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  • Germany  (3)
  • Animal model  (2)
  • 1
    Keywords: RECEPTOR ; EXPRESSION ; Germany ; transcription ; RESPONSES ; INFECTION ; INDUCTION ; ANTIGEN ; CONTRAST ; fibroblasts ; virus ; MOUSE ; BETA ; TRANSFORMATION ; INTERFERON ; ADULT ; RE ; immortalization ; secretion ; MICE LACKING ; INFLAMMATORY RESPONSES ; INTERFERON REGULATORY FACTOR-3 ; ALPHA PRODUCTION ; ANTIVIRAL DEFENSE ; I IFN GENES ; interferon regulatory factor ; mouse fibroblast ; POSITIVE FEEDBACK ; type I interferon ; virus induction
    Abstract: Type I interferons (IFNs) have been shown to be involved in many immune defence and inflammatory responses. We here show that IFN-beta plays an absolute essential role in the efficient induction of all type I IFNs after infection of primary embryonic as well as primary adult fibroblasts with Sendai virus. In contrast, after immortalization of such fibroblasts with SV40 large T antigen, IFN-alpha 4 can be induced independently of IFN-beta. However, efficient secretion of type I IFNs even in immortalized fibroblasts is only found when the complete signalling loop is induced by IFN-beta. (c) 2005 Elsevier Inc. All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 16091286
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  • 2
    Keywords: CANCER ; EXPRESSION ; CELL ; Germany ; IN-VIVO ; THERAPY ; VIVO ; SYSTEM ; TOOL ; liver ; GENE ; GENE-EXPRESSION ; GENES ; PROTEIN ; MICE ; MACROPHAGES ; INDUCTION ; tumour ; BIOLOGY ; culture ; MOUSE ; gene expression ; VECTORS ; PROMOTER ; REGION ; VACCINES ; REGIONS ; DELIVERY ; Jun ; CARRIERS ; GREEN FLUORESCENT PROTEIN ; LUCIFERASE ; CYTOSINE DEAMINASE ; RE ; THERAPIES ; REPORTER GENE ; CARRIER ; HISTOLOGY ; in vivo ; E ; TOOLS ; spleen ; microbiology ; ENGLAND ; host ; FLUORESCENT PROTEIN ; FLUORESCENT ; PLASMIDS ; bacterial ; ARABAD PROMOTER ; tumour therapy
    Abstract: We have used Salmonella enterica serovar Typhimurium (S. typhimurium) which are able to colonize tumours besides spleen and liver. Bacteria were equipped with constructs encoding green fluorescent protein or luciferase as reporters under control of the promoter P-BAD that is inducible with L-arabinose. Reporter genes could be induced in culture but also when the bacteria resided within the mouse macrophages J774A.1. More important, strong expression of reporters by the bacteria could be detected in mice after administration of L-arabinose. This was especially pronounced in bacteria colonizing tumours. Histology demonstrated that the bacteria had accumulated in and close to necrotic areas of tumours. Bacterial gene induction was observed in both regions. P-BAD is tightly controlled also in vivo because gene E of bacteriophage Phi X174 could be introduced as inducible suicide gene. The possibility to deliberately induce genes in bacterial carriers within the host should render them extremely powerful tools for tumour therapy
    Type of Publication: Journal article published
    PubMed ID: 17298393
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  • 3
    Keywords: CANCER ; EXPRESSION ; tumor ; Germany ; IN-VIVO ; THERAPY ; VIVO ; SYSTEMS ; GENE ; GENE-EXPRESSION ; MOLECULES ; TISSUE ; MICE ; INDUCTION ; gene expression ; ESCHERICHIA-COLI ; VECTOR ; PROMOTER ; NETHERLANDS ; BIOLUMINESCENCE ; ENGINEERED BACTERIA ; Gall bladder ; In vivo imaging ; Inducible promoter ; Organ colonization ; Tumor targeted bacteria
    Abstract: The probiotic bacterium Escherichia coli Nissle 1917 (EcN) constitutes a prospective vector for delivering heterologous therapeutic molecules to treat several human disorders. To add versatility to this carrier system, bacteria should be equipped with expression modules that can be regulated deliberately in a temporal and quantitative manner. This approach is called in vivo remote control (IVRC) of bacterial vectors. Here, we have evaluated promoters P-araBAD, P-rhaBAD and P-tet, which can be induced with L-arabinose, L-rhamnose or anhydrotetracycline, respectively. EcN harboring promoter constructs with luciferase as reporter gene were administered either orally to healthy mice or intravenously to tumor bearing animals. Subsequent to bacterial colonization of tissues, inducer substances were administered via the oral or systemic route. By use of in vivo bioluminescence imaging, the time course of reporter gene expression was analyzed. Each promoter displayed a specific ill vivo induction profile depending on the niche of bacterial residence and the route of inducer administration. Importantly, we also observed colonization of gall bladders of mice when EcN was administered systemically at high doses. Bacteria in this anatomical compartment remained accessible to remote control of bacterial gene expression.
