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  • (Acremonian)  (1)
  • Dextran-modified poly(methyl methacrylate) latex particles  (1)
  • 1
    ISSN: 0167-4838
    Keywords: (Acremonian) ; Enzyme characterization ; Enzyme purification ; Fungus ; Glucooligosaccharide oxidase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-1536
    Keywords: Key words Isothermal equilibrium adsorption ; Concanavalin A ; Dextran-modified poly(methyl methacrylate) latex particles ; Electrostatic/affinity interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Isothermal equilibrium adsorption experiments were carried out to study the adsorption of concanavalin A (Con A) on dextran-modified poly(methyl methacrylate) (PMMA) latex particles. Three PMMA particles with various levels of dextran modification were selected for study: 0% (designated as D0), 1.24% (D20), and 2.45% (D75) based on total polymer weight. The Langmuir model is applicable to both D0 and D20 systems, although the data for the D20 system are somewhat scattered. On the other hand, the amount of Con A adsorbed per gram polymer particles (q*) versus the Con A concentration in water (c*) curve for the D75 system cannot be described by the Langmuir model. The deviation is attributed to the formation of a crosslinked network structure, caused by specific binding of the dimeric Con A molecules onto two neighboring particles with grafted dextran. The ratio of the initial number of Con A molecules to the initial number of active binding sites on the dextran-modified particle surface plays an important role in determining the structure of flocs formed. The maximum amount of Con A adsorbed on the particle surface (q max) is of the order of 10−1 μmol per gram particles and q max in decreasing order is D75 〉 D20 〉 D0. The dissociation constant of the Con A-D20 (or Con A-D75) pair is of the order of 10−1 μmol dm−3 which is 1 order of magnitude smaller than that of the Con A-D0 pair. Thus, the electrostatic interaction between Con A and D0 is much weaker than the affinity interaction between Con A and D20 (or D75). An empirical model is proposed to qualitatively explain the q* versus c* data.
    Type of Medium: Electronic Resource
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