Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • thermal diffusivity  (3)
  • Bi-based superconductor  (2)
  • DOWN-REGULATION  (2)
  • 1
    Keywords: RECEPTOR ; KINASE ; PROTEIN ; DOWN-REGULATION ; fibroblasts ; BONE-MARROW ; STROMAL CELLS ; INTERNATIONAL-SOCIETY ; THERAPY POSITION STATEMENT ; GENOME-WIDE RNAI
    Abstract: Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting.
    Type of Publication: Journal article published
    PubMed ID: 26120366
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: EXPRESSION ; MICE ; DOWN-REGULATION ; METHYLATION ; METHYLTRANSFERASE ; NEPHROGENIC DIABETES-INSIPIDUS ; AQUAPORIN-2 TRAFFICKING ; WATER CHANNELS ; ENAC-ALPHA ; SET DOMAIN
    Abstract: Dot1l encodes histone H3 K79 methyltransferase Dot1a. Mice with Dot1l deficiency in renal Aqp2-expressing cells (Dot1l(AC)) develop polyuria by unknown mechanisms. Here, we report that Aqp5 links Dot1l deletion to polyuria through Aqp2. cDNA array analysis revealed and real-time RT-qPCR validated Aqp5 as the most upregulated gene in Dot1l(AC) vs. control mice. Aqp5 protein is barely detectable in controls, but robustly expressed in the Dot1l(AC) kidneys, where it colocalizes with Aqp2. The upregulation of Aqp5 is coupled with reduced association of Dot1a and H3 dimethyl K79 with specific subregions in Aqp5 5' flanking region in Dot1l(AC) vs. control mice. In vitro studies in IMCD3, MLE-15 and 293Tcells using multiple approaches including real-time RT-qPCR, luciferase reporter assay, cell surface biotinylation assay, colocalization, and co-immunoprecipitation uncovered that Dot1a represses Aqp5. Human AQP5 interacts with AQP2 and impairs its cell surface localization. The AQP5/AQP2 complex partially resides in the ER/Golgi. Consistently, AQP5 is expressed in none of 15 normal controls, but in all of 17 kidney biopsies from patients with diabetic nephropathy. In the patients with diabetic nephropathy, AQP5 colocalizes with AQP2 in the perinuclear region and AQP5 expression is associated with impaired cellular H3 dimethyl K79. Taken together, these data for the first time identify Aqp5 as a Dot1a potential transcriptional target, and an Aqp2 binding partner and regulator, and suggest that the upregulated Aqp5 may contribute to polyuria, possibly by impairing Aqp2 membrane localization, in Dot1l(AC) mice and in patients with diabetic nephropathy.
    Type of Publication: Journal article published
    PubMed ID: 23326416
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1572-9605
    Keywords: Bi-based superconductor ; doping ; XPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract The Bi2Sr2CaCu2O8+δ system samples doped with Sn on Cu sites were synthesized. Resistivity temperature dependence, X-ray powder diffraction and photoemission experiments have been performed. Suppression of superconductivity in the Sn-doped Bi2Sr2CaCu2O8+δ system has been observed. X-ray photoemission measurements show that Sn ion enters the lattice. Our results reveal that Sn ions are mostly in bivalent states.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1572-9567
    Keywords: composites ; copper ; flash method ; iron ; thermal diffusivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A method for measuring thermal diffusivity in one of the layers of a two-layer composite sample has been described. The heat transfer problem of a two-layer sample associated with pulse thermal diffusivity measurements has been analyzed for two cases: exponential and square-wave pulses. According to our measurements, a triangular heat-pulse function approximates reasonably well the output of the Nd-glass laser. In this paper, an expression is derived for the temperature transient at the rear face of two-layer sample being subjected to a triangular heat-pulse input on the front face. The analytical solution of the problem forms the basis of our method of data reduction. This solution has been programmed for computer processing of the data. The method described here has been successfully tested by limited measurements on copper and iron.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1572-9567
    Keywords: anisotropic materials ; pyrolytic graphite ; pyrolytic boron nitride ; thermal conductivity ; thermal diffusivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The laser pulse method can be successfully applied to the measurement of thermal diffusivity of isotropic materials subject to some assumptions. For anisotropic materials, this method is applicable to the measurement of principal thermal diffusivity only on the condition that there is no difference in direction between the principal axis and that of the temperature gradient. After analyzing the heat conduction process in an anisotropic solid, it has been shown that large errors in the measurement of thermal diffusivity would exist if the direction of the principal axis deviates inconspicuously from that of the temperature gradient. The experimental results of thermal diffusivity of highly oriented pyrolytic graphite (HOPG) samples with various deviation angles have been compared with the analytical results. The laser pulse method is not applicable to measurements on semitransparent pyrolytic boron nitride (PBN). We adopted a two-layer composite sample to measure the thermal diffusivity of PBN in the c direction and a particular graphite-PBN composite sample has been prepared which has a very low thermal resistance at the interface. The thermal diffusivity and thermal conductivity of PG (below 2300°C) and PBN (below 1000°C) are given.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1572-9567
    Keywords: molten carbonates ; molten nitrate ; thermal conductivity ; thermal diffusivity ; transient short-hot-wire technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A transient short-hot-wire technique has been successfully used to measure the thermal conductivity and thermal diffusivity of molten salts (NaNO3, Li2CO3/K2CO3, and Li2CO3/Na2CO3) which are highly corrosive. This method was developed from the hot-wire technique and is based on two-dimensional numerical solutions of unsteady heat conduction from a short wire with the same length-to-diameter ratio and boundary conditions as those used in the actual experiments. In the present study, the wires are coated with a pure Al2O3 thin film by using a sputtering apparatus. The length and radius of the hot wire and the resistance ratio of the lead terminals and the entire probe are calibrated using water and toluene with known thermophysical properties. Using such a calibrated probe, the thermal conductivity and thermal diffusivity of molten nitrate are measured within errors of 3 and 20%, respectively. Also, the thermal conductivity of the molten carbonates can be measured within an error of 5%, although the thermal diffusivity can be measured within an error of 50%.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1572-9605
    Keywords: Bi-based superconductor ; doping ; PES ; XRD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract The Bi2Sr2CaCu2O8+δ system samples doped with Pr on Ca sites were synthesized. Resistivity measurements, x-ray powder diffraction, and photoemission experiments were performed. The experiment results show that Pr ions entered the lattice and chemical environment plays a major role in the shift of core-level spectra and valence-band spectra.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...