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  • 1
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Die Mikrochromatographie von dansylierten Verbindungen wurde angewandt, um die Verteilung von Aminen und Aminosäuren in den Retzius-Zellen und der Bauchganglienkette des BlutegelsHirudo medicinalis zu untersuchen. Die Retzius-Zellen weisen sehr grosse Mengen an Serotonin, 5-HTP und Tryptophan auf. Ihr Gehalt an Asparagin- und Glutaminsäure ist ebenfalls, allerdings in geringerem Masse, grösser als im umgebenoen Nervengewebe, während Histidin, Prolin, Valin, Alanin, Glycin sowie Threonin, Glutamin und Asparagin in geringeren Mengen vorkommen.
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  • 2
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Sowohl Nervengewebe als auch definierte Nervenzellsomata (GSC) vonHelix pomatia können zwar14C-Serotonin aufnehmen, aber nur im Nervengewebe wird es zu14C-Hydroxyindolessigsäure umgewandelt. Die Wirkung von Imipramin und Nialamid macht wahrscheinlich, dass Serotonin auf zwei Wegen inaktiviert wird, d. h. durch enzymatische Oxydation und durch Wiederaufnahme in die synaptischen Boutons.
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  • 3
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Injection of 5,7-dihydroxytryptamine into the substantia nigra of rats produces an increase of dopamine in the ipsilateral striatum, and when these animals are injected with amphetamine they do not exhibit any rotation. The mode of action of this neurotoxin is compared with that of 6-hydroxydopamine.
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  • 4
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Von den im Schneckenhirn (Helix pomatia) wahrscheinlich als Neurotransmitter wirkenden Substanzen führen Dopamin und Acetylcholin zu einem erhöhten Einbau von32P in Phospholipide; Serotonin zeigt eher einen gegenteiligen Effekt und Noradrenalin bleibt ohne Einfluss. Phosphatidylinositol weist die höchste Einbaurate auf. Die Resultate unterstreichen die Bedeutung von Dopamin, Acetylcholin und Serotonin als Neurotransmitter im Schneckenhirn und deren Einfluss auf den Metabolismus von Membranphospholipiden.
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  • 5
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and characterization of MAO in various tissues of the snail were analyzed. Only low amounts of the enzyme exist in the different tissues and data suggest that there is more than one type of MAO.
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  • 6
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A pteridine containing nucleotide has been isolated from kidney extracts of rats treated with Triamterene (2,-4,-7-triamino-6-phenyl-pteridine). In addition to 1 mol triamterene 1 mol adenosine, approximately 1 mol ribose and 3 mol phosphate have been found to constitute the compound. It is suggested that the nucleotide has been synthetized in vivo from NADP by enzymic exchange of nicotinamide for triamterene.
    Notes: Zusammenfassung Ein pteridinhaltiges Nucleotid wurde aus den Nieren von Ratten extrahiert, die mit Triamteren (2,-4,-7-triamino-6-phenylpteridin) behandelt wurden. Die Verbindung enthält außer 1 Mol Triamteren ca. 1 Mol Ribose und 3 Mol Phosphat. Wahrscheinlich ist das Nucleotid durch enzymatischen Austausch des Nicotinamids gegen Triamteren aus dem NADP entstanden.
