Springer Online Journal Archives 1860-2000
Abstract This study examines the putativegastroprotective effect of adenosine onindomethacininduced gastric lesions and the possiblemechanisms involved. After 24 hr of starvation, the ratswere treated either with indomethacin (Indo; 25 mg/kg,subcutaneously) alone or adenosine + Indo (Ado; 7.5mg/kg, subcutaneously, three times a day), or thevehicle (5% NaHCO3, subcutaneously). Thelength of hemorrhagic lesions in the stomachs was expressed as the lesionindex. The tissue-associated myeloperoxidase (MPO)activity and protein oxidation were measured in gastrictissue samples. Formation of reactive oxygen species in gastric tissues was measured by usingluminol- and lucigenin-enhanced chemiluminescence. Inother groups of rats, gastric mucosal permeability andgastric acid output were performed following the same treatment regimens. The gastric mucosalpermeability was measured by determination of[51Cr]EDTA clearance in a perfused stomachpreparation and gastric acid secretion studies wereperformed following pylorus ligation. The lesion index, the increase inlucigenin-enhanced chemiluminescence, and the increasein gastric mucosal permeability in Indo-treated ratswere reversed by Ado pretreatment. Ado pretreatment also prevented the increase in gastric acidoutput and gastric volume in Indo-treated rats. Thus,these findings implicate that exogenous adenosine has aprotective role on indomethacin-induced gastric lesions, possibly by inhibiting gastrichyperacidity and reactive oxygen formation and bypreventing disruption of the mucosalintegrity.
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