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  • Chemistry  (71)
  • 1
    Keywords: Chemistry ; Biotechnology ; Nanotechnology ; Chemistry ; Biotechnology ; Nanotechnology ; Springer eBooks
    Description / Table of Contents: Nanoparticle-GFP “Chemical Nose” Sensor for Cancer Cells Identification -- A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism using a DNA Nanosensor -- A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells using a Fluorescent Dye -- Gold Nanoparticle as a Marker for Precise Localization of Nano-objects within Intracellular Sub-Domains -- Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation -- Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments -- Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains -- A Method to Encapsulate Molecular Cargo within DNA Icosahedra -- Delivery of Plasmid DNA to Mammalian Cells using Polymer-Gold Nanorod Assemblies -- Lipophilic Formulated Gold Porphyrin Nanoparticles for Chemotherapy -- Mitochondria-specific Nano-Emulsified Therapy for Myocardial Protection against Doxorubicin-induced Cardiotoxicity -- Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events -- Characterization of Nanoparticle – Lipid Membrane Interactions using QCM-D -- Single-Cell Nanosurgery -- Single Quantum Dot Imaging in Living Cells -- Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging -- Monitoring the Degradation of Reduction Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry -- Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces -- Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery -- Real-Time Particle Tracking for Studying Intracellular Trafficking of Pharmaceutical Nanocarriers -- Interactions of Nanoparticles with Proteins -Determination of Equilibrium Constants -- Tracing the Endocytic Pathways and Trafficking Kinetics of Cell Signaling Receptors using Single QD Nanoparticles -- Cellular Internalization of Quantum Dots -- Electrochemical Scanning Tunneling Microscopy and Spectroscopy for Single Molecule Investigation -- Intracellular Delivery of Biologically Active Proteins with Peptide-based Carriers -- Lipo-oligoarginine-based Intracellular Delivery -- Fluorescent Spherical Monodisperse Silica Core-shell Nanoparticles with a Protein-binding Biofunctional Shell -- Direct Quantification of PTD Transduction Using Real-Time Monitoring -- Genotoxic Assessment of Carbon Nanotubes -- Separation Science: Principles and Applications for the Analysis of Bionanoparticles by Asymmetrical Flow Field-Flow Fractionation (AF4) -- Polymersomes-mediated Delivery of Fluorescent Probes for Targeted and Long Term Imaging in Live Cell Microscopy -- Protocol for the Preparation of Stimuli-responsive Gold Nanoparticles Capped with Elastin-based Pentapeptides
    Abstract: In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biologý„Ø series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ℗ Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences
    Pages: XVI, 370 p. 90 illus., 52 illus. in color. : digital.
    ISBN: 9781627033367
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 16 (1997), S. 69-71 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The management of safety, quality and environmental issues covers a complex set of interrelated issues of concern to society and industry. Traditionally an engineering approach has been adopted for the management of risk; the development of technical standards, operations and requirements. Recently, industry leaders have begun to realize that real progress will be made only throgh effective risk management systems. This paper presents a few of the key requirements for improving risk management and risk analysis. By improving the way risk analysis is used for decision support, improving hazard identification exercise, while incorporating site specific properties of design, condition, operation and management into an analysis, it is possible to improve the value of current risk management techniques.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 512-518 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pseudomonas putida biofilms were developed on and biofilm accumulation rate data were obtained for the following two classes of support materials: charged surfaces and noncharged hydrophobic and hydrophilic surfaces. The effects of surface roughness and porosity on the rate of microbial attachment were also examined.Materials bearing a net positive or negative surface charge supported the greatest biofilm accumulation and the highest biofilm accumulation rate. Uncharged hydrophobic materials achieved the next greatest biofilm accumulation, averaging approximately 50% of the total biomass which was accumulated on the charged surface materials after 16 days. Uncharged hydrophilic materials supported very little biofilm development. In general, biofilm accumulation increased with decreased surface roughness. The effect of pore size on biofilm accumulation was not conclusive.The biofilm accumulation kinetics showed an exponential accumulation rate for the charged surfaces and an approximately linear accumulation rate for the hydrophobic materials. This difference in accumulation kinetics is consistent with proposed differences in the physicochemical mechanism governing attachment to these two types of surface materials.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1040-0397
    Keywords: Glucose ; Composite electrodes ; Pulsed amperometry ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Segregated composite electrodes mimic microelectrode ensembles. In this preliminary report, the use of a rotating gold-polychlorotri- fluoroethylene (or Kel-F, a 3M Company polymer) composite electrode in combination with pulsed amperometric detection (PAD) is described for the detection of glucose. Comparisons are made with results obtained at a solid gold disk electrode. The composite electrode exhibits a higher signal and a lower background than does the solid gold electrode. In terms of current density, the enhancement of the signal above the background is over 3-fold, similar to that observed with segregated graphite composite electrodes used in a constant potential mode. Little or no glucose signal is observed at either the solid gold or the gold composite electrode when employed in the constant potential mode. In the PAD mode, the signal is stable for periods in excess of an hour.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Process Safety Progress 13 (1994), S. 59-60 
