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  • 1
    ISSN: 1432-2242
    Keywords: Quercus petraea ; Q. robur ; Q. pubescens ; Diversity ; Bootstrap ; Autocorrelation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast DNA polymorphisms have been detected by the conventional Southern-blotting hybridization method in four species of European oaks (Quercus petraea, Q. robur, Q. pubescens and Q. pyrenaica). Three polymorphisms, shared by at least three of these species, can be scored directly in ethidium bromidestained gels and were used in a broad survey of the level of differentiation of the oak species and of their pattern of genetic structure in western Europe. The highly significant geographic variation and the high genetic differentiation (Gst=0.895, SGst=0.025) indicate a low level of cytoplasmic gene flow. We conclude that cytoplasmic genomes are well suited for the reconstruction of past migrational routes of such a complex of species.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 462-470 
    ISSN: 1432-2242
    Keywords: Diversity ; Differentiation ; Variance ; Optimal design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An extension of Nei's analysis of diversity in a subdivided population is proposed for a haploid locus. The differentiation G STbecomes a natural extension of Wright's F STand generalizes Weir and Cockerham's parameter of co-ancestry by relaxing the assumption of identical correlation for all the alleles. Inter- and intrapopulation variances of the estimated diversities and differentiation are derived. Finally, the optimal sampling strategy for measuring G STwhen a fixed number of individuals can be analysed is considered. It is shown that, at a given locus, there is a unique sample size per population which yields the smallest variance of G ST,regardless of the number of populations studied. These theoretical developments are illustrated with an analysis of chloroplast DNA diversity in a forest tree. The results emphasize the necessity of sampling many populations, rather than many individuals per population, for an accurate measurement of the subdivision of gene diversity at a single locus.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1432
    Keywords: Maritime pine ; 2D electrophoresis ; Genetic variability ; Diversity ; Organ-specific polypeptides ; Mobility variants ; Molecular weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using two-dimensional polyacrylamide gel electrophoresis, the genetic variation of proteins was examined in three organs (needle, bud, and pollen) from 18 trees of maritime pine. Three types of variation were noted: presence/absence, staining intensity, and position variation of the spots. Of the 902 polypeptides scored in the three organs, 245 (27.2%) were polymorphic. Moreover, among these variable spots, 117 were found in a single organ, demonstrating an increased polymorphism of the organ-specific polypeptides (56.0% vs 18.4% for the organ-unspecific polypeptides). Finally, a positive correlation was found between variability level and subunit molecular weight for spots showing position variation but not for spots showing presence/absence or staining intensity variations. Possible explanations for this observation are discussed.
    Type of Medium: Electronic Resource
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