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  • Analytical Chemistry and Spectroscopy  (2)
  • ATPase  (1)
  • United States
  • 1985-1989  (3)
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Year
  • 1
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The ratio of the fragment ions at m/z 122 and m/z 123 in the positive ion fast atom bombardment or secondary ion mass spectra of thiamine hydrochloride varies with sample preparation and experimental conditions. For all mass spectra that contained significant abundances of matrix (S) ions [S + H]+, the fragment at m/z 123 is the more abundant of the two ions. If [S + H]+ ions are not observed in the mass spectrum under the conditions selected, the ion at m/z 122 is more abundant. This correlation suggests that hydrogen transfer to the fragment ion occurs in the gas phase, with the composition of the ion-solvent cluster ions in the selvedge region being the key factor. The ratio of the fragment ions at m/z 123 and m/z 122 is thus an indicator of the extent of protonation in the selvedge, the region immediately above the solvent surface created by primary particle bombardment.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0886-1544
    Keywords: dynein ; erythro-9-[3-2-(hydroxynonyl)]adenine (EHNA) ; ATPase ; inhibition ; axoneme ; cytoplasm ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In current purification strategies, affinity for microtubules or calmodulin is used to identify and purify cytoplasmic dynein-like ATPase from cell-free extracts of unfertilized sea urchin eggs. However, affinity purification procedures, though they define dynein-like ATPase activity, have not yet proven to be quantitative. An alternative purification strategy capable of producing a high yield of enzyme would require a specific assay in order to monitor cytoplasmic dynein purity at each step.In this study, we make a detailed comparison of the effects of EHNA on 22 different ATP-metabolizing enzyme activities, including 13 Mg++-ATPases. We isolate cytoplasmic dynein-like ATPase activity from three species of sea urchin eggs and sperm and show by means of dose-response curves that their sensitivities to inhibition by EHNA are very similar to one another. We demonstrate further that the EHNA dose-response characteristics of fourteen other ATP-metabolizing enzyme activities, including seven nondynein Mg++-ATPases, differ quantitatively from those of dynein-like ATPases.In studies of three other agents (vanadate, Ca++/calmodulin, and Triton X-100), we find that dynein-like ATPases vary by two orders of magnitude in their sensitivities to inhibition by vanadate, and little or no stimulation by either Ca++/calmodulin or Triton X-100 is seen. Our results suggest that inhibition by EHNA is a universal and specific property of dynein-like ATPases, which ultimately should prove useful in the quantitative purification and characterization of cytoplasmic dynein-like ATPase (s).
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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