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  • COMPLEX  (1)
  • Early embryogenesis  (1)
  • 1
    Keywords: CANCER ; Germany ; human ; CLASSIFICATION ; QUANTIFICATION ; DISEASE ; DISEASES ; PROTEIN ; PROTEINS ; COMPLEX ; RESPONSES ; COMPLEXES ; INFECTION ; RISK-FACTORS ; ANTIGEN ; FLOW ; papillomavirus ; ASSOCIATION ; antibodies ; antibody ; virus ; IN-SITU ; ASSAY ; GLUTATHIONE ; NUMBER ; REPRODUCIBILITY ; CERVICAL-CANCER ; FUSION ; FUSION PROTEINS ; human papillomavirus ; HPV ; E6 ; case-control studies ; LINKED-IMMUNOSORBENT-ASSAY ; glutathione-S-transferase ; FUSION PROTEIN ; E6 ONCOPROTEIN ; SERUM ; CHEMISTRY ; ELISA ; case-control study ; PROGRAM ; RE ; case control studies ; multiplex ; MICROSPHERE IMMUNOASSAY
    Abstract: Background: More than 100 different human papillo-maviruses (HPVs) can cause proliferative diseases, many of which are malignant, such as cervical cancer. HPV serology is complex because infection and disease lead to distinct type-specific antibody responses. Using bead-based technology, we have developed an assay platform that allows the simultaneous detection of antibodies against up to 100 in situ affinity-purified recombinant HPV proteins. Methods: Twenty-seven HPV proteins were expressed as glutathione S-transferase fusion proteins and affinity-purified in one step by incubation of glutathione-displaying beads in bacterial lysate. Spectrally distinct bead sets, each carrying one particular antigen, were mixed, incubated with serum, and differentiated in a flow cytometer-like analyzer (xMAP; Luminex Corp). Antibodies bound to the antigens were detected via fluorescent secondary reagents. We studied 756 sera from 2 case-control studies of cervical cancer. Results: Glutathione S-transferase fusion proteins bound with high affinity to glutathione-displaying beads (K-d = 6.9 X 10(-9) mol/L). The dynamic range of multiplex serology covered 1.5 orders of magnitude, and antibodies were detected at serum dilutions 〉 1:1 000 000. Imprecision (median CV) was 〈= 5.4%, and assay reproducibility was high (R-2 = 0.97). Results on clinical samples showed high concordance with ELISA (kappa = 0.846), but multiplex serology exhibited increased detection of weak antibody responses. Antibodies to the E6 oncoproteins of the rare HPV types 52 and 58 were associated with cervical cancer (P 〈 0.001). Conclusion: Multiplex serology enables antibody analyses of large numbers of sera against up to 100 antigens in parallel and has the potential to replace ELISA technology. (c) 2005 American Association for Clinical Chemistry
    Type of Publication: Journal article published
    PubMed ID: 16099939
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  • 2
    ISSN: 0925-4773
    Keywords: Early embryogenesis ; Extracellular matrix ; Integrin ; Xenopus
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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