di-ortho substituted polychlorinated biphenyls (PCBs)
2-(1-Pyrenyl)ethyldimethylsilylated silica column
Analytical Chemistry and Spectroscopy
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
A Method has been developed for the separation and enrichemen of there non-ortho, eight mono-ortho, and di-ortho substituted polychlorinated biphenyls (PCBs) from Aroclor formulations and environmental samples. The fractionation is accomplished using high performance liquid chromatography (HPLC) with a 2-(1-pyrenyl)ethyldimethylsilysily silca column. GC-MSD with an optimized temperature program was used for quantitation, Hexane, pentane, cyclohexane, iso-octane, and 2-propanol were tested as a mobile phase for the isolation of the thirteen target PCBs in a Aroclor 1242, 1254, and 1260 (1:1:1) misture, Pentane at room temperature with a slow rate of 0.7 ml/min is the condition of choice. The average recovery of thirteen target PCBs spiked in the Aroclor mixture is 99.5% with an average relative standard deviation of 4.5%. The average method detection limit is 8pg/μl. Targer PCBs in the reference solis, incinarator ash, and sediment samples were measured.
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