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  • Articles  (2)
  • Arachidonic acid  (1)
  • Blood-brain barrier  (1)
  • 1995-1999  (2)
  • 1960-1964
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  • Articles  (2)
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  • 1995-1999  (2)
  • 1960-1964
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Topic
  • 1
    ISSN: 1572-9699
    Keywords: Arachidonic acid ; isolation ; Mortierella ; soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Malt extract agar and an incubation temperature of 5 °C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6903
    Keywords: Blood-brain barrier ; hypothalamic extract ; astrocyte ; capillary endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this study was to investigate the influence of hypothalamic extract, astrocyte co-culture, and astrocyte-conditioned medium on the barrier function of an in vitro model of the blood-brain barrier. Porcine brain capillary endothelial cells were grown on polycarbonate membranes suspended between two chambers of media, representing the capillary lumen and brain interstitium. Endothelial cells grown alone and cocultured with astrocytes were cultured in growth medium with or without 50 μg/mL hypothalamic extract. An additional treatment consisted of endothelial cells cultured in growth medium that was first conditioned by astrocytes. Coculture consisted of a noncontact model with astrocytes attached to the bottom of the abluminal chamber. Barrier function of the endothelial cells was tested on days 1 through 9 post-seeding by measuring permeability to macromolecules (albumin) and small ions (electrical resistance). Resistance to the passage of macromolecules and small ions was greatest for endothelial cells grown without astro-cytes in growth medium supplemented with hypothalamic extract. This barrier was maximal during days 4 through 7 post-seeding and was significantly less permeable than the barrier formed by endothelial cells grown in un-supplemented growth medium, in coculture with astrocytes, or in astrocyte-conditioned medium. These results demonstrate that a noncontact coculture with astro-cytes did not enhance the integrity of this in vitro BBB model employing porcine brain capillary endothelial cells, but barrier function was increased when the model's medium was supplemented with hypothalamic extract.
    Type of Medium: Electronic Resource
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