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  • Analytical Chemistry and Spectroscopy  (2)
  • Cardiac muscle cells  (1)
  • United States
  • 1985-1989  (3)
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Year
  • 1
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The ratio of the fragment ions at m/z 122 and m/z 123 in the positive ion fast atom bombardment or secondary ion mass spectra of thiamine hydrochloride varies with sample preparation and experimental conditions. For all mass spectra that contained significant abundances of matrix (S) ions [S + H]+, the fragment at m/z 123 is the more abundant of the two ions. If [S + H]+ ions are not observed in the mass spectrum under the conditions selected, the ion at m/z 122 is more abundant. This correlation suggests that hydrogen transfer to the fragment ion occurs in the gas phase, with the composition of the ion-solvent cluster ions in the selvedge region being the key factor. The ratio of the fragment ions at m/z 123 and m/z 122 is thus an indicator of the extent of protonation in the selvedge, the region immediately above the solvent surface created by primary particle bombardment.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Cardiac muscle cells ; Extracellular matrix ; Collagen fibers and filaments ; Immunohistochemistry ; Rat ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphology, composition, and function of struts that interconnect the lateral surfaces of cardiomyocytes were examined in the hearts of rats and hamsters. Methods included brightfield and fluorescent light microscopy, secondary and backscatter scanning electron microscopy, and transmission electron microscopy in conjunction with silver stain, cationic dye, and antibody to type-I collagen. These studies reveal a twisted, beaded appearance and a complex substructure of collagen fibrils embedded in a ground substance that has a positive reaction with cationic dye. A hierarchy of patterns of branching and attachment was seen among intercellular struts ranging in diameter from 0.1 μm to several urn. The hypothesis that struts tether not only the surfaces but the contractile lattices of laterally adjacent myocytes is supported by the following: (a) the attachments of struts to the collagen weave of the sarcolemma, often lateral to the level of Z bands, (b) the presence of collagen type I in a composite material arrangement, (c) the relative dispositions and configurational changes of struts and myocyte surfaces in various physiological states and induced, non-physiological perturbations of cardiac muscle, (d) the corrugated sarcolemmas with infoldings near Z bands, and (e) the continuity of intracellular filaments from Z bands to the inner aspect of the sarcolemma in relaxed and contracted myocytes. Implications of struts acting as tethers and sites for storage of energy in the motions of myocytes during the cardiac cycle are discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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