Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: Life sciences ; Plant Biochemistry ; Plant Ecology ; Plant anatomy ; Plant development ; Plant breeding ; Life sciences ; Plant Anatomy/Development ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Biochemistry ; Plant Ecology ; Springer eBooks
    Description / Table of Contents: Part I: Orchid Seeds in Plant Propagation and Conservation -- Understanding Seed and Protcorm Development in Orchids.-℗ Orchid Mycorrhizal Fungi:℗ Isolation and Identification Techniques -- Protocols for Establishing Fungi-Protocorm Culture -- Orchid Seed and Pollen: A Tool-kit for Long-term Storage, Viability Assessment and Conservation -- Part II: Orchid Micropropagation -- Orchid Seed Germination and Micropropagation I: Background information and Related Protocols -- Orchid Seed Germination and Micropropagation II: Media information and Composition -- Orchid Micropropagation: An Overview of Approaches and Methodologies -- Common Protocols in Orchid Micropropagation -- Micropropagation of Orchids by Using Bioreactor Technology -- Enhanced Growth and Development of Cymbidium and Phalaenopsis Plantlets Cultured in vitro Under Light-emitting Diodes -- Part III: Special Techniques -- Tracking Pollen Fates in Orchid Populations -- Cut-column Pollination Method to Overcome Pollination Barrier in Phalaenopsis -- In vitro Flowering and Breeding of Erycina pusilla -- Virus Elimination through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System -- Cryopreservation -- Haploid Seed Formation via Parthenogenesis in Bletilla -- A Protocol for the Induction of Polyploids in Phalaenopsis Orchids by in vitro Method without Using Anti-microtubule Agents -- Virus Detection -- Part IV: Cell and Molecular Biology and Transformation -- Genetic Transformation on Orchid species: An Overview of Approaches and Methodologies -- Recent Protocols on Genetic Transformation of Orchid Species -- The Use of Laser Microdissection to Investigate Cell Specific Gene Expression in Orchid Tissues -- Part V: Orchid Propagation and Maintenance -- Vegetative Propagation of Orchids -- Orchid Cultivation in Taiwan: Conventional and Innovative Methods -- Strategies in Orchid Health Maintenance -- Part VI: Showcasing Orchids -- Macro Flower Photography: An Introduction to Photographic Principles and Techniques -- An Introduction to Chinese Orchid Paintings -- Recent Advances in Orchid Seed Germination and Micropropagation
    Abstract: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance.℗ This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing. This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation.℗ The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an℗ in vitro℗ environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for on這s enjoyment
    Pages: XI, 524 p. 151 illus., 134 illus. in color. : online resource.
    ISBN: 9781493977710
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    facet.materialart.
    New York, NY : Springer New York
    Keywords: Medicine ; Immunology ; Biomedicine ; Immunology ; Springer eBooks
    Description / Table of Contents: A Fungal Protease Model To Interrogate Allergic Lung Immunity -- Use of the Litomosoides sigmodontis Infection Model of Filariasis to study Type 2 Immunity -- Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool With New Clinical Applications -- A Mouse Model of Peanut Allergy Induced By Sensitization through the Gastrointestinal Tract -- Induction and Characterization of the Allergic Eye Disease Mouse Model -- Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung -- In vitro and In vivo IgE/Antigen Mediated Mast Cell Activation -- The use of Human and Mouse Mast Cell and Basophil Cultures to Assess type-2 Inflammation -- Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation -- Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors -- Characterization of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry -- Characterization of Mouse gd T Cell Subsets in the Setting of type-2 Immunity -- The Identification of Allergen-derived T cell Epitopes -- Generation of Allergen-specific Tetramers for a Murine Model of Airway Inflammation -- The Generation and Use of Allergen-specific TCR Transgenic Animals -- Using Cytokine Reporter Mice to Visualize Type-2 immunity in vivo -- Live Imaging of IL-4 expressing T Follicular Helper Cells in Explanted Lymph Nodes -- Imaging Precision Cut Lung Slices to Visualize Leukocyte Localization and Trafficking -- Study of IgE-producing B cells using the Verigem Fluorescent Reporter Mouse -- Chromatin Preparation from Murine Eosinophils for Genome-wide Analyses -- A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers -- An Integrated and Semi-automated Micro-scaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq for Large-scale Epigenetic Studies -- Library preparation for ATAC-sequencing of Mouse CD4+ T Cells Isolated from the Lung and Lymph Nodes after Helminth Infection -- Identification of Functionally-relevant microRNAs in the Regulation of Allergic Inflammation -- The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation -- Assessing the Mouse Intestinal Microbiota in Settings of type-2 Immune Responses -- The use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology -- A Consistent Method to Identify and Isolate Mononuclear Phagocytes from Human Lung and Lymph Nodes -- Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation -- Utilization of Air-liquid Interface Cultures as an in vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin 13
    Abstract: This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside
    Pages: XVI, 434 p. 80 illus., 53 illus. in color. : online resource.
