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  • Chemistry  (1)
  • Zinc  (1)
  • 1
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A catalase has been purified from aerobically grown Escherichia coli K12. The enzyme exhibits unorthodox properties compared with catalyse from bovine liver and seems to be identical to hydroperoxidase II from E. coli. A mathematical model is presented which makes it possible to calculate the steady-state concentration of hydrogen peroxide in an open system. The model has been verified experimentally. It has been shown that the catalase from E. coli is better suited than the bovine liver enzyme for oxygen supply to cell suspensions using hydrogen peroxide.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-4909
    Keywords: Key words Heavy metal bioprecipitation ; Heavy metal biosensors ; Heavy metal resistance ; Cadmium ; Cobalt ; Zinc ; Nickel ; Chromate ; Ralstonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In contrast to thermophilic or psychrophilic organisms, heavy metal-resistant bacteria do not supply enzymes that are active under harsh conditions, but are themselves tools for the evaluation and remediation of heavy metal-contaminated environments. Ralstonia sp. CH34 is a gram-negative bacterium with a remarkable set of resistance determinants, allowing this bacterium to live in extreme environments that are heavily contaminated with toxic metal ions. These heavy metal ions are mostly detoxified by inducible ion efflux systems that reduce the intracellular concentration of a given ion by active export. Because all metal resistance determinants in this bacterium are inducible, their regulatory systems can be used to develop biosensors that measure the biologically important concentrations of heavy metals in an environment. Resistance based on metal ion efflux detoxifies only the cytoplasm of the respective cell. Therefore, this resistance mechanism cannot be used directly to develop biotechnological procedures; however, metal ion efflux can protect a cell in a metal-contaminated environment. Thus, the cell can be enabled to mediate biochemical reactions such as precipitation of heavy metals with the carbon dioxide produced during growth or degradation of xenobiotics.
    Type of Medium: Electronic Resource
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