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  • Articles  (2)
  • Aboverwaltung  (1)
  • Hybrid identification  (1)
  • Molecular evolution  (1)
  • 1
    German Medical Science GMS Publishing House; Düsseldorf
    In:  GMS Medizin - Bibliothek - Information; VOL: 8; DOC31 /20081218/
    Publication Date: 2008-12-19
    Description: The provision of professional information in hospitals as well as the need of both physicians and purchasers to streamline the process by means of appropriate solutions challenge all players in the value chain. This article describes a case study from the evaluation of the status quo to the implementation of the resulting project steps.
    Description: Die umfassende Literaturversorgung in Kliniken und der gemeinsame Wunsch von Ärzten und Einkauf nach Optimierung durch den Einsatz geeigneter Lösungen stellt alle Betroffenen vor neue Herausforderungen. Der Artikel beschreibt eine Fallstudie von der Evaluierung der Ist-Situation bis zur Umsetzung der einzelnen Projektschritte.
    Keywords: subscription management ; journals ; online access ; provision ; optimization ; Gespag ; Swets ; SwetsWise ; migration to online ; Aboverwaltung ; Zeitschriften ; Online-Zugänge ; Bereitstellung ; Optimierung ; Gespag ; Swets ; Swetswise ; Umstellung auf Online ; ddc: 610
    Language: German
    Type: article
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  • 2
    ISSN: 1432-2242
    Keywords: Hybrid identification ; Molecular evolution ; Repetitive DNA elements ; Satellite DNA ; Solanaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Species-specific repetitive DNA probes are a useful tool for the molecular identification of somatic hybrids. Therefore, the distribution of three repetitive DNA elements of Solanum was investigated in Solanum wild species, Solanum breeding lines, and in more distantly related species of the genera Lycopersicon, Nicotiana, and Datura. The clone pSCH15, obtained from S. circaeifolium, represents a new 168-bp repetitive element; it shows 73–79% sequence similarity to repetitive elements of S. brevidens and Lycopersicon species. The 163-bp element in pSBH6, cloned from S. bulbocastanum, turned out to be very similar (95% sequence homology) to the Lycopersicon element pLEG15/TGRI previously regarded to be present only in species of the genus Lycopersicon and in S. lycopersicoides. Lower sequence similarity of approximately 80% was observed to repetitive elements of S. brevidens which are organized differently. The repeats exhibited different degrees of specificity: by Southern hybridization the element represented by the clone pSBH6 could be detected in almost all Solanum species investigated here but only after long exposure to X-ray film. The previously described “Solanum-specific” element represented by the clone pSA287 was also found, although in a very low copy number, in Lycopersicon esculentum. Therefore, detection of the repetitive elements pSA287 and pSBH6 in those species in which the respective repeat is less represented depends on exposure time. In contrast, the element pSCH15 is prominently present only in a small number of Solanum wild species and — to some extent — in the diploid breeding lines as revealed after long exposure. Use of these repeated elements for the identification of specific genomes in protoplast-fusion hybrids between Solanum wild species and Solanum breeding lines, or between two breeding lines, was evaluated.
    Type of Medium: Electronic Resource
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