Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • DNA flowcytometry  (3)
  • CANCER CELLS  (2)
  • 1
    Keywords: HUMAN-BREAST ; MEDIA ; MMP-9 ; tumor microenvironment ; E ; THERAPIES ; SERUM ; PROGRESSION ; prevention ; BREAST-CANCER ; breast cancer ; culture ; BREAST ; microenvironment ; CANCER-CELLS ; SERA ; THERAPY ; human ; tumor ; CELL ; CANCER ; CANCER CELLS ; CELLS
    Type of Publication: Book chapter
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    Keywords: RECEPTOR ; CANCER ; CANCER CELLS ; CELLS ; EXPRESSION ; IN-VITRO ; INVASION ; proliferation ; SURVIVAL ; tumor ; CELL ; human ; KINASE ; PATHWAY ; PATHWAYS ; SUPPORT ; EXPOSURE ; SITE ; DIFFERENTIATION ; MONOCLONAL-ANTIBODY ; NF-KAPPA-B ; ACTIVATION ; FAMILY ; BINDING ; PHOSPHORYLATION ; ALPHA ; BREAST ; breast cancer ; BREAST-CANCER ; antibodies ; MEMBRANE ; METASTASIS ; REGION ; CANCER-CELLS ; EXTRACELLULAR-MATRIX ; BETA ; ADHESION ; MIGRATION ; FACTOR-KAPPA-B ; INTEGRIN ; NF-kappa B ; RECEPTORS ; MMP ; FIBRONECTIN ; TUMOR-METASTASIS ; SERUM ; FAMILIES ; basement membrane ; cell adhesion ; GELATINASE-A ; MMP-2 ; INTEGRINS ; RGD ; NUCLEAR ; EVENTS ; ERK ; MMP-9 ; pharmacology ; MT1-MMP ; TISSUE INHIBITOR ; ENGLAND ; matrix metalloproteinase ; MATRIX-METALLOPROTEINASE ; MEDICINE ; MEDIA ; interactions ; MT1-MMP MMP-14 ; PI-3K
    Abstract: Interactions between tumour cells and the extracellular matrix (ECM) strongly influence tumour development, affecting cell survival, proliferation and migration. Many of these interactions are mediated through a family of cell surface receptors named integrins. Fibronectin and its integrin receptors play important roles in tumour development. The alpha 5 beta 1 integrin interacts with the central cell adhesive region of fibronectin and requires both the RGD and synergy sites for maximal binding. Matrix metalloproteinases (MMPs) are a family of zinc dependent endopeptidases. They are capable of digesting the different components of the ECM and basement membrane. The ECM gives structural support to cells and plays a central role in cell adhesion, differentiation, proliferation and migration. Binding of ECM to integrins modulates expression and activity of the different MMPs. Our experimental findings demonstrate that cultivation of human breast cancer cells, MCF-7, in serum free medium in the presence of fibronectin upregulates the activity of MMP-2 and MMP-9. Blocking of alpha 5 beta 1 integrin with anti-alpha 5 monoclonal antibody inhibits the fibronectin-induced MMP activation response appreciably. This strongly indicates alpha 5 beta 1 mediated signalling events in activation of MMP-2 and MMP-9. Phosphorylation of FAK and PI-3 kinase and the nuclear translocation of ERK and NF-kappa B upon fibronectin binding demonstrate possible participation of the FAK/PI-3K/ERK signalling pathways in the regulation of MMP-2 activity. (c) 2007 Elsevier Inc. All rights reserved
    Type of Publication: Journal article published
    PubMed ID: 18243246
    Signatur Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1437-9813
    Keywords: Key words Unilateral undescended testis ; Rat ; Testicular injury ; DNA flowcytometry ; Anti-sperm antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was designed to evaluate whether creation of a unilateral undescended testis (U/L UDT) in rats by direct fixation of the testis can lead to changes in the contralateral (C/L) descended testis, and if so, whether this inherent problem of the model could be eliminated by anchoring the divided gubernaculum to indirectly fix the testis. Thirty male newborn rats were divided into three groups of 10 each and the procedure done on the 2nd day of life to create U/L UDT according to the group allocated: group I: sham-operated; group II: anchoring the gubernaculum after gubernaculectomy; group III: Direct suture fixation of the testis. Fertility, C/L testicular weight (TW), Johnsen score, seminiferous tubular diameter (STD), DNA flowcytometry, and serum anti-sperm antibodies (ASA) were studied. Fertility, C/L TW, Johnsen score, STD, and haploid cell population were significantly reduced in group III compared to group II, while significantly higher titers of ASA were found in group III. Gubernaculectomy and anchoring the gubernaculum to the anterior abdominal wall is a better technique of creation of experimental UDT, as direct fixation of the testis is potentially detrimental to the C/L normal, descended testis.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1437-9813
    Keywords: Key words Fertility ; Unilateral undescended testis ; DNA flowcytometry ; Anti-sperm antibodies ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Continuing experimental work on the effect of experimentally created unilateral undescended testis (UL-UDT) in neonatal rats, this study examined the fertility and correlated it to contralateral (CL) testicular morphology, seminiferous tubular diameter (STD), DNA flowcytometry, and the presence of serum anti-sperm antibodies (ASA) at 120–135 days of age. In our previous reported work, the fertility of rats with UL-UDT at 65–80 days of age was the same as that of controls. In the present study the rats with UL-UDT had significantly reduced fertility (P 〈 0.01) compared to controls, even though the Johnsen scores and mean STD of the CL testicular tissue were comparable. DNA flowcytometry demonstrated a significant decrease (P 〈 0.001) in haploid cell population in the CL testicular tissue of rats with UL-UDT. Furthermore, the rats with UL-UDT who either received an immunosuppressive or in whom the UDT was excised early showed almost normal fertility and DNA histograms like those of controls. Significantly high titres of serum ASA were detected only in the group with UL-UDT when tested at 135 days of age. From these results, in combination with earlier results on similar work, it may be inferred that UL-UDT causes immunologically-mediated, progressive damage to the CL descended testis, leading to a decrease in fertility in rats.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1437-9813
    Keywords: Key words Cyclosporine ; Male fertility ; DNA flowcytometry ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of cyclosporine (CsA) on fertility has assumed greater importance with the increasing numbers of pediatric transplantations being performed all over the world. Conflicting reports on the effects of CsA on sex hormones are available. This experimental animal study was designed to examine the effect of CsA on testicular weight, sperm counts, seminiferous tubular diameter (STD), testicular morphology, DNA flowcytometry, sex hormone levels, and fertility in male rats. Those rats who received CsA (20 mg/kg per day) showed significant reductions in testicular weight (P 〈 0.05), sperm count (P 〈 0.01), Johnsen score (P 〈 0.05), STD (P 〈 0.01), serum testosterone levels (P 〈 0.05), haploid cell population (P 〈 0.001) in the testis, and fertility (P 〈 0.001) compared to those receiving CsA 10 mg/kg per day and control rats. These findings will have an important bearing for children receiving cyclosporine for long periods to guide the physician in optimally adjusting long-term treatment.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...