Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • AMPLIFIES INFLAMMATION  (1)
  • 1
    Keywords: RECEPTOR ; CELLS ; EXPRESSION ; CELL ; Germany ; human ; TYROSINE KINASE ; PROTEIN ; RNA ; cell line ; LINES ; TRANSDUCTION ; ACTIVATION ; COMPLEX ; RESPONSES ; COMPLEXES ; CUTTING EDGE ; DENDRITIC CELLS ; CELL-LINES ; PHOSPHORYLATION ; signal transduction ; SIGNAL ; MOUSE ; PATTERNS ; SIGNAL-TRANSDUCTION ; CELL-LINE ; LINE ; B-CELLS ; NATURAL-KILLER-CELLS ; NK cells ; cell lines ; REGULATOR ; NEUTROPHILS ; signaling ; RE ; PRODUCTS ; INTERFERENCE ; RNA INTERFERENCE ; LEADS ; CYTOKINE PRODUCTION ; LEVEL ; SIGNALS ; B-CELL ; immunology ; DAP12 ; AMPLIFIES INFLAMMATION ; PHOSPHOLIPASE C-GAMMA-1
    Abstract: The engagement of triggering receptor expressed on myeloid cells 1 (TREM-1) on macrophages and neutrophils leads to TNF-alpha and IL-8 production and enhances inflammatory responses to microbial products. For signal transduction, TREM-1 couples to the ITAM-containing adapter DNAX activation protein of 12 kDa (DAP12). In general, ITAM-mediated signals lead to cell activation, although DAP12 was recently implicated in inhibitory signaling in mouse macrophages and dendritic cells. To date, signals downstream of the TREM-1 and DAP12 complex in myeloid cells are poorly defined. By analyzing receptor-induced tyrosine phosphorylation patterns, we discovered that the ligation of TREM-1 leads to tyrosine phosphorylation of the non-T cell activation linker (NTAL; also called linker of activation in B cells or LAB) in a myelomonocytic cell line and primary human granulocytes. Using RNA interference to decrease the expression levels of NTAL, we demonstrate that in NTAL knockdown cell lines the phosphorylation of ERK1/2 is enhanced. In addition, low levels of NTAL are correlated with decreased and delayed mobilization of Ca2+ after TREM-1 triggering. Most importantly, we demonstrate that NTAL acts as a negative regulator of TNF-alpha and IL-8 production after stimulation via TREM-1. Our results show that activation signals delivered via DAP12 can be counterbalanced by the adaptor NTAL, identifying NTAL as gatekeeper of TREM-1/DAP12-induced signaling in myeloid cells. The Journal of Immunology, 2007, 178: 1991-1999
    Type of Publication: Journal article published
    PubMed ID: 17277102
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...