    Type of Publication: Journal article published
    PubMed ID: 19665575
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  • 4
    ISSN: 1435-2451
    Keywords: Key words Peritonitis ; Naphthol-AS-d-chloracetat-esterase ; Inflammatory activity ; Animal model ; Schlüsselwörter Peritonitis ; Naphthol-AS-d-Chlorazetatesterase ; Entzündliche Aktivität ; Tiermodell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die entzündliche Aktivität des Peritoneums konnte bisher morphologisch nur semiquantitativ beurteilt werden. In einem standardisierten Rattenmodell wurde eine chronische abszedierende Peritonitis nach Laparotomie und Inokulation von 2 ml einer Bacteroides-fragilis-Suspension mit einer Konzentration von 109CFU/ml induziert. Die entzündliche Aktivität des Peritoneums wurde quantitativ durch die Naphthol-AS-d-Chlorazetatesterase-Färbung (NASDCE) bestimmt, durch die das Zytoplasma der Granulozyten und Gewebsmastzellen markiert wird. Peritonitis- (n=53) und Kontrollgruppe (n=15) wurden randomisiert in 3 Untergruppen eingeteilt (n Peritonitis=17/18/18 vs. n Kontrolle=5/5/5), die für jeweils 3/7/14 Tage beobachtet wurden. An den Tagen 3/7/14 wurden bei 2 von 17, 13 von 18 und 12 von 18 Tieren intraabdominale Abszesse in der Peritonitisgruppe diagnostiziert. Bei den Kontrolltieren wurden keine Abszesse gefunden (p〈0,05). Die Gesamtzellularität und NASDCE-Positivität an den Tagen 3/7/14 betrugen in der Peritonitisgruppe 301/409/280 (vs. 155/240/273 in der Kontrollgruppe) und 1,8/2,9/3,6% (vs. 0,7/0,9/1,4%) im abszeßfernen und 392/661/625 und 14,4/12,9/11,5% im abszeßnahen Kompartiment der Peritonitistiere (p〈0,05). Wir schlußfolgern, daß der qualitative Aspekt einer Perito-nitis z.B. in Form eines Abszesses durch eine quantitative morphologische Charakterisierung der entzündlichen Aktivität des Peritoneums ergänzt werden kann. Durch die NASDCE-Färbung kann der Anteil der Granulozyten und Gewebsmastzellen an der Gesamtzellularität als Hauptindikator der lokalen entzündlichen Aktivität einfach bestimmt werden. Diese Methode kann z.B. hilfreich sein zur Festlegung des Zeitpunkts für den endgültigen Bauchdeckenverschluß bei einer Etappenlavage.
    Notes: Abstract The morphology of the inflammatory activity of the peritoneum has been measured qualitatively but quantitative assessments are not common. In a standardized rat model we induced chronic abscess-forming peritonitis after laparotomy and inoculation of 2 ml Bacteroides fragilis suspension at a concentration of 109/ml colony-forming units. The morphological inflammatory activity was determined quantitatively by staining the specimen of the peritoneum with naphthol-AS-d-chloracetate-esterase (NASDCE); through this staining the cytoplasm of granulocytes and tissue mast cells were marked. The peritonitis group (n=53) and controls (n=15) were randomly divided into three subgroups (n Peritonitis=17/18/18 vs. n control=5/5/5) and observed for 3/7/14 days, respectively. On days 3/7/14 we diagnosed intra-abdominal abscesses in 2 of 17, 13 of 18, and 12 of 18 animals in the peritonitis group. In controls there were no abscesses (P〈0.05). The total cellularity and NASDCE-positive rates on days 3/7/14 in the peritonitis group were 301/409/280 (vs. 155/240/273 in controls) and 1.8/2.9/3.6% (vs. 0.7/0.9/1.4%) in the non-abscess-forming regions and 392/661/625 and 14.4/12.9/11.5% in the abscess-surrounding regions in the infected animals, respectively (P〈0.05). We conclude that the qualitative histological evidence of the morphological inflammatory activity of the peritoneum in the form of an abscess can be supplemented by a quantitative method. Through NASDCE staining the granulocyte and tissue mast cell proportion of the total cellularity as main indicators of the local inflammatory activity can be estimated in peritonitis. This method can be helpful in deciding when to definitively close the abdomen in the course of a programmed lavage treatment in peritonitis.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1435-2451