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  • 7
    ISSN: 1432-0878
    Keywords: GABA ; Immunocytochemistry ; Neurones ; Retina ; Different species ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localisation of GABA immunoreactive neurones in retinas of a variety of animals was examined. Immunoreactivity was associated with specific populations of amacrine neurones in all species examined, viz. rat, rabbit, goldfish, frog, pigeon and guinea-pig. All species, with the exception of the frog, possessed immunoreactive perikarya in their retinal ganglion cell layers. These perikarya are probably displaced amacrine cells because GABA immunoreactivity was absent from the optic nerves and destruction of the rat optic nerve did not result in degeneration of these cells. GABA immunoreactivity was also associated with the outer plexiform layers of all the retinas studied; these processes are derived from GABA-positive horizontal cells in rat, rabbit, frog, pigeon and goldfish retinas, from bipolar-like cells in the frog, and probably from interplexiform cells in the guinea-pig retina. The development of GABA-positive neurones in the rabbit retina was also analysed. Immunoreactivity was clearly associated with subpopulations of amacrine and horizontal cells on the second postnatal day. The immunoreactivity at this stage is strong, and fairly well developed processes are apparent. The intensity of the immunoreactivity increases with development in the case of the amacrine cells. The immunoreactive neurones appear fully developed at about the 8th postnatal day, although the immunoreactivity in the inner plexiform layer becomes more dispersed as development proceeds. The immunoreactive horizontal cells become less apparent as development proceeds, but they can still be seen in the adult retina. The GABA immunoreactive cells in rabbit retinas can be maintained in culture. Cultures of retinal cells derived from 2-day-old animals can be maintained for up to 20 days and show the presence of GABA-positive cells at all stages. In one-day-old cultures the GABA immunoreactive cells lacked processes but within three days had clearly defined processes. After maintenance for 10 days a meshwork of GABA-positive fibres could also be seen in the cultures.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular histology 13 (1981), S. 207-225 
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 9
    ISSN: 1618-2650
    Keywords: Analyse von Aminosäuren ; Chromatographie, Dünnschicht ; automat. F luorimetrie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 10
    ISSN: 1570-7458
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary High numbers of simuliid bites may cause allergic reactions or even death in cattle due to the injection of salivary gland secretions. By means of micro-electrophoresis the proteins of the salivary glands and the hemolymph of three black fly species Boophthora erythrocephala, Odagmia ornata and Wilhelmia lineata were fractionated in polyacrylamide gel gradients in 10 μl capillaries. The molecular weight of the proteins was determined. The microtechnique applied allows a fractionation of salivary gland proteins from a single animal. Identical protein patterns mainly with relatively high molecular proteins (MW〉69000) were found in the spherical salivary glands in males and in the so-called accessory gland in females which has the same histological structure. The tube-shaped main gland which is only found in bloodsucking females contains several low molecular proteins (MW〉6000〈69000). These proteins are found neither in male nor in female hemolymph. These observations support the assumption that the females produce a specific type of salivary secretion in their main glands. During bloodsucking 50%–70% of the soluble salivary proteins are injected into the host animal. The recharging of the glands takes 4–5 hr. Proteins from the accessory glands show a very similar pattern to those of the hemolymph, but not to those in the main glands. This suggests a close relationship between the two sites. Membrane-bound proteins of the salivary glands can be fractionated as well on micro gel gradients after SDS charging. They differ from the water-soluble proteins. The ratio of these two types of proteins is 2:1. The proteins of a single salivary gland or parts of it can be fractionated without loss of material after complete solubilisation in SDS in a capillary directly on top of the separation gel.
    Notes: Zusammenfassung Kontinuierliche Gradienten-Gele in μl Kapillaren wurden zur Fraktionierung von wasserlöslichen und membranständigen Proteinen aus Speicheldrüsen und Hämolymphe von drei Simuliidenarten Boophthora erythrocephala (De Geer), Odagmia ornata (Meigen) und Wilhelmia lineata (Meigen) verwendet. Speicheldrüsen von Männchen und Weibchen haben jeweils charakteristische für die einzelne Art spezifische Bandenmuster. Das Proteinmuster der weiblichen Hauptdrüse unterscheidet sich deutlich von dem der Nebendrüse, das nahezu identisch ist mit dem Proteinmuster der männlichen Drüse. Die Hauptdrüse der Weibchen enthält im wesentlichen relativ neidermolekulare Proteine (〉6000〈69000); bei der Blutaufnahme werden 50%–60% von Haupt-und Nebendrüsenproteinen in das Wirtstier injiziert. Die Wiederauffüllung der Drüsen ist nach 4–5 Stunden abgeschlossen. Einige Proteine der Nebendrüse haben einen identischen Rf-Wert mit bestimmten Hämolymphproteinen. Dies spricht für eine Aufnahme von Hämolymphproteinen in die Drüse. Es wird gezeigt, daß eine einzelne Drüse mit einem Proteingehalt von ca. 3 μg für eine Fraktionierung ausreicht, wenn die Drüse direkt über dem Gel mit SDS inkubiert wird.
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