    ISSN: 1066-8527
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1040-0397
    Keywords: Uranium ; Sensor ; Remote sensing ; Field screening ; Modified electrode ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A carbon paste electrode modified with the ligand propyl gallate is introduced for preconcentration/voltammetric measurements of low levels of uranium. The resulting preconcentrating electrode offers many attractive properties for on-site sensing of uranium, including high sensitivity and selectivity, self-cleaning capability, and use of nondeaerated quiescent solutions. The response is characterized with respect to the paste composition, preconcentration period, solution and differential pulse conditions, and other variables. The sensor displays high stability, and can be used repeatedly without regeneration. When coupled to a 100-ft (30.5-m) long shielded cable, the electrode offers a remote sensing capability, as illustrated in the determination of uranium in groundwater.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Am System Formaldehyd/Isobutyraldehyd wird gezeigt, daß bei der säurekatalysierten Aldolreaktion, die bezüglich des Aufbaueffektes der basenkatalysierten Reaktion entspricht, aus den dimeren Aldolen tricyclische Acetale mit dem Ringsystem des 2.6.10.14.17.18-Hexaoxa-tricyclo-[11.3.1.15.9]-octadecans entstehen.  -  Bei der Selbstkondensation des Formaldehyds in Eisessig/Schwefelsäure bildet sich Acetylglykolsäure.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Perhydroxymethylierte Formaldole kondensieren, infolge des die Reaktion beherrschenden Anionotropie-Effektes, erst nach einer partiellen Entaldolisierung mit anderen reaktionsfähigen Carbonylverbindungen, Partiell hydroxymethylierte Formaldole kondensieren nach dem gleichen Verknüpfungsprinzip wie Mannich-Basen. - Aus dem 3.5.5-Tris-hydroxymethyl-dihydro-desoxy-patulinsäurelacton entstand bei der Behandlung mit Schwefelsäure das Methylen-bis-〈3.6-dioxo-2.9-dioxa-spiro[4.5]decan〉 und daraus durch Aldolaufbau mit Paraformaldehyd/Salzsäure das 3.6.10.13-Tetraoxo-2.14.17.20.23-pentaoxa-tetraspiro[4.1.1.1.4.3.3.3]tetracosan. - Bei der Umsetzung des 3.5.5-Tris-hydroxymethyl-dihydro-desoxy-patulinsäurelactons mit überschüssigem Acetessigester und einer geringen Menge Natrium erhielt man Spiro[5-oxo-tetra-hydrofuran-3.1′ -6′-carbäthoxy-7′.9′-dihydroxy-Δ6′-3′-oxa-decalin], das bei der Ketonspaltung in das Spiro[5-oxo-tetrahydrofuran-3.1′-7′-oxo-Δ8′-3′-oxa-decalin] überging.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The master two-dimensional gel database of human keratinocytes currently lists 2980 cellular proteins (2098 isoelectric focusing, IEF; and 882 nonequilibrium pH gradient electrophoresis, NEPHGE) many of which correspond to posttranslational modifications. About 20% of all recorded proteins have been identified (protein name, organelle components, etc.) and they are listed in alphabetical order together with their Mr, pI, cellular localization and credit to the investigator(s) that aided in the identification. Also, we have listed 145 microsequenced proteins that are recorded in this database. As an aid in localizing the polypeptides we have included blow-ups of the master images (IEF, NEPHGE) displaying all the protein numbers. In the long run, the master keratinocyte database is expected to link protein and DNA sequencing and mapping information (Human Genome Program) and to provide an integrated picture of the expression levels and properties of the thousands of proteins that orchestrate various keratinocyte functions both in health and disease.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0173-0835
    Keywords: Keratinocytes ; Differentation ; Abnormal regulation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Comparison of the protein expression patterns of proliferating normal primary human keratinocytes plated in serum-free medium (SFKM), supplemented with epidermal growth factor (EGF) and bovine pituitary extract (BPE), and similar cultures induced to differentiate by the addition of Dulbecco's modified Eagle medium (DMEM), containing 10% fetal calf serum (FCS), revealed several known and unknown polypeptides that are abnormally regulated in the differentiated cells. Upregulated proteins included keratins (keratins 6, 10/11, 14 and 16), members of the S100 protein family psoriasin, MRP8, MRP14 and S100c), actin-binding proteins (gelsolin and tropomyosin 9220), annexins (annexins IV and VIII), hsp28, the fatty acid binding protein 5 (FABP5), the squamous cell carcinoma (SCC) antigen, members of the 14-3-3 family, involucrin, E-cadherin, cystatin A, desmoglein and integrins α2 and β1, as well as several proteins of as yet unknown identity. The highest upregulated proteins corresponded to psoriasin (124.0 times), MRP8 (42.4 times), MRP14 (14.9 times), tropomyosin 9220 (11.5 times), involucrin (11.1 times), and FABP5 (9.1 times). FABP5, hsp28, and tropomyosin 9220 were also highly upregulated in quiescent keratinocytes indicating that their increased levels in the differentiated cell may be due to loss of proliferative activity. Highly downregulated proteins included PAI-2, tropomyosins 9213, 9121 and 9122, keratin 5, calnexin, 14-3-3 beta and eta, nucleoside diphosphate kinase A, Rho GDIs, hsp60, hnRNPs H and C2, α-enolase, eIF-4D, thioredoxin, annexins III and V, moesin, nucleolar protein B23, GST π and PCNA/cyclin. Both the high expression of keratin 6 and 16 - which are markers for an alternative pathway of keratinocyte differentiation - as well as the extremely high upregulation of some members of the S100 protein family indicate that the cells have differentiated via an abnormal pathway.
    Additional Material: 4 Ill.
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