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  • cDNA  (2)
  • 33.70.Jg  (1)
  • 3H  (1)
  • AC-ECD  (1)
  • Springer  (5)
  • Munksgaard International Publishers
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Publisher
  • Springer  (5)
  • Munksgaard International Publishers
Years
  • 1
    ISSN: 1573-4919
    Keywords: cDNA ; human ; placenta ; immunoglobulin ; pregnancy-specific β1-glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Three cDNAs encoding members of the pregnancy-specific β1-glycoprotein (PSG) family were isolated from human term placental cDNA library. All three cDNAs encode proteins with similar domain structure. There is a leader sequence of 34 amino acids followed by an N-domain of 109 amino acids. Immediately after the N-domain are one or two copies of a repeating A-domain of 93 amino acids, a B-domain of 85 amino acids and a C-domain of variable size. The proteins are highly hydrophilic. However, one of them has an 81-amino acid C-domain which is very hydrophobic and could potentially serve as a membrane attachment site. The putative cell-cell recognition tripeptide, Arg-Gly-Asp, is present in the N-domain of two of the proteins. Partial sequence of one of the cDNAs has been found in HeLa cells while cDNAs highly homologous to two of the cDNAs have been found in the fetal liver. Functional roles of the PSG proteins basing on their structure are proposed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-6036
    Keywords: 36.40.+d ; 33.70.Jg ; 67.40.Yv
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We examine the effects of impurities (“doping”) and rotational excitation on the structural and energetic properties of helium clusters. Quantum Monte Carlo (QMC) techniques are used to study ground and rotationally excited states of pure and doped clusters. We use exponentially correlated wave functions and treat the molecular impurities as rigid. Whereas pure HeN show essentially monotonic decay of density from a central maximum value, addition of impurities induces local ordering of He to an extent dependent on the impurity-He binding. Rotational excitation of HeN gives rise to extremely large centrifugal distortions. The location of impurities also appears to change upon rotational excitation. The implications of these distortions on impurity spectra are discussed for SF6HeN, and compared to recent experimental results.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Microchimica acta 96 (1988), S. 11-26 
    ISSN: 1436-5073
    Keywords: electron capture detector (ECD) ; electron oscillation ; AC-ECD ; argon-methane ; non-homogeneous ECD kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The oscillation of electrons—as driven by AC polarization—can be used to extract a high-sensitivity signal from a conventional electron capture detector. For example, hexachloroethane was detected at a hypercoulometric ratio of 2.0 F/mol, down to ca. 60 fg/s (S/N=3) and with a linear range in excess of two orders of magnitude. The change in carrier gas from nitrogen to argon-methane produced the expected order-of-magnitude increase in optimum oscillation frequency. Anab initio simulation of potentials and ion populations in a heterogeneous electron capture system under a high-frequency AC regime provided further insight into the detector's mechanism: Hypercoulometric response is mainly caused by increased cation-electron recombination in the plasma region, owing to a decreased field gradient and an increased cation concentration.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1436-5073
    Keywords: ionization topography ; 3H ; 63Ni ; electron capture detector
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The ion-pair generation rate (ionization topography) in plasmas from63Ni and particularly Ti3H4 foils, as used in electron capture detectors, was measured at room temperature using large, parallel plates of low backscattering ability in nitrogen gas of varying density. For one atmosphere pressure, the fall-off of ion pair formation as calculated from the exponential region equalsN 0·e −0.19d for63Ni andN 0·e −1.4d for3H (whereN 0 is the initial ionization rate immediately adjacent to the foil andd is the distance from the foil in mm). The experimentally measured half ranges (distances from the foil within which 50% of all possible ion pairs are created) are 2.7 mm for63Ni and 0.27 mm for3H. The half ranges calculated from the exponential region where there is less interference from electron backscattering, are 3.7 and 0.5 mm, respectively. The latter values are considered closer to the “true”, unimpeded ionization topography near planar63Ni and3H foils.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2048
    Keywords: Key words: Ascorbate peroxidase ; cDNA ; Glycine (ascorbate peroxidase)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Screening of a cDNA library from soybean (Glycine max (L.) Merr. cv. Century) with probes based upon cytosolic ascorbate peroxidase (APx; EC 1.11.1.11) genes identified two full-length clones (SOYAPx1, SOYAPx2) apparently encoding for different soybean leaf cytosolic APxs. The deduced amino acid sequences of the two APx cDNA products differed in 13 of the 250 amino acids. The SOYAPx1 cDNA was identical to the cytosolic APx cDNA previously found in soybean root nodules. Escherichia coli expression systems were developed using both soybean APx cDNAs. Recombinant SOYAPx1 and SOYAPx2 were then utilized to characterize the enzymatic properties of the two APx cDNA products.
    Type of Medium: Electronic Resource
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