked in rat as well as man. EGF was found only in cells with an exocrine function.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1433-0393
    Keywords: Key words Pregnancy • Delivery • Adult congenital ; heart disease ; Schlüsselwörter Schwangerschaft • Angeborene Herzfehler • Erwachsene • Entbindung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Prognose angeborener Herzfehler hat sich in den letzten Jahrzehnten dramatisch verbessert. Gleichzeitig hat auch die Zahl von Frauen, die mit einem angeborenen Herzfehler schwanger werden, so stark zugenommen, daß diese inzwischen 30–50 % aller kardialen Erkrankungen bei Schwangeren ausmachen. Die Mehrzahl der Patientinnen mit angeborenen Herzfehlern hat eine oder mehrere Korrekturoperationen überstanden. Angeborene Herzfehler in ihrer nativen Form, kommen wesentlicher seltener vor. Während der Schwangerschaft kommt es zu Umstellungsreaktionen, die – in Abhängigkeit vom zugrundeliegenden Herzfehler – die Hämodynamik in unterschiedlicher Weise beeinflussen. Die veränderte Hämodynamik muß für jeden Herzfehler gesondert abgeschätzt werden und erfordert eine individuelle Betreuung der Patientinnen. Probleme betreffen die Auswirkung der Schwangerschaft auf die Grunderkrankung, sowie die Auswirkung der Grunderkrankung auf den Schwangerschaftsverlauf und den Fetus. Die Schwangerenberatung beinhaltet Informationen über genetische Risiken, potentiell teratogene Substanzen, Schwangerschaftsverlauf, Entbindung und postpartale Nachsorge. Heutzutage können die meisten Patientinnen mit angeborenen Herzfehlern eine Schwangerschaft gut bewältigen, wenn eine enge Kooperation zwischen Kardiologen und Gynäkologen gewährleistet ist.
    Notes: Summary Because of advances in medical treatment and surgical management the number of adolescent females with congenital heart disease increased significantly during the last decade. On the other hand, the incidence of rheumatic heart disease, which accounted for the largest number of pregnant women with heart disease in the past, has declined over the years. Pregnancy is associated with a lot of physiological adjustments, such as changes in blood volume, stroke volume and cardiac output and may result in deleterious clinical effects in patients with congenital heart disease. Although a good outcome for these woman can often be expected, congenital heart disease still represents a high-risk pregnancy for both mother and infant. The actual risk depends on the type of malformation and functional impairment of the mother. To accompany these women safely through pregnency, good cooperation between pediatric and adult cardiologists, obstetrician and other physicians is necessary.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2307
    Keywords: Mouse teratoma ; Centrifugal elutriation ; Melanotic neuroepithelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Dissociation and elutriation procedures were applied to the OTT-6050 mouse teratoma line carried in subcutaneous implants in 129/J mice in order to enrich the differentiating neuroepithelial cells. Subsequent renal subcapsular implantation of one of the resultant cell fractions (IB-9) in syngeneic mice led to the constant production of macroscopically pigmented tumors which, in addition to undifferentiated stem cells, contained primitive neuroepithelial populations composed of medullary epithelium, neuroblasts, and numerous ependymoblastic rosettes. Melanin pigment, confirmed by the presence of melanosomes and premelanosomes, was found in medullary epithelium and other primitive neural cells. The tumors preserved their characteristics through 65 sequential transplants over a period of 5 1/3 years. The pigment was maintained in vitro for up to 3 months in an organ culture system. Subcutaneous or intracerebral transplantation of the renal tumors of the IB-9 fraction accentuated the capacity of these primitive cells towards further neuroepithelial differentiation into mature synapse-forming neurons, and was associated with a decrease in primitive neuroepithelium and an absence or a marked decrease of melanin. Return of the tumor to the kidney resulted in the reappearance of melanin after one to three passages, again associated with the presence of primitive neuroepithelium. The recognition of melanin pigment in the OTT-6050 mouse teratoma transplants could be a useful marker for the successful selection of primitive neuroepithelial cell populations in this experimental tumor system. These populations may help to study the relationship between melanin production and certain types of primitive neuroectodermal tumors in man.
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  • 5
    ISSN: 1432-2218
    Keywords: Key words: Pneumoperitoneum, physiopathology — Laparoscopy, side effects — Locoregional therapy — Technology, medical
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Multimodal therapy is used increasingly in advanced gastrointestinal tumors. Potential benefits of using an intraoperative adjuvant therapy during laparoscopy for cancer have been documented in animal studies. The aim of this study was to develop a device that could deliver such an intraoperative drug therapy. Methods: We developed a micropump suitable for minimally invasive surgery procedures that allowed microdroplets of therapeutic substance to be distributed into the pneumoperitoneum (CO2), creating a ``therapeutic pneumoperitoneum.'' A closed-loop control system regulates drug delivery according to the gas flow. In vitro, the micropump is able to aerosolize various aqueous and ethanol solutions, including cytostatic and bacteriostatic drugs and adhesion-modulating agents. The size of the microdroplets has been optimized to prevent visual artifacts. Results: The micropump was tested in an animal model (pig). The system was inserted into a 5-mm trocar. After insufflation of a 12-mm CO2 pneumoperitoneum, laparoscopic sigmoid colon resections could be performed with no special difficulties. No fog developed, and no system-related complication was observed. At autopsy, the active principle was distributed to all exposed peritoneal surfaces. Conclusions: As opposed to conventional peritoneal washing, therapeutic pneumoperitoneum reaches the entire peritoneal surface, allowing an optimal drug distribution. Drug diffusion into the tissues is enhanced by the intraperitoneal pressure. Precise determination of the instantaneous and total drug quantity is possible. Therefore, this drug delivery system has several advantages over conventional irrigation. Its potential domains of application are locoregional cancer therapy, prevention of port-site recurrences, immunomodulation, analgesia, peritonitis, and prevention of postoperative adhesions.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0533
    Keywords: Astrocytoma ; Cell membrane ; Microvilli ; Coated vesicles ; Surface morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Surface membrane microprojections and coated vesicles of a well differentiated (Kernohan-Sayre grade I) cerebral fibrillary astrocytoma were compared with those of anaplastic astrocytic (grade IV, glioblastoma) cerebral tumors. Both phenomena increase with increased malignancy. This provides further evidence for membrane alterations with malignant change in human astrocytic cells. It is the first comparison of fibrillary astrocytoma surface phenomena with those of more malignant astrocytic tumors of the cerebrum.
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  • 7
    ISSN: 1432-0533
    Keywords: Glioblastomas ; Organ culture method ; Autoradiography ; Kinetics ; Growth fraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five human glioblastomas maintained in an organ culture system were studied by autoradiography to determine, after 8 days in vitro, the growth fraction (GF) of the explants, their total cell cycle time (T C) and cell cycle phase durations (T S,T G1,T G2 andT M), and their potential doubling time (T pot) after pulse-labeling with [3H] TdR for 1 h. These parameters were derived from computer analysis of fraction of labeled mitoses (FLM) curves. The results fell into two groups. In two tumors, the cultures had a GF of 0.25 and 0.23. From the FLM curves were derived aT C of 89 and 83 h, aT S of 16.5 and 9.5 h, and aT G1 of 60 and 61 h.T M was estimated at 0.9 and 0.6 h, andT G2 12h. TheT pot was 12 days. These values approximate those reported for glioblastomas and other human malignancies in vivo. The explants of three other glioblastomas gave different FLM curves: the derivedT S were increased to 36 and 55 h, estimatedT M ranged from 2.4 to 4.5 h, andT G2 ranged from 11 to 20 h.T C andT G1 could not be estimated. In two tumors the GF was reduced to 0.12 and 0.11, with aT pot of respectively 52 and 39 days. These values are comparable to those reported for astrocytomas of intermediate malignancy. In the third tumor, the GF was only 0.014. The reduction in GF and the lengthening of cell cycle components in this group of explants are similar to the kinetic changes reported in some in vivo tumors and three-dimensional in vitro systems that have reached a plateau stage of growth. They are probably related to the greater opportunities for cell-to-cell contacts and the resulting increased differentiation favored by the organ culture technique.
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  • 8
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The glycoprotein of Reissner's fibre is able to bind norepinephrine, epinephrine and serotonin. Bound amines can be demonstrated at the RF, even after washing with artificial cerebrospinal fluid for some, hours. It is discussed whether it is a function of the RF to remove substances, for instance biogenic amines, from the cerebrospinal fluid.
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  • 9
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report here the complete nucleotide sequence of the E. coli triose phosphate isomerase gene. The gene encodes a polypeptide of 255 amino acids which is approximately 46% homologous to eukaryotic triose phosphate isomerases, and approximately 38% homologous to the enzyme from a thermophilic bacterium, Bacillus stearothermophilus. The nucleotide sequence is 55% homologous to that of the corresponding gene in the yeast Saccharomyces cerevisiae. To our knowledge, this is the first report of the sequence of a gene coding a glycolytic enzyme from a prokaryotic organism.
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  • 10
    ISSN: 1617-4623
    Keywords: Key wordsListeria monocytogenes inlGHE locus ; inlC2DE locus ; internalin multigene family ; leucine-rich repeat (LRR) proteins ; Listeria virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this work we identified and characterized a gene cluster containing three internalin genes of Listeria monocytogenes EGD. These genes, termed inlG, inlH and inlE, encode proteins of 490, 548 and 499 amino acids, respectively, which belong to the family of large, cell wall-bound internalins. The inlGHE gene cluster is flanked by two listerial house-keeping genes encoding proteins homologous to the 6-phospho-β-glucosidase and the succinyl-diaminopimelate desuccinylase of E. coli. A similar internalin gene cluster, inlC2DE, localised to the same position on the L. monocytogenes EGD chromosome was recently described in a different isolate (Dramsi S, Dehoux P, Lebrun M, Goossens PL, Cossart P (1997) Infect Immun 65: 1615–1625). Sequence comparison of the two inl gene clusters indicates that inlG is a new internalin gene, while inlH was generated by a site-specific recombination, leading to an in-frame deletion which removed the 3′-terminal end of inlC2 and the 5′-terminal part of inlD. The third gene of the inlGHE cluster, inlE, is almost identical to the previously reported inlE gene. Our data show that the inlGHE gene cluster is probably transcribed from a major PrfA- independent promoter located upstream of inlG. PCR analysis revealed the presence of the newly identified inl genes inlG and inlH in most L. monocytogenes isolates tested. A mutant which has lost inlG, inlH and inlE by an in-frame deletion exhibited, after oral infection of mice, a significant loss in virulence and shows drastically reduced numbers of viable bacteria in both liver and spleen when compared to the wild-type strain.
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