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  • 1
    Keywords: OPTIMIZATION ; RECEPTOR ; CELLS ; IN-VITRO ; CELL ; COMBINATION ; VITRO ; GENE ; GENES ; BIOLOGY ; ASSAY ; CELL-LINE ; LINE ; NETHERLANDS ; RECEPTORS ; ER ; signaling ; SCIENCE ; methods ; ANDROGEN RECEPTOR ; CHEMICALS ; ANTAGONISTS ; ANDROGEN ; androgens ; in vitro ; reporter gene assay ; RANGE ; ANDROGEN-RECEPTOR ; PANEL ; Prevalidation ; ENDOCRINE DISRUPTORS ; REPORTER ; Hershberger assay ; Androgen reporter gene assay ; BIOASSAYS ; HUMAN CELL-LINE
    Abstract: To date there are no validated methods available to test androgenicity or antiandrogenicity in vitro. A problem with testing androgenicity using reporter genes is the possibility by other steroid receptors than androgen receptors to activate the same reporter gene, thereby lowering selectivity. To avoid this we have established a robust and very selective method, the AR CALUX reporter gene assay, to test androgenic and antiandrogenic activity of compounds in vitro. This assay uses a human U2-OS cell line stably transfected with the human androgen receptor and an androgen receptor responsive reporter gene. We optimized protocols to be used in combination with AR CALUX cells and carried out an in house prevalidation. In addition we successfully transferred this assay to another laboratory, leading to comparable test results with a panel of androgen receptor agonists and antagonists. The assay was able to readily rank a range of chemicals on the basis of their EC50 values. The CALUX assay was found to be selective for androgens and seemed not influenced by signaling through other steroid receptors.
    Type of Publication: Journal article published
    PubMed ID: 20438827
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  • 2
    ISSN: 1433-0407
    Keywords: Schlüsselwörter Farbkodierte Duplex-Sonographie ; Fibromuskuläre Dysplasie ; intimale Variante ; A. carotis ; Key words Ultrasonography ; -doppler ; -color ; Fibromuscular-dysplasia-diagnosis ; Carotid-artery-diseases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Traditionally, intra-vitam diagnosis of fibromuscular dysplasia (FMD) of brain supplying arteries is a domain of conventional selective angiography. We are aware of only two publications reporting of ultrasound displayed intimal and medial variants of carotid artery FMD, in one case each. We present a patient in whom color-coded duplex-sonography revealed a web-like carotid artery bulb stenosis meeting the specific criteria of a subtype of intimal FMD.
    Notes: Zusammenfassung Die intra vitam Diagnose der fibromuskulären Dysplasie (FMD) hirnversorgender Arterien gilt als eine Domänie der selektiven Katheterangiographie. Bislang liegen nur zwei Mitteilungen über die Diagnose der FMD durch B-Bild- beziehungsweise Duplex-Sonographie der A. carotis vor, eine davon mit Darstellung eines membranösen intimalen Subtyps der FMD. Wir konnten bei einem Patienten die spezifischen Kriterien einer septalen intimalen FMD des Bulbus caroticus Duplex-sonographisch dokumentieren.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1433-8491
    Keywords: Depression ; Antidepressant drug ; Drop attack ; Movement disorder ; Myoclonus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Six patients experienced falling spells concurrent with the intake of antidepressant drugs in high doses or in moderate doses combined with other psychotropic drugs. The close temporal correlation between drug ingestion and the falling spells and their immediate termination after reduction or discontinuation of medication suggests that these drugs caused the falls. Further factors predisposing to the falling spells remain to be defined.
    Type of Medium: Electronic Resource
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