    Keywords: Peritonitis ; Naphthol-AS-d-chloracetatesterase ; Inflammatory activity ; Animal model ; Peritonitis ; Naphthol-AS-d-Chlorazetatesterase ; Entzündliche Aktivität ; Tiermodell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die entzündliche Aktivität des Peritoneums konnte bisher morphologisch nur semiquantitativ beurteilt werden. In einem standardisierten Rattenmodell wurde eine chronische abszedierende Peritonitis nach Laparotomie und Inokulation von 2 ml einerBacteroides-fragilis-Suspension mit einer Konzentration von 109CFU/ml induziert. Die entzündliche Aktivität des Peritoneums wurde quantitativ durch die Naphthol-AS-d-Chlorazetatesterase-Färbung (NASDCE) bestimmt, durch die das Zytoplasma der Granulozyten und Gewebsmastzellen markiert wird. Peritonitis- (n=53) und Kontrollgruppe (n=15) wurden randomisiert in 3 Untergruppen eingeteilt (n Peritonitis=17/18/18 vs.n Kontrolle=5/5/5), die für jeweils 3/7/14 Tage beobachtet wurden. An den Tagen 3/7/14 wurden bei 2 von 17, 13 von 18 und 12 von 18 Tieren intra-abdominale Abszesse in der Peritonitisgruppe diagnostiziert. Bei den Kontrolltieren wurden keine Abszesse gefunden (p〈0,05). Die Gesamtzellularität und NASDCE-Positivität an den Tagen 3/7/14 betrugen in der Peritonitisgruppe 301/409/280 (vs. 155/240/273 in der Kontrollgruppe) und 1,8/2,9/3,6% (vs. 0,7/0,9/1,4%) im abszeßfernen und 392/661/625 und 14,4/12,9/11,5% im abszeßnahen Kompartiment der Peritonitistiere (p〈0,05). Wir schlußfolgern, daß der qualitative Aspekt einer Peritonitis z.B. in Form eines Abszesses durch eine quantitative morphologische Charakterisierung der entzündlichen Aktivität des Peritoneums ergänzt werden kann. Durch die NASDCE-Färbung kann der Anteil der Granulozyten und Gewebsmastzellen an der Gesamtzellularität als Hauptindikator der lokalen entzündlichen Aktivität einfach bestimmt werden. Diese Methode kann z.B. hilfreich sein zur Festlegung des Zeitpunkts für den endgültigen Bauchdeckenverschluß bei einer Etappenlavage.
    Notes: Abstract The morphology of the inflammatory activity of the peritoneum has been measured qualitatively but quantitative assessments are not common. In a standardized rat model we induced chronic abscess-forming peritonitis after laparotomy and inoculation of 2 mlBacteroides fragilis suspension at a concentration of 109/ml colony-forming units. The morphological inflammatory activity was determined quantitatively by staining the specimen of the peritoneum with naphthol-AS-d-chloracetate-esterase (NASDCE); through this staining the cytoplasm of granulocytes and tissue mast cells were marked. The peritonitis group (n=53) and controls (n=15) were randomly divided into three subgroups (n Peritonitis=17/18/18 vs.n control=5/5/5) and observed for 3/7/14 days, respectively. On days 3/7/14 we diagnosed intra-abdominal abscesses in 2 of 17, 13 of 18, and 12 of 18 animals in the peritonitis group. In controls there were no abscesses (P〈0.05). The total cellularity and NASDCE-positive rates on days 3/7/14 in the peritonitis group were 301/409/280 (vs. 155/240/273 in controls) and 1.8/2.9/3.6% (vs. 0.7/0.9/1.4%) in the non-abscess-forming regions and 392/661/625 and 14.4/12.9/11.5% in the abscess-surrounding regions in the infected animals, respectively (P〈0.05). We conclude that the qualitative histological evidence of the morphological inflammatory activity of the peritoneum in the form of an abscess can be supplemented by a quantitative method. Through NASDCE staining the granulocyte and tissue mast cell proportion of the total cellularity as main indicators of the local inflammatory activity can be estimated in peritonitis. This method can be helpful in deciding when to definitively close the abdomen in the course of a programmed lavage treatment in peritonitis.
    Type of Medium: Electronic Resource
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