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  • 1
    ISSN: 1432-072X
    Keywords: Conidiobolus coronatus ; UV-20 variant strain ; Conidial discharge ; Serine proteases ; Phenyl methyl sulfonyl fluoride inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The involvement of serine protease(s) in the conidial discharge of Conidiobolus coronatus was investigated using the parent strain and a variant strain with reduced conidial discharge. Time course profiles of protease levels and conidial discharge showed that maximum protease levels coincided with maximum conidial discharge in both the parent and variant strains. Inhibition of serine protease(s) by phenyl methyl sulfonyl fluoride showed that low protease levels resulted in inhibition of the conidial discharge and a minimum activity of 1.0 U/mg protein is essential for triggering the conidial discharge. Using casein to induce proteases, it was further observed that early gain in the protease level (1.0 U/mg protein) leads to early onset of conidial discharge. The above evidence suggests the involvement of protease(s) in the conidial discharge of C. coronatus.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0972
    Keywords: Conidiobolus coronatus ; extracellular alkaline phosphatase ; phenylalanine ; phenylglycine ; resolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An isolate ofConidiobolus coronatus (NCIM 1238) showed high proteinase activity (20.1 U/ml) and productivity (600 U/l.h) when 1% (w/v) glucose or sucrose was used as the carbon source in shake flasks. Addition of organic nitrogen sources, casein (2%), soybean flour (4%), liver extract (2%) or Edamin-S (2%), enhanced growth and proteinase production up to three-fold and seven-fold, respectively. The system was successfully run up to 6 l in a laboratory fermenter with a productivity of 600 U/l.h. The proteinase was successfully used to resolve the recemic mixtures ofdl-phenylalanine anddl-phenylglycine and thus could replace the currently used subtilisin.
    Type of Medium: Electronic Resource
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