Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Keywords: CANCER ; EXPRESSION ; IN-VITRO ; tumor ; carcinoma ; COMBINATION ; Germany ; PROSTATE ; VITRO ; POPULATION ; CDNA ; GENE ; GENE-EXPRESSION ; GENES ; GENOME ; microarray ; PROTEIN ; RNA ; transcription ; TISSUE ; MECHANISM ; BIOMARKERS ; mechanisms ; DOWN-REGULATION ; PROGRESSION ; gene expression ; microarrays ; prostate cancer ; PROSTATE-CANCER ; LOCALIZATION ; CARCINOMAS ; CDNA MICROARRAYS ; microdissection ; prostate carcinoma ; QUANTITIES ; MANAGEMENT ; CDNA MICROARRAY ; HETEROGENEITY ; molecular ; RADICAL PROSTATECTOMY ; EXTRACTION ; PROTOCOL ; MOLECULAR-MECHANISMS ; biomarker ; RECOVERY ; RNAS ; PRESERVATION ; HIGH-GRADE ; NEEDLE BIOPSIES ; ALTERNATE READING FRAME ; COA RACEMASE P504S ; ISCHEMIA TIME ; laser microdissection ; tissue banking
    Abstract: Molecular analyses of early-stage prostate cancers are necessary to assess their potential clinical significance based on established and/or novel biomarkers for tailored clinical management. A prerequisite for the application of RNA-based analyses of such, mostly macroscopically-undetectable, small prostate carcinomas is the recovery and preservation of sufficient RNA quantities and quality. Furthermore, in prostate cancer, heterogeneity is a common phenomenon that includes a juxtaposition of different tissue compositions and variable histological grades within the same tumor focus. To better understand the molecular mechanisms of prostate cancer, it is essential to correlate molecular data with a specific cell type. Here, we present a tissue collecting protocol which is aligned with the preoperative evaluation of tumor localization. In combination with the technique of laser microdissection and pressure catapulting, we are able to preserve RNA of high quality from homogeneous cell populations of macroscopically-undetectable small prostate carcinomas. To obtain the necessary RNA quantities for whole genome cDNA microarrays, the isolated total RNAs were amplified by T7-based RNA-polymerase in vitro transcription. The microarray analyses (Human Unigene Set RZPD3.1) resulted in 216 differentially expressed genes (191 down-regulated, 25 Up-regulated). Among these were several known prostate cancer relevant genes, such as AMACR, TARP, LIM, GPR160 (all up-regulated), CAVI, NTNI, MTIX; CLU, TRIM29, SPARCLI and HSPB8 (,all down-regulated)
    Type of Publication: Journal article published
    PubMed ID: 16077921
    Signatur Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-2277
    Keywords: Key words Liver graft quality ; Effluates ; Prediction of survival ; Glutathione S-transferase ; Glutamate dehydrogenase ; Leucocyte count
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Because transplantation success is influenced by the quality of the graft, the objective of this study was to find parameters to evaluate transplant livers in the recipient centre. In 64 liver grafts, the venous effluates of a portal back-table flush were investigated for various parameters. Amongst them, glutathione S-transferase (GST), glutamate dehydrogenase (GLDH) and the leucocyte count were found superior in predicting graft survival. Using the combination of these parameters, 100-day graft survival of between 95 % (all parameters positive) and 0 % (all parameters negative) was predicted. We concluded that good liver grafts are characterized by a low width of injury (cytosolic component: GST), a low depth of injury (mitochondrial component: GLDH), as well as by a potential to induce tolerance (passenger leucocytes). Perfusate analysis seems to be a valuable tool to recognize problematic grafts in advance and to quantify the “graft factor” in considerations concerning quality control.
    Type of Medium: Electronic Resource
    Signatur Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...