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  • 1
    Publication Date: 2011-12-14
    Description: NF-kappaB is crucial for innate immune defence against microbial infection. Inhibition of NF-kappaB signalling has been observed with various bacterial infections. The NF-kappaB pathway critically requires multiple ubiquitin-chain signals of different natures. The question of whether ubiquitin-chain signalling and its specificity in NF-kappaB activation are regulated during infection, and how this regulation takes place, has not been explored. Here we show that human TAB2 and TAB3, ubiquitin-chain sensory proteins involved in NF-kappaB signalling, are directly inactivated by enteropathogenic Escherichia coli NleE, a conserved bacterial type-III-secreted effector responsible for blocking host NF-kappaB signalling. NleE harboured an unprecedented S-adenosyl-l-methionine-dependent methyltransferase activity that specifically modified a zinc-coordinating cysteine in the Npl4 zinc finger (NZF) domains in TAB2 and TAB3. Cysteine-methylated TAB2-NZF and TAB3-NZF (truncated proteins only comprising the NZF domain) lost the zinc ion as well as the ubiquitin-chain binding activity. Ectopically expressed or type-III-secretion-system-delivered NleE methylated TAB2 and TAB3 in host cells and diminished their ubiquitin-chain binding activity. Replacement of the NZF domain of TAB3 with the NleE methylation-insensitive Npl4 NZF domain resulted in NleE-resistant NF-kappaB activation. Given the prevalence of zinc-finger motifs and activation of cysteine thiol by zinc binding, methylation of zinc-finger cysteine might regulate other eukaryotic pathways in addition to NF-kappaB signalling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, Li -- Ding, Xiaojun -- Cui, Jixin -- Xu, Hao -- Chen, Jing -- Gong, Yi-Nan -- Hu, Liyan -- Zhou, Yan -- Ge, Jianning -- Lu, Qiuhe -- Liu, Liping -- Chen, She -- Shao, Feng -- England -- Nature. 2011 Dec 11;481(7380):204-8. doi: 10.1038/nature10690.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="" target="_blank"〉PubMed〈/a〉
    Keywords: Adaptor Proteins, Signal Transducing/chemistry/*metabolism ; Bacterial Secretion Systems ; Cysteine/*metabolism ; Enteropathogenic Escherichia coli/metabolism/pathogenicity ; Escherichia coli Proteins/*metabolism ; Humans ; Intracellular Signaling Peptides and Proteins/chemistry/*metabolism ; MAP Kinase Kinase Kinases/metabolism ; Methionine/analogs & derivatives/metabolism ; Methylation ; Methyltransferases/metabolism ; NF-kappa B/*antagonists & inhibitors/*metabolism ; Protein Binding ; Protein Structure, Tertiary ; Signal Transduction ; Substrate Specificity ; TNF Receptor-Associated Factor 6 ; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism ; Ubiquitin/*metabolism ; Virulence Factors/*metabolism ; Zinc Fingers
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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