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  • 1
    Publication Date: 2015-09-26
    Description: alpha-Chiral amines are key intermediates for the synthesis of a plethora of chemical compounds at industrial scale. We present a biocatalytic hydrogen-borrowing amination of primary and secondary alcohols that allows for the efficient and environmentally benign production of enantiopure amines. The method relies on a combination of two enzymes: an alcohol dehydrogenase (from Aromatoleum sp., Lactobacillus sp., or Bacillus sp.) operating in tandem with an amine dehydrogenase (engineered from Bacillus sp.) to aminate a structurally diverse range of aromatic and aliphatic alcohols, yielding up to 96% conversion and 99% enantiomeric excess. Primary alcohols were aminated with high conversion (up to 99%). This redox self-sufficient cascade possesses high atom efficiency, sourcing nitrogen from ammonium and generating water as the sole by-product.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mutti, Francesco G -- Knaus, Tanja -- Scrutton, Nigel S -- Breuer, Michael -- Turner, Nicholas J -- BB/K0017802/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- New York, N.Y. -- Science. 2015 Sep 25;349(6255):1525-9. doi: 10.1126/science.aac9283.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, Manchester M1 7DN, UK. Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, Manchester M1 7DN, UK. nicholas.turner@manchester.ac.uk f.mutti@uva.nl. ; Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, Manchester M1 7DN, UK. ; BASF SE, White Biotechnology Research, GBW/B-A030, 67056 Ludwigshafen, Germany. ; School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, Manchester M1 7DN, UK. nicholas.turner@manchester.ac.uk f.mutti@uva.nl.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26404833" target="_blank"〉PubMed〈/a〉
    Keywords: Alcohol Dehydrogenase/*chemistry/genetics ; Alcohols/*chemistry ; Amination ; Amines/*chemical synthesis ; Bacillus/enzymology/genetics ; *Biocatalysis ; Genetic Engineering ; Hydrogen/chemistry ; Lactobacillus/enzymology/genetics ; Oxidoreductases Acting on CH-NH Group Donors/*chemistry/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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