    ISBN: 9781493978960
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    Keywords: Life sciences ; Plant Biochemistry ; Plant Ecology ; Plant anatomy ; Plant development ; Plant breeding ; Life sciences ; Plant Anatomy/Development ; Plant Systematics/Taxonomy/Biogeography ; Plant Breeding/Biotechnology ; Plant Biochemistry ; Plant Ecology ; Springer eBooks
    Description / Table of Contents: Part I: Orchid Seeds in Plant Propagation and Conservation -- Understanding Seed and Protcorm Development in Orchids.-℗ Orchid Mycorrhizal Fungi:℗ Isolation and Identification Techniques -- Protocols for Establishing Fungi-Protocorm Culture -- Orchid Seed and Pollen: A Tool-kit for Long-term Storage, Viability Assessment and Conservation -- Part II: Orchid Micropropagation -- Orchid Seed Germination and Micropropagation I: Background information and Related Protocols -- Orchid Seed Germination and Micropropagation II: Media information and Composition -- Orchid Micropropagation: An Overview of Approaches and Methodologies -- Common Protocols in Orchid Micropropagation -- Micropropagation of Orchids by Using Bioreactor Technology -- Enhanced Growth and Development of Cymbidium and Phalaenopsis Plantlets Cultured in vitro Under Light-emitting Diodes -- Part III: Special Techniques -- Tracking Pollen Fates in Orchid Populations -- Cut-column Pollination Method to Overcome Pollination Barrier in Phalaenopsis -- In vitro Flowering and Breeding of Erycina pusilla -- Virus Elimination through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System -- Cryopreservation -- Haploid Seed Formation via Parthenogenesis in Bletilla -- A Protocol for the Induction of Polyploids in Phalaenopsis Orchids by in vitro Method without Using Anti-microtubule Agents -- Virus Detection -- Part IV: Cell and Molecular Biology and Transformation -- Genetic Transformation on Orchid species: An Overview of Approaches and Methodologies -- Recent Protocols on Genetic Transformation of Orchid Species -- The Use of Laser Microdissection to Investigate Cell Specific Gene Expression in Orchid Tissues -- Part V: Orchid Propagation and Maintenance -- Vegetative Propagation of Orchids -- Orchid Cultivation in Taiwan: Conventional and Innovative Methods -- Strategies in Orchid Health Maintenance -- Part VI: Showcasing Orchids -- Macro Flower Photography: An Introduction to Photographic Principles and Techniques -- An Introduction to Chinese Orchid Paintings -- Recent Advances in Orchid Seed Germination and Micropropagation
    Abstract: The orchid family is one of the largest families of flowering plants known for their beauty and economic importance.℗ This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing. This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation.℗ The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an℗ in vitro℗ environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for on這s enjoyment
    Pages: XI, 524 p. 151 illus., 134 illus. in color. : online resource.
    ISBN: 9781493977710
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    facet.materialart.
    New York, NY : Springer New York
    Keywords: Medicine ; Human Genetics ; Biomedicine ; Human Genetics ; Springer eBooks
    Description / Table of Contents: Clearance of Maternal RNAs: Not a Mummy’s Embryo Anymore -- Link of Zygotic Genome Activation and Cell Cycle Control -- Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis -- Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR -- Detection of miRNA in Mammalian Oocytes and Embryos -- Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method -- Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation -- Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos -- RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos -- Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry -- Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo -- Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos -- Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation -- Targeted Gene Knockdown in Early Embryos using siRNA -- Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput -- CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine -- Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development -- Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes
    Abstract: This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA
    Pages: XI, 272 p. 51 illus., 41 illus. in color. : online resource.
    ISBN: 9781493969883
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...