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  • 1
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Des cellules de cartilage de croissance de jeunes lapins ont été cultivées in vitro et leurs homogénats ont été injectés à la souris. Des hybridomes ont été préparés par la technique de fusion des cellules de myélome et des cellules de la rate de souris immunisée. Des anticorps monoclonaux (MoAbs) ont été produits par les hybridomes dans la cavité péritonéale de la souris et certains d'entre eux, provisoirement appelés MoAbs A, B, D, N, P et S, ont été étudiés. La localisation des antigènes de chaque MoAbs dans le cartilage de croissance ou dans le cartilage au repos avoisinant a été recherchée par coloration fluorescente indirecte des anticorps. Le poids moléculaire des antigènes a été évalué par coloration immunogène après électrophorèse au gel polycrylamide SDS. Le MoAb A et le MoAb N ont coloré les cellules du cartilage au repos et les cellules du cartilage de croissance à l'exception du cartilage de croissance calcifié. Le MoAb B a coloré le cartilage de croissance hypertrophique et calcifié ainsi que les matrices du cartilage au repos et le cartilage de croissance proliférant. Le MoAb D a coloré le cartilage de croissance calcifié. Le MoAb P et le MoAb S ont coloré les cellules du cartilage au repos et les matrices dans le cartilage de croissance, de manière particulièrement intensive dans le cartilage de croissance hypertrophique et le périchondre. Le poids moléculaire de l'antigène des MoAbs A, P et S s'est trouvé être respectivement 40–70 KD, 35–40 KD et 30 KD.
    Notes: Summary Growth cartilage (GC) cells of young rabbits were cultured in vitro and their homogenates were injected into mice. Hybridomas were prepared by the cell fusion technique between the myeloma cells and the spleen cells of the immunized mice. Monoclonal antibodies (MoAbs) were produced by the hybridomas in the peritoneal cavities of the mice, and some of these, temporarily named MoAbs A, B, D, N, P, and S, were studied. The localization of the antigens of each of the MoAbs in the GC or adjacent resting cartilage (RC) was examined by indirect fluorescent antibody staining. The molecular weight of the antigens was examined by immunoblot staining after SDS-polyacrylamide gel electrophoresis: MoAb A and MoAb N stained RC cells and GC cells, except calcified GC. MoAb B stained the hypertrophic and calcified GC, and matrices in the RC and proliferating GC. MoAb D stained the calcified GC. MoAb P and MoAb S stained the RC cells and the matrices in the GC, intensively in the hypertrophic GC and perichondrium. The molecular weights of the antigens of MoAbs A, P, and S were 40–70 KD, 35–40 KD and 30 KD, respectively.
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  • 2
    ISSN: 1432-5411
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The reaction was studied experimentally at 60 MeV. Angular dependences of the cross sections and the analyzing powers for the quasi-free scattering resembled those for freed-p scattering. The experimental results were compared with a Born approximation calculation on the basis of the three-body scattering theory by Alt, Grassberger, and Sandhas.
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  • 3
    ISSN: 1434-601X
    Keywords: PACS: 24.50.+g Direct reactions – 25.60.Gc Breakup and momentum distributions – 27.20.+n 6 ≤ A ≤ 19
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: Elastic scattering and inclusive breakup of 6Li particles on 12C, 58Ni, 90Zr, and 208Pb targets are measured at 100A MeV. The elastic scattering data are compared with single channel and Coupled Discretized Continuum Channels calculations. The coupling-effect between the elastic and the breakup channels is important even at an incident energy of 100A MeV and cannot be neglected. The inclusive breakup data are investigated for orbital dispersion effects which are found to be less significant at 100A MeV. The longitudinal momentum distributions are broader than predicted by theoretical expectations.
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  • 4
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Eighteen protein amino acids with milk casein composition were heated in a modified sea medium. Marigranules were formed in the precipitates and soluble polymers were formed in the supernatant. Time course of the reaction (ultraviolet spectra, the concentration of metal ions, and the concentration of amino acids in the supernatant) were measured. The time course of the formation of the soluble polymers was also studied by Bio-Gel P-2 column. High molecular weight soluble polymers (HMWSP) were separated from low molecular weight ones by dialysis. It was shown that these polymers catalyzed the dehydrogenation of NADH. These polymers also catalyzed the coupled reaction between dehydrogenation of NADH and reduction of resazurin. This coupled reaction was accelerated by the light.
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  • 5
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Functions of coacervate droplets as protocells are studied by using synthetic polymers. The coacervate droplets were made from PVA-A and PVA-S. When glycine or diglycine were in the surrounding medium, the coacervate droplets concentrated them. The concentration of glycine in the coacervate droplets was higher than that of diglycine. When this mixture was irradiated by UV light, the coacervate droplets protected them from the photochemical decomposition.
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  • 6
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract From our knonwledge of present day organisms, it is hard to imagine a living assembly, even at its most primitive stage, without macromolecules. In order to look for the macromolecules which possibly participated in the assembly of the primitive organisms, the reaction and formation of polymers in HCN under irradiation of ultraviolet ray of 184.9 nm. was studied. As a example of a simple way of producing an assembly of macromolecules, the mechanism of coacervation was studied by using gelatin as the material.
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  • 7
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract A mixture of eighteen protein amino acids was heated in sea water medium enriched with transition metal ions. Small granules were obtained as precipitates. Both dialyzable polymers and undialyzable polymers were obtained from the supernatant. Dialyzable polymers yielded mainly Glu, Asp, Ser, and Thr on hydrolysis; undialyzable polymers (C, 29.45; H, 3.87; N, 4.87; and ash, 31.5 wt%) yielded Thr, Asp, Glu, Gly, Leu, Ser, Lys, Pro, His, Phe, and a few unidentified ninhydrin positive peaks after acid hydrolysis. Five wt% of the undialyzable polymers consist of acid-hydrolyzable protein amino acids.
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  • 8
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoclonal antibodies (mAbs) were raised by injection of a homogenate of cultured growth cartilage (GC) cells from young rabbit ribs. These mAbs were examined by immunohistochemical staining for their reactivity to paraffin sections of rabbit tissues. The results showed that an mAb reacted preferentially with late hypertrophic and calcified costal GC zones. The mAb also reacted with hypertrophic GC adjacent to bone that existed in sternum and femur, but not to other cartilages, including resting cartilage, articular cartilage, auricular cartilage, nasal cartilage, tracheal cartilage and meniscus cartilage, or with other tissues, including tendon, skin, muscles, lung, liver, heart, thymus, spleen, eye and gut. It reacted with a wider area of the GC zone when the sections were decalcified, although its reactivity with the extended area was much less intensive than that with late hypertrophic and calcified GC zones. On treatment of the sections with bacterial collagenase, neither the reactive area nor its intensity were changed, while when treated with trypsin the reactivity was lost. These results suggest the existence of a certain molecule which distinguishes GC (osteogenic cartilage) from other (non-osteogenic) cartilage. This mAb is a useful probe for distinguishing osteogenic cartilage from non-osteogenic cartilage, and for studying differentiation steps of cartilage cells in endochondral bone formation. The mAb can also be used as a probe for clinical and stored specimens because it reacts with decalcified and paraffin-embedded human specimens.
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