Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Alternative splicing  (2)
  • Springer  (2)
  • 1
    ISSN: 1432-1211
    Keywords: Key words Membrane cofactor protein ; Testis ; Chromosome 1 ; Complement ; Alternative splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Human membrane cofactor protein (MCP, CD46) is widely distributed and is one of the plasma membrane complement inhibitors. We isolated cDNA clones encoding genetic homologues of human MCP from a rat testis cDNA library. Northern blot analysis indicated that rat MCP is preferentially expressed in testis, similar to what is found with guinea pig MCP. We identified several different cDNAs, which were presumably generated by alternative splicing from a single-copy gene. The most prevalent isoform corresponded to the Ser/Thr/Pro-rich C type of human MCP. Mouse MCP cDNA was cloned by polymerase chain reaction based on the nucleotide sequence of rat MCP. The deduced amino acid sequence showed 77.8% identity to rat MCP. Mouse MCP was also preferentially expressed in testis. Unique expression in testis in rat and mouse as well as guinea pig suggests that MCPs in these species not only act as complement regulatory proteins but may also have more specialized functions in fertilization or reproduction. Genetic mapping by linkage analysis indicated that the mouse Mcp gene is located on distal chromosome 1, closely linked to the complement receptor 2 (Cr2) gene.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-1211
    Keywords: Key words Decay-accelerating factor ; Transmembrane ; Glycosylphosphatidylinositol ; Complement ; Alternative splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Several cDNA clones encoding genetic homologues of human decay-accelerating factor (DAF) were isolated from rat testis cDNA libraries and reverse transcription-polymerase chain reaction products. Sequence analysis revealed that rat DAF exists as two membrane forms, a glycosylphosphatidylinositol (GPI)-anchored and a transmembrane (TM) form. Southern blotting analysis showed that GPI- and TM-form mRNAs of rat DAF are derived from a single gene, as is the case for guinea pig, though both forms of mouse DAF mRNA are derived from two genes. Gene analysis of the C-terminal region of rat DAF indicated that both forms of mRNA are presumably generated by alternative splicing of exons which encode a GPI/3′-untranslated (3′UT) region and a TM/3′UT region in the 3′ end of the Ser/Thr-rich region. Northern blot analysis indicated that rat GPI-DAF mRNA was present in all tissues examined except for liver, while rat TM-DAF mRNA was preferentially expressed in testis. Infant rat testis barely expressed TM-DAF and membrane cofactor protein (MCP) but highly expressed 5I2 antigen (5I2Ag, rat Crry). However, adult rat testis showed increased expression of the 1.6-kb transcript of rat GPI-DAF, TM-DAF, MCP, and the 0.7-kb transcript of CD59, with a decreased level of 5I2Ag expression. These results suggest that rat 5I2Ag/Crry may play an important role in regulating complement activation during the early stages of testis development, and that DAF, MCP, and CD59 expressed in testis may enable sperm to survive complement attack in the mucus of the female genital organ, and/or to interact with an ovum.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...