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  • Dorsal closure  (1)
  •  Marker-aided selection   (1)
  • Basement membranes
  • 1995-1999  (2)
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Year
  • 1
    ISSN: 1432-2242
    Keywords: Key words Genetic map  ;  Marker-aided selection  ; Oryza sativa  ;  RFLP markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with AFLP markers they will be very useful for marker-assisted backcrossing.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-041X
    Keywords: Type IV collagen ; Drosophila ; Germ band retraction ; Dorsal closure ; Nerve cord condensation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A collagen gene (Dcg1) was characterized in Drosophila melanogaster and shown to encode a peptide related to vertebrate basement membrane type IV collagen chains. To study the function of type IV collagen during Drosophila development, we transformed flies with a partially truncated Dcg1 gene under the control of a heat-shock promotor. This construct induced synthesis of shortened pro-α chains which associated with normal ones and thereby caused degradation of the shortened and normal pro-α chains through a process called “pro-collagen suicide”. A large proportion of embryos expressing the transgene developed a phenotype exhibiting absence or partial retraction of the germ band with defects in nerve cord condensation and dorsal closure. Together these results indicated that, during embryogenesis, type IV collagen was an essential guiding factor for cell-matrix interactions in morphogenetic events.
    Type of Medium: Electronic Resource
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