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  • 1
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA ; Cybrids ; B. napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An important mitochondrial (mt) DNA polymorphism was detected by SalI restriction enzyme analysis in five Brassica napus cybrids plants which combine B. napus chloroplasts and a cytoplasmic male sterility (cms) trait from Raphanus sativus. Novel restriction fragments observed in these cybrids were analysed. One of them was found to be constituted by fragments of both parent mt genomes. Sites involved in rut recombination in cybrids were compared by molecular hybridization to sites supposedly implicated in intragenomic mt recombination in B. oleracea The results indicate that mt recombination events arising through protoplast fusion involve several different rut DNA regions. Some of these regions appeared homologous to regions presumably involved in intragenomic mt recombination in B. oleracea.
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  • 2
    ISSN: 1432-0983
    Keywords: Somaclonal variation ; Mitochondrial DNA ; Protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One fertile and two male-sterile diploid plants were regenerated from the same callus after two cycles of protoplast culture from fertile Nicotiana sylvestris. Genetic studies indicated that the male-sterile trait was under cytoplasmic control. Progenies of each regenerated plant possessed different mitochondrial (mt) DNA restriction patterns. Both cms protoclone types were characterized by specific mtDNA deletions. In addition, a 40 kD mitochondrially encoded polypeptide is lacking in the cms plants.
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  • 3
    ISSN: 1432-0983
    Keywords: mtDNA ; Recombination ; CMS ; N. sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two cytoplasmic male-sterile plants (CMSI and CMSII) were obtained by protoplast culture in Nicotiana sylvestris. Both plants showed large deletions (up to 50 kb) in their mitochondrial DNA. Restriction maps of the reorganized regions suggested that the deletions occurred via two homologous recombination events (rec. 1 and rec. 2) in the parental mitochondrial genome. With the exception of nad5, no mitochondrial DNA polymorphism could be detected between parental and CMS lines using different heterologous genes probes. A sequence homologous to the Oenothera nad5 mitochondrial gene was located close to the CMSI-specific rec. 2 region. Moreover, a cDNA probe corresponding to total mitochondrial RNA from the parent line was found to hybridize to mitochondrial DNA fragments involved in the rec. 1 event common to both CMS lines, suggesting that rec. 1 lies in a transcribed region. Cytoplasmic male sterility in the Nicotiana sylvestris CMS mutants could be due either to gene deletion or to a regulatory effect of such a deletion on mitochondrial gene expression, rather than to the presence of specific polypeptides as has been shown in the T cytoplasm of maize, or in CMS Petunia.
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  • 4
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé La fièvre Méditerranéenne Familiale (FMF) est également connue sous le nom de Polysérite Héréditaire, une maladie présente dès la naissance chez les Arméniens, Turcs, les habitants des Balkans et les Juifs d'Afrique du Nord. Le diagnostic définitif de FMF est basé sur les données cliniques et l'histoire familiale puisqu'il n'y existe encore aucun test diagnostique spécifique. Un des principaux caractères cliniques de la maladie est la survenue de tableau de péritonite récidivante, qui ressemble, cliniquement et biologiquement, en tous points à une crise d'appendicite aiguë. Ceci explique pourquoi jusqu'à deux tiers des patients avec une FMF sont opérés en urgence. alors que leur appendice est le plus souvent normal. Comme l'appendicectomie laparoscopique a été démontrée comme une méthode súre, et pour éviter de faire un diagnostic erroné et éviter une chirurgie inutile, nous avons réalisé une appendicectomie laparoscopique chez 13 patients ayant une MFM dont les áges allaient de 8 à 32 ans. Tous les patients souffraient de leur MFM entre 1 et 12 ans (durée moyenne=3.8 ans) et présentaient une moyenne de 3.5 épisodes de tableaux de péritonites par an. Dans tous les cas, il a été possible de conclure l'intervention par laparoscopie sans avoir besoin de convertir en chirurgie ouverte. La durée moyenne de séjour à l'hôpital a eté de 3.07 jours. Il y a eu une seule complication: une infection superficielle de la plaie opératoire (7.6%) et la durée moyenne avant la reprise d'activité normale a été de 8.5 jours. Nous concluons que laparoscopie est utile et sûre chez le patient ayant une MFM. Elle permet d'exclure l'appendicite comme l'origine du tableau de péritonite chez ces patients, et aide ainsi à éviter une chirurgie inutile.
    Abstract: Resumen La Fiebre Mediterránea Familiar (FMF), también conocida como Poliserositis Hereditaria, es una enfermedad hereditaria que se encuentra comunmente en Armenios, Turcos, Arabes, Balcanos y Judíos originarios de las naciones del norte de Africa. El diagnóstico definitivo de la FMF se fundamenta en los hallazgos clínicos y en la historia familiar, puesto que aun no se ha hallado una prueba diagnóstica especifica. Entre sus principales características clínicas están los episodios agudos recurrentes de peritonitis. Durante cada episodio los hallazgos en el examen clínico y en las determinaciones de laboratorio pueden ser muy similares a los de la apendicitis aguda. Por consiguiente, hasta dos tercios de los pacientes con FMF son sometidos a apendicectomía de urgencia con el hallazgo de un apéndice normal en la mayoría de los casos. Puesto que la apendicectomía laparoscópica ha demostrado ser segura y ventajosa, y con miras a prevenir un error diagnóstico y una cirugía de emergencia innecesaria, hemos practicado la apendicectomia laparoscópica en 13 pacientes con FMF con edades entre 8 y 32 años. Estos pacientes sufrían la enfremedad entre 1 y 12 años (promedio 3.8), y presentaban un promedio de 3.5 episodios anuales de peritonitis por FMF. La totalidad de los procedimientos se completaron por laparoscopia y ninguno requirió conversión a cirugía abierta. El promedio de estancia postoperatoria fue de 3.07 días. La única complicación fue una infección superficial de herida en una paciente (7.6%) y el tiempo medio hasta la recuperación de actividad normal fue de 8.5 días. Nuestra conclusión es que la apendicectomía laparoscópica electiva en pacientes con FMF es un procedimiento seguro. Ayuda a excluir la apendicitis como causa de la peritonitis en los pacientes con FMF y puede prevenir una cirugía de emergencia innecesaria.
    Notes: Abstract Familial Mediterranean fever (FMF) also known as bereditary polyserositis, is an inherited disorder commonly found in Armenians, Turks, Arabs, Balkans, and Jews originating from North African countries. The diagnosis of FMF is based on clinical findings and family history, as no specific diagnostic test is yet available. One of its main clinical features is recurrent acute episodes of peritonitis. During such an episode, physical examination and laboratory findings may be similar to those for acute appendicitis. Therefore up to two-thirds of FMF patients undergo emergency appendectomy, with the appendix being normal in most cases. As laparoscopic appendectomy has proved to be safe and advantageous, and to prevent misdiagnosis and unnecessary emergency surgery, we performed elective laparoscopic appendectomy in 13 FMF patients ranging in age from 8 to 32 years. They had been suffering from the disease for 1 to 12 years (mean 3.8) and had had an average of 3.5 yearly episodes of FMF peritonitis. All procedures were concluded by laparoscopy without conversion to open surgery. The average postoperative hospital stay was 3.07 days. The only complication was superficial wound infection in one patient (7.6%), and the mean time to regain full normal activity was 8.5 days. We conclude that elective laparoscopic appendectomy in FMF patients is safe. It helps to exclude appendicitis as a cause for peritonitis in these patients and may prevent unnecessary emergency surgery.
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  • 5
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Cytoplasmic male sterility ; Cybrids ; Brassica ; Mitochondrial plasmid-like DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genomes of five rapeseed somatic hybrid plants, which combine in a first experimentBrassica napus chloroplasts and a cytoplasmic male sterility trait coming fromRaphanus sativus, and in a second experiment chloroplasts of a triazine resistantB. compestris and a cytoplasmic male sterility trait fromR. sativus, were analyzed by restriction endonucleases. Restriction fragment patterns indicate that these genomes differ from each other and from both parents. The presence of new bands in the somatic hybrid mitochondrial DNA restriction patterns is evidence of mitochondrial recombination in somatic hybrid cells. In both parental and somatic hybrid plants large quantitative variations in a mitochondrial plasmid-like DNA have been observed. Our results suggest that the cytoplasmic support for male sterility is located in the chromosomal mitochondrial DNA instead of the plasmid-like DNA.
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  • 6
    ISSN: 1432-0509
    Keywords: Intestinal lymphoma ; Hypertrophic osteoarthropathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Hypertrophic osteoarthropathy (HOA) in association with primary bowel disease is rare, but is usually seen in patients with chronic diarrheal states, such as Crohn's disease and ulcerative colitis. We record the first case of HOA associated with primary intestinal lymphoma in a patient who presented with chronic diarrhea.
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  • 7
    ISSN: 1432-0983
    Keywords: Key words Plant mitochondria  ;  nad3 duplication  ;   Amplification/recombination  ;  Gene co-transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Unlike other plant species, two copies of nad3 are present in Nicotiana sylvestris mitochondria. Both are localized downstream from an open reading frame (orf87 ), and are associated with either rps12 or the first exon of the nad1 gene. The orf87-nad3-nad1/A cluster is present in normal stoichiometry in Nicotiana tomentosiformis and is sub-stoichiometric in other Solanaceae, revealing recent amplification in the genus Nicotiana. It is suggested from sequence analysis that this cluster originated in an homologous recombination event that involved the nad3-rps12 intergenic region and the upstream region of an ancestral nad1 gene. Transcription patterns and RT-PCR showed that orf87-nad3-rps12 and orf87-nad3-nad1/A clusters are both co-transcription units.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Clinical rheumatology 12 (1993), S. 219-222 
    ISSN: 1434-9949
    Keywords: Temporal Arteritis ; Liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Giant cell arteritis is a vasculitis which usually affects large and medium-sized vessels in patients over 50 years old. The liver is one of the internal organs which can be involved in this systemic disease. During the last 15 years, 56 patients with giant cell arteritis were seen in our hospital. In 12 patients disturbed liver function tests were found. In the majority of cases the disturbance was of cholestatic type and resolved completely with steroid treatment. The association of temporal arteritis with disturbed liver function tests is discussed, with a review of the recent literature.
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  • 9
    ISSN: 1434-9949
    Keywords: Sjogren's Syndrome ; SSA/Ro Antibodies ; SSB/La Antibodies ; Saliva
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Autoantibodies to SSA/Ro and SSB/La antigens may have a pathogenic role in photosensitive skin disease and congenital complete heart block. Since salivary glands are the major target organ in Sjogren's syndrome (SS) we wondered whether these autoantibodies are present in saliva and may be involved in the sicca syndrome. Whole saliva and serum were collected from 15 patients with SS. Elisa analysis disclosed that 8 of the patients had anti-SSA/Ro antibodies, while 6 of them also had anti-SSB/La antibodies. Studies of immunoglobulin classes showed that the sera contained mainly IgG and IgM anti-SSA/Ro or SSB/La antibodies. One serum also contained IgA antibodies. Analysis of the saliva showed that in all positive samples IgG and IgA classes were present, while none of them contained IgM. Elisa and immunoblot analysis of sera and saliva from SLE patients without the sicca syndrome disclosed that both fluids contained anti-Sm antibodies. These findings suggest that the presence of anti-SSA/Ro and anti-SSB/La antibodies in saliva is not a unique phenomenon, characterizing the sicca syndrome. Therefore, their role in the pathogenicity of the Sjogren's syndrome has to be elucidated.
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  • 10
    ISSN: 1434-9949
    Keywords: Rheumatoid Arthritis ; Synacthen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of synthetic adrenocorticotrophic hormone (Synacthen), in conjunction with hydroxychloroquine, aurothioglucose, or pencillamine, was evaluated retrospectively in 21 patients with rheumatoid arthritis (RA). One mg of depo Synacthen was administered at increasing intervals of 4 to 14 days for a total period of 3 to 7 months. Fourteen patients with RA on either hydroxychloroquine or aurothioglucose and not on Synacthen, served as controls. Patients in the Synacthen group were, on the whole, sicker, as indicated by a lower functional capacity, higher mean erythrocyte sedimentation rate, and systemic and articular indices. Physicians' estimate of the patients condition after 1–2 months of therapy showed no improvement or deterioration in 10 out of 13 cases in the control group. Likewise, the erythrocyte sedimentation rate decreased significantly more and seronegativity was achieved in more of the Synacthen-treated cases. Six to 8 months after the beginning of therapy (1 to 4 months after cessation of Synacthen) clinical improvement was comparable in both groups, although seroconversion was more common in patients who had received Synacthen (7 out of 10 as compared to 1 out of 7 respectively). It is suggested that Synacthen may be used safely in the early phase of selected RA patients, until the effect of second-line drugs is achieved.
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