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  • 1
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cloning and sequence of the regulatory region of the threonine operon of E. coli K12 carried by a constitutive mutant are presented. In this mutant the transcription termination signal, located upstream from the first structural gene, is deleted. These results strongly support the model of attenuation for the regulation of the threonine operon as first proposed by Gardner (1979)
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir haben in Kulturüberständen einer Tn1545-induzierten, nicht-hämolysierenden Mutante vonListeria monocytogenes mittels Immunblotting mit Antiserum gegen gereinigtes Listeriolysin ein Eiweißmolekül von 52000D entdeckt (das sezernierte Listeriolysin O hat ein Molekulargewicht von 60000D). Die Insertionsstelle des Transposon wurde kloniert und einer Sequenzanalyse unterworfen. Das Transposon hatte an einem offenen Ableseraster (ORF) inseriert. Zwischen diesem ORF, Streptolysin O und Pneumolysin wurden Homologien festgestellt, die zeigen, daß das Transposon tatsächlich am Listeriolysin-Gen inseriert hatte. Da die nicht-hämolysierende Mutante avirulent war, konnten wir mit unserer Arbeit nachweisen, daß für die Virulenz die Anwesenheit des Listeriolysin-Gen oder seiner angrenzenden Region erforderlich ist.
    Notes: Summary In culture supernatants of a Tn 1545-induced non-hemolytic mutant ofListeria monocytogenes, by immunoblotting with an anti-serum raised against purified listeriolysin O, we have detected the presence of a truncated protein of 52,000D (the secreted listeriolysin O is 60,000D). The region of insertion of the transposon has been cloned and sequenced. The transposon had inserted in an open reading frame. The homologies detected between this ORF, streptolysin O and pneumolysin demonstrate the the transposon had indeed inserted in the listeriolysin O gene. As the non-hemolytic mutant was non-virulent, our work demonstrated that a genetic determinant essential for virulence is the listeriolysin O gene or its adjacent region.
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