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  • 1
    Online Resource
    Online Resource
    New York, NY : Springer New York | New York, NY : Springer New York
    Subject(s): Life sciences ; Biochemistry ; Life sciences ; Biochemistry, general
    In: Springer eBooks
    Type of Medium: Online Resource
    Pages: XII, 373 p. 79 illus., 64 illus. in color. , online resource.
    Edition: 2nd ed. 2016.
    ISBN: 9781493937714
    Series Statement: Methods in Molecular Biology, 1451
    Language: English
    Note: Chemical Screening in Zebrafish -- TALEN-Mediated Mutagenesis and Genome Editing -- Homology Independent Integration of Plasmid DNA into the Zebrafish Genome -- Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System -- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish -- Testing of cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish using PhiC31 Integrase -- Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP Positive Cells from Transgenic Zebrafish Lines -- Construction of the Inbred Strain -- Zebrafish as a Model for the Study of Solid Malignancies -- Melanoma Regression and Recurrence in Zebrafish -- Imaging of Human Cancer Cell Proliferation, Invasion and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model -- Modeling Leukemogenesis in the Zebrafish using Genetic and Xenograft Models -- Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos -- Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae -- Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish -- Studying Lipid Metabolism and Transport During Zebrafish Development -- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish -- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy -- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain -- Quantifying Aggressive Behaviour in Zebrafish -- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish -- A Practical Guide to Light Sheet Microscopy -- Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish -- Fiber Optic-Based Photostimulation of Larval Zebrafish -- